LOCUS       sequence-289023-       10487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmeIF4Ga.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 10487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I
FEATURES             Location/Qualifiers
     source          1..10487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7772..7813
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7823..7840
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7863..7880)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7913..8047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7937..7956)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7991..8010
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8669..9257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8749..8768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9428..10219,10220..10288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10051..10070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10289..10393)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10461..10479
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgcagcaagc
     2761 aatccctaca ctacctactc agtcggacat cgacaaggct atgcaaccac actcggctca
     2821 gaacatgtac gtgtctacag gtaataataa ctcaggtaac acgcggtcaa acccgcagag
     2881 cggtgggatt ttccgtgggc ctccatctac accaaacgct ccaaggggag caagcggagg
     2941 ggctacgcga cacgtgcacg ttcagccaat gtattcgcag ccattgcatc aaaatatggt
     3001 tctccagcag tatacgcagt acaatcctcg acagcagacc ttcccggcat cacacttaca
     3061 gtatgccccg gctccgatgc cctattatca atatcagtac gtgcccacat tgcaacagca
     3121 acctcctcac acacggagcg ccgtgactgt gaacactaat gtgaacgtag ggaataacct
     3181 ccaacccgtt caatcaggcc caaacggccc attgccagtc ccaggcgcca gttcttctca
     3241 gatacagctt ttgacgagta cggtacaacc tggtgctagt acagtgatgg gagtgggagg
     3301 accaggcagc acgatgggac aggtaggagt cccccccatg gtcggtgtgg gagtgatgac
     3361 tacgagcgtt cagccacagc cggtacaagt ccaacccgcc agtcgtcggc gacaccaaca
     3421 ccgccttcaa atcatagacc ccacaactaa aaaaaatatt ttagacgatt ttgataagac
     3481 aaaatcaaac acagacaatg aattttccga tcaggtaacc tccacaaaca caccagcaac
     3541 tgtcctttca gaaggtccaa tacgtattcc acagcaagaa agtgttggtt taaataattt
     3601 gaccagtaca tcatcacaag gatcagaatc acggacaaac gcgccttata tccccattga
     3661 aacaacacca ataagtcgaa cagatgttgg gccgactcca atcgtatccg cgatgactga
     3721 tgctccttcg gttgaaatat taccaacgcc ccaaagaggg agaagcagaa aaattcctat
     3781 agtgtctcct aaaaatgtat ctgagtccat agttgctcct atcactgatg aaacggatga
     3841 tgcagggtca acaccgatat ccagagccac tgaagaatct atgccaccta atcagaccca
     3901 tccaaattta ctcatatccg attcatcaaa acataagcaa gcagtatcaa atagtgagat
     3961 ttcaaaagac gctcctactg gtttaaagga aatgcatgtt gctgagctgg agtccagtgt
     4021 agcctccgaa aatcatccag ctggaatact agatgtcaat gtcaacaaga gttcacaatc
     4081 attggatttt tctgcagatc ccgaaattga tagtattggc gcaatatcac ctacgagtcc
     4141 aaacgcagtt tctcctattt tacacgaagt tttagataat actgagttgt caaaaaaatt
     4201 agagaattct acgacagaaa gatttaaaga tagccaaagc gtagagaagc caactcatca
     4261 agaactaagt ttaagaaatg ccacagacga gactgaaata tccgcaatgg cattacaaga
     4321 tgtgaatagt ttggataata accaaattga aaaaacatac ccttcaaagc cgaaactcaa
     4381 tgtagatgtt tcagaagaca tttcctccag agagtcagca ataaagtcaa cttctacaaa
     4441 aaatacagga gtagatgtag gtctccaatc agattctaag ccagagacta cccttaacga
     4501 taaacaggac tctactgacc taaaagtgaa agtgtctgcg aaaatttcat ctatcataaa
     4561 ctataatgag ggtcaatggt caccaaacaa tccaagcgga aaaaaacaat acgaccgaga
