LOCUS       sequence-288989-       10744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6Biso1-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 10744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6Biso1-siRNAres_I
FEATURES             Location/Qualifiers
     source          1..10744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6448..6569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6459..6478)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6513..6532
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6755..7210
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6842..6861)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7052..7073
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7327..7684
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7327..7518
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7350..7370)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7535..7670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7735..8529
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7789..7808)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8399..8418
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8593..8641
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8682..8701)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8801..8823
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8861..8879)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8947..9051
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9052..9120,9121..9912)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9270..9289)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10083..10671
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10572..10591
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agaattcacg cgtggtacct ctagagatgg
     1201 cctacccata tgatgttcca gattacgcta tgagagagaa ggagcaagaa agggaagaac
     1261 agttaatgga agacaagaaa aggaagaaag aggataaaaa gaaaaaagaa gccactcaga
     1321 aggtcacgga acaaaaaacc aaagtgcccg aagtgacgaa accaagttta agccaaccaa
     1381 cggccgccag cccaattggc agctctccat cgccaccagt caatggtggc aacaatgcca
     1441 aaagggtggc agtgccgaac ggacaaccgc caagcgccgc ccgctacatg cctcgggagg
     1501 tgccgccgcg attccgttgc cagcaggacc acaaagtgtt actaaaacgt gggcagcccc
     1561 ctccaccgtc ctgcatgctc cttgggggtg gggcagggcc tcctccctgc acagcacctg
     1621 gagcaaaccc aaacaacgca caagtgacag gagcgctgct gcagagtgag agtgggactg
     1681 cgccagactc aacccttgga ggtgctgctg cttcaaatta tgcaaattcc acttggggct
     1741 cgggagcctc ctccaacaac ggcacctccc ccaacccaat tcacatctgg gacaaggtga
     1801 ttgtagacgg gtctgacatg gaagagtggc cctgcatcgc tagtaaagac actgaatctt
     1861 cttccgaaaa caccaccgat aacaacagtg cctcgaaccc tggctctgag aagagcactc
     1921 tgccaggaag caccactagt aacaaaggaa aagggagcca gtgccagtct gcaagttctg
     1981 ggaacgaatg taatcttggg gtctggaaat ctgaccctaa ggctaaatct gttcaatctt
     2041 ccaactctac tacagagaac aacaatggac taggaaattg gaggaatgtg agtggtcagg
     2101 atagaattgg acctggctct ggcttcagca actttaaccc aaatagcaac ccatctgcct
     2161 ggccagcact ggtccaagaa ggaacttcta ggaaaggggc attggaaaca gataatagta
     2221 attccagtgc acaggttagc acagtaggtc agacatccag ggaacagcag tcaaagatgg
     2281 aaaatgcggg tgttaatttt gttgtctctg gcagagaaca ggctcaaatt cataacactg
     2341 atggaccaaa aaatggaaac actaactcct tgaacttaag ttcaccaaac cccatggaga
     2401 ataagggaat gccctttgga atgggcttgg ggaacacctc caggagcact gatgcccctt
     2461 cacaaagcac tggagatcga aagactggga gtgttggatc ttggggtgca gctagggggc
     2521 cttctggaac tgacacagtc tctggacaaa gcaattctgg aaacaatggg aacaatggaa
     2581 aagagagaga ggactcctgg aaaggagctt ctgttcagaa atcaactggg tcaaaaaatg
     2641 actcttggga caacaataac aggtctacgg gtgggtcctg gaactttggc ccccaggact
     2701 ctaatgacaa caaatggggt gaagggaaca aaatgacatc tggggtctct cagggagaat
     2761 ggaaacagcc gactgggtct gatgagttga aaattggaga atggagtggt ccaaaccaac
     2821 caaattctag cactggagca tgggacaatc aaaagggcca ccccctccct gaaaaccaag
     2881 gcaatgccca ggctccctgt tggggaagat cttccagctc cacaggaagt gaagttggag
     2941 gtcaaagcac tggaagcaac cacaaagcag gaagtagtga cagtcataac tctggccgtc
     3001 ggtcgtacag gcccacacat cctgattgtc aggctgtctt gcagactctt ttgagccgaa
     3061 ctgatttgga ccccagggtg ctctcaaaca ctggctgggg ccaaactcaa attaagcagg
