LOCUS       sequence-288976-        8078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot4_187-1051.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8078)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot4_187-1051_I
FEATURES             Location/Qualifiers
     source          1..8078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5363..5404
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5414..5431
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5454..5471)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5504..5638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5528..5547)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5582..5601
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6260..6848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6340..6359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7019..7810,7811..7879))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7642..7661
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7880..7984)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8052..8070
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgct cgaggatgac
     2761 cagcttgggg acaaccaaat actgtagcca cttcatgaag aaccagcagt gtcccaagag
     2821 cgactgcatg tacttacatg aattgggcga tcccgaggcc agtttcacaa aggaggaaat
     2881 gcatcaggga aagcacctgg agtacgagaa gcgcctgcac gatactctaa ttgcgtcatt
     2941 gggaccgaat gcaacaggta ttgtaaatgt cgcgattcca tcgtcatcgt cagcgtcatc
     3001 ctcgtcctct ggatcgggta caaatggttc aagtgcatca ggtaatgccc agcaaaagga
     3061 ggcctggcca agcttatcgg tctcgcccat caatggcaaa gaggcggctg ccactgcgac
     3121 cagttccagt gggaaaagta aacgggagaa gctgcgcaac gagaagaggc acgagaagaa
     3181 caaggcgaag aataagaacg gcagcaatac aaacgccaat gcctcaaaca aggagaacta
     3241 tgttcccgaa acgagaagca gcacaagtac tgagacattc gcagaggcta cggcggatgc
     3301 accggcttcc accaaggcag aaccgccgca agcctctagc aatagaacga gggcggatcg
     3361 tggaaaagat cggaccacgg ctagtgcaaa ggagcagaag aagggcaagg aaactgctcc
     3421 agcacctgca gcaagtaaac cggcggagcg ggttgaaaca agcgagagta caataagaca
     3481 aaagaaggcg gaagtaaccg aaagctgtga agataactta ccacaaaaga gattagcggg
     3541 aacaaacgtt caaagatctg tgagctcttg tagcgaaaat agcgaaggac acgtctctga
     3601 gagtagctta agtgagaaga gtttaactgg tgattatgtg gaggaaaagt gcaatagtgt
     3661 gaattcggaa agccagcaag aaagtgtaaa gtttcaggag gagctcgaaa agagcaacga
     3721 ggcaattgtc gaggccgaaa cgattctgcc aacagctgag tcctctgagg acatcagtcc
     3781 cgctgcagtt gcgccaggca atggagaaga cgaaggatgc ttacccgttg ttgatccagt
     3841 cgaaccaccg agtctggcgg ataatggaag cagagtaaca gatgcgctat ctaagctgaa
     3901 cattttcgat gacacgccta gctttttcac atcgccatca ttccagcaag cacccatatt
     3961 aaagaataag ctagatttgg aaatgcgaca gtctcattta cctgacttgg tcaacgacat
     4021 tgatggaatc caaaaggcat ccaatacaaa cgagtgggag gaagccttta aaaatgtgat
     4081 gatgggcaac acgcagcaca tggaggagca actgttgcag cagcaacatt tgcagcagca
     4141 tcagaatttg cgccaccagc tggtgttgca acaggaggag ttccttcgca tgcaagaatt
     4201 acagaaacgc aacaactttg cgacgcaaat caacggtcct gcgaatgatt tccttagagc
     4261 ttatgagctg agagctcaag ccaatgcaat cattcagcaa caattattac aacagcacgc
     4321 aggcgaaaat ctatttggcg gcaacatgtc gaagttcttc gatttccata aaagtcagcc
     4381 gcagtcacac catcagtatc taaatggaca tccgcctcaa atcaatggga atggtgctgt
     4441 gccggagccg caaagagtgg cggcctcttt ggaaagcaat cgactgaatt cgccttttgt
     4501 cgaaaatgga cttattaact cgcagcagca gcagcagcag cagcaacagc aacaaaagca
     4561 gagaatgatg ggaatgtatg aatttatgcc tccaaacacg caatctcagc agaatcggtt
     4621 tacacagaac tcgatagtcg acgatgactt aggattcgac ccgttcgttg agacccaaaa
     4681 gggacttgct gaacttatgg aaaatgaggt ggtccaaaaa cagagtatta ataatgagaa
     4741 ccccttgccg aagttgccgc cacagcctca agttccaccc catccgcaat tggtggacaa
     4801 cctgcagcga gctcgcatgc cgccgccagg cttcaatcac gtcaacacat tgggcttggg
     4861 cggtgcatcc agactgcagc tcaccagcaa aataatgccc ttcatgaaca tgcccgttaa
     4921 tggggtgggc aacagcggtg cccagggaca gcaccaaatg ccaatgggtg tcaactggaa
     4981 tgcgccgatg ggcatgcacc agaacccggg acagcccgtg ggtgattcgc aattgcagca
     5041 tccaatggct cacaacaagg tttacaacaa cagtgattgg acttcaatgg atccggctat
     5101 actttcgttt agacagtatt cttcttttcc acaaaaccaa attcccccac atcctcagca
     5161 gcaacaggat ttatttttgc agcatttggc tcaacagcaa aattcccaga gtggtggttt
     5221 caacaaccag gcacagcaaa tgcttcctat ggggatgccc aatagtttgc tgaacggaca
     5281 acaaacgcag ccgccacagg tcaatgccaa tgttcagggc atgcttgagt ttttaaaaag
     5341 ccgtcaattc gtttagttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     5401 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
     5461 tgtgccttct aaggcctgag ctcgctgatc agcctcgatc gaggatccag acatgataag
     5521 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     5581 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5641 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     5701 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagtcga ccgatgccct
     5761 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     5821 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
     5881 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
     5941 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
     6001 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
     6061 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     6121 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     6181 gacagcttca aggatcgctc gcggctctta ccagccagca aaaggccagg aaccgtaaaa
     6241 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6301 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6361 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6421 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     6481 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6541 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6601 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6661 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     6721 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6781 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6841 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6901 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6961 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7021 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7081 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7141 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7201 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7261 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7321 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7381 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7441 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7501 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7561 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7621 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7681 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7741 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7801 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7861 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7921 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7981 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8041 taacctataa aaataggcgt atcacgaggc cctttcgt
//