     3121 acacagtgtg ggacattgaa gaggtgccaa ggcctgaggg gaaatctgac aaaggaactg
     3181 aggggtggga gagcgctgcc acacagacca agaactcagg gggctgggga gatgcaccca
     3241 gccaaagcaa tcaaatgaag tctggatggg gggagctctc agcctctaca gagtggaaag
     3301 accccaagaa cacaggaggc tggaatgact acaagaacaa caactcttcc aactggggag
     3361 gaggacgacc tgatgaaaag acaccttcct cttggaatga gaatcccagc aaggatcagg
     3421 ggtggggagg tggacgccag cccaatcaag gatggtcttc tggaaagaat ggttgggggg
     3481 aggaagtcga tcagacaaaa aacagcaatt gggaaagttc tgcaagtaaa cctgtgtctg
     3541 ggtggggtga aggagggcag aatgaaatcg ggacttgggg taatggtggc aatgcaagcc
     3601 tagcttcaaa aggtgggtgg gaggattgca aaagatcccc agcatggaat gagacgggcc
     3661 gacagcccaa ttcctggaat aaacaacacc aacagcagca gcccccacag cagccgccgc
     3721 caccacaacc agaggcttct ggttcgtggg gaggcccacc cccaccacct ccaggcaacg
     3781 ttcgaccttc caattccagc tggagcagcg ggccacagcc tgcaacacct aaggatgagg
     3841 aacccagtgg ttgggaagag ccatccccac agtcaattag tcggaaaatg gacattgatg
     3901 atggcacttc agcatgggga gaccctaaca gttataacta caagaatgtg aatctgtggg
     3961 ataagaattc ccaagggggc ccagcacctc gagaaccaaa cctgcccacc ccaatgacca
     4021 gtaaatcggc atcagattcc aaatctatgc aagacggctg gggggagagt gacgggccag
     4081 tcacaggagc tcgccatccc agctgggaag aggaggagga tggaggagtc tggaacacca
     4141 ctggctctca gggcagtgct tcctcccaca actcagcaag ctggggacaa ggaggaaaga
     4201 aacaaatgaa gtgctcactc aaaggaggaa acaatgattc atggatgaat cctcttgcca
     4261 aacagttttc aaatatggga ttgctgagtc agactgaaga taatccaagc agcaaaatgg
     4321 atttgtctgt aggaagcctt tcagataaaa aatttgatgt ggacaagcga gcgatgaatc
     4381 tcggggattt taatgatatc atgaggaagg atcgatctgg gttccgtcca cctaattcca
     4441 aagacatggg aaccacagat agtgggcctt attttgagaa gggcggtagt catggtttgt
     4501 ttggaaacag cacagcacaa tcgagaggtc tgcacacacc cgtgcagcca ctaaattctt
     4561 ctcccagtct ccgggcgcaa gtgcctcccc agtttatttc cccccaggtt tctgcctcaa
     4621 tgctcaagca gtttcccaac agtggcctga gtccaggtct tttcaatgtg gggccccagt
     4681 tatctcctca acaaattgcc atgctgagcc agcttccaca aattccccag tttcagttgg
     4741 catgtcagct tctcttgcag cagcagcaac agcagcagtt gttacagaac cagagaaaga
     4801 tttctcaagc tgtacgccaa cagcaagagc agcagctggc tcgaatggtg agtgcactgc
     4861 agcagcagca gcagcagcag cagaggcagc caggcatgaa gcactcgccc tctcatcctg
     4921 ttgggcccaa gccgcatctg gacaacatgg tacccaacgc attgaatgtg gggctcccag
     4981 accttcaaac caaagggcca atacctggat atggttctgg cttcagctct ggcggcatgg
     5041 actatggcat ggttggtggg aaggaggctg gaaccgagtc tcgctttaaa cagtggacct
     5101 ccatgatgga ggggctgccc tctgtagcca cacaggaagc caatatgcac aaaaatggcg
     5161 ctatagtggc ccctggtaaa acccggggag ggtcaccgta caaccagttt gatatcatcc
     5221 ctggtgacac actgggtggc catacgggtc ctgctggtga tagctggtta cctgccaaat
     5281 ctccaccaac aaataaaatc ggaagtaaat ccagcaatgc cagttggcct ccagaattcc
     5341 aaccaggggt cccttggaag ggaatccaaa acattgaccc tgaatctgac ccctatgtca
     5401 ccccaggaag tgtgctgggg ggtacagcca catctcccat tgtagatact gaccaccaac
     5461 tgctgcggga taacaccaca gggtctaatt cttccctcaa cacctcgctg ccttcacctg
     5521 gtgcctggcc ctacagtgcc tctgacaact cctttaccaa cgttcatagc acttcagcaa
     5581 agttccctga ttacaaatca acatggtccc cagatcccat aggacacaac cccactcatc
     5641 tctccaacaa gatgtggaaa aaccatattt cctccaggaa cactacaccg ctgccccgcc
     5701 cacctcctgg tctgaccaac cccaaaccat catctccctg gagcagcaca gcaccccgat
     5761 cagtcagggg gtgggggaca caggactcac ggctcgcctc ggcctctacc tggagtgatg
     5821 gtggctcagt tcgtcctagt tactggctgg ttcttcacaa tctcacccca cagattgatg
     5881 ggtcaacctt gagaacgatc tgcatgcagc atggcccact gctgacattc catctgaatc
     5941 taacccaggg cactgccctg atccgataca gcaccaaaca ggaggcggcc aaggcccaaa
     6001 ctgcactgca catgtgtgtg ttgggaaaca ctaccatcct tgctgagttt gccactgatg
     6061 atgaagtcag ccgctttctg gcacaagctc agccccctac acctgcagca accccaagtg
     6121 cgccagctgc ggggtggcag tcgctggaga ccggccagaa ccagtcagat cccgtgggac
     6181 ctgctctgaa tctttttggt gggtccactg ggctcgggca gtggagcagc agcgctggtg
     6241 gcagcagcgg ggccgatctt gctggcgctt cattgtgggg gcccccaaac tattcttcta
     6301 gcttatgggg agtcccaacg gtggaagatc cccataggat gggcagccct gctcctttac
     6361 tacctggtga ccttctggga ggagggtcgg attcaatctg agcggccgct tccctttagt
     6421 gagggttaat gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     6481 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6541 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6601 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6661 aaatccgata aggatcgatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc
     6721 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     6781 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6841 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6901 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6961 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7021 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7081 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7141 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7201 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     7261 tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     7321 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7441 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7501 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7561 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7621 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7681 caaaaagctt gattcttctg acacaacagt ctcgaactta aggctagagc caccatgatt
     7741 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7801 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7861 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7921 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7981 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     8041 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     8101 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     8161 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     8221 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     8281 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     8341 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8401 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8461 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8521 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     8581 cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     8641 gaatcgatag cgataaggat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc
     8701 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     8761 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     8821 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     8881 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8941 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9001 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9061 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9121 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9181 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9241 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     9301 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9361 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9421 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9481 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9541 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     9601 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9661 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9721 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9781 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     9841 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9901 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9961 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10021 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10081 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10141 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10201 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10261 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10321 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10381 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10441 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10501 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10561 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10621 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10681 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag
    10741 atct
//