     4621 acagttatta cagttacgcg aggttaaggc ctcacgtata caaccagagg taaaaaatgt
     4681 gtctattctt cctcaaccaa acttaatgcc atcatttatt cgaaataaca ataataacaa
     4741 gcgagtacaa tcgatggttg gtattattgg caatcgtagc aatgaatcgg cgggaaatta
     4801 tattggtaaa cagatgtcga tgtctggcgt acaaagtggc ggcggtagaa gcagtatgaa
     4861 gggcatgata cacgtaaatt tatcattaaa tcaagatgta aagttaagtg aaaatgaaaa
     4921 tgcatggcgc cctcgggtct taaataaatc tgatggtgat agcgacgcga aatctgcact
     4981 cgaaaaagat gaattggtac gtcgtgttcg gggtatttta aataagctta ccccagaaag
     5041 gtttgacaca cttgttgagg agattataaa attgaaaatt gatacacccg acaaagtgga
     5101 cgaagtaatt gtgttggtat ttgaaaaggc cattgatgag ccaaactttt ctgtgtctta
     5161 cgccagacta tgtcaacgac ttgctgcgga agtgaaggta atagacgagc gcatggaaag
     5221 cgaaaccaaa tctaactcgg cacactttcg aaatgcattg ttagataaaa ctgaacagga
     5281 attcacccaa aacgtttcac aaagcaccgc aaaggaaaaa aaattacagc caattgtgga
     5341 taaaattaag aaatgtactg atgccaatga aaaagcggaa cttgaggcat ttttggaaga
     5401 agaagaaaga aaaataagaa gacgatccgg agggacagta cgatttattg gcgaactttt
     5461 taagatatct atgttgactg gcaaaataat atactcttgc atcgatacac ttcttaaccc
     5521 tcattctgaa gacatgcttg agtgtttgtg caagctgctt acaactgtgg gtgctaaatt
     5581 tgaaaagacc ccagtaaatt ccaaagaccc tagtcggtgt tattcgttgg aaaaatcgat
     5641 caccaggatg caggccatcg catcgaaaac tgacaaagat ggagccagag taagctcaag
     5701 agtacgattt atgcttcaag acgttatcga cttgcgtaag aataaatggc aaacttcgcg
     5761 gaatgaagca cccaaaacta tgggacaaat tgaaaaagag gcaaagaatg agcagctttc
     5821 tgcacaatat tttggtacac tttccagtac tacacccgga ggatcgcagg gcggctctgg
     5881 taaacgagat gaccgtggaa actctcgata cggtgaatct cgttcaagta gcgcgtatgg
     5941 tggcagccac agtcaacgtg gtgataatgg caaccttcga caccagcagc agaacaacgt
     6001 cgggggaaac gtctctggag gtgctggaca ctccaatgga aacaatgatg aaaatacttg
     6061 gcatgtgcaa accagcaaag gtagccgctc tctagcggtt gacagcaata aactggaagg
     6121 cctgtctaaa ctttctgacc aaaatctgga aacaaagaag atgggtggat taacgcaatt
     6181 catttggatt agtagtgaca cgacaagact atcgtcggcg ccgacaccaa ccccatccaa
     6241 tcctttcgct gtgctctcat ccctaattga taaaaatagt aatgaaaggg atcgcgatcg
     6301 cagtggaccc agaaacaaag gatcatacaa caaaggttct atggagcggg atcgttatga
     6361 tagaggtatg cattcgagaa ctggatcatc tcagggatca cgagaaaact catcctcccg
     6421 cggtggtcag cagggtcgaa ctttattgag ttcatcagtt caaaaatcta ctagtcattc
     6481 aaaatacacc caacaggcac cacctacacg gcatactgtt aaagcccagt catcagtggg
     6541 cagctctaac gttaacacag gtccactcta ccgtggaagt gagcagcaaa catcagcaac
     6601 cttctcgcag accacccgtt ctgttgctcc tgtagcagtg ttcatcgaag ctagcgaaac
     6661 agatttaaaa ctcatcaaat ctgttgtttc agaaattgta gacctatcag ctgcatctaa
     6721 agaagtcacg cccggtgcag tttcgtgcat aaaacgagta ccagagaaac tacggtgcag
     6781 tttcatttat tatattttaa cagattattt gcatttagca aatgtaggta aacagtacag
     6841 gcggtattta tctattgccg tctcccaact tattcagcag aattacattt ctgccgatca
     6901 tttgagactg gcttacaacg aatttaccgt atatgcaaac gatttgattg ttgatattcc
     6961 ggagctttgg ctatacattc ttcaatttgc cgggccacta attgtaaaga aaatattgac
     7021 tatatcagat ttgtggaaca acaatttaaa agaaaatagt ccatcaaacg tcgctaaaaa
     7081 gttccttaaa acatacttga tatattgcac acaagaagtt ggaccaaatt ttgctcggaa
     7141 tatgtggatc aagtttaacc taaaatggtc agattttatg cctgaaagtg aagttgccga
     7201 ctttattaaa tttaacagac tggaatatgt ggaaaacgaa tcaaagtcgc cagtgattga
     7261 tcatagagag actccagaaa agcacgttaa aaatgtgata gaccatattg agcacttact
     7321 aaaggaagga acaacggcgg actgcattat tgattatagt aacggaaata taatggtggt
     7381 tgataagttg tttatcagag gattgacgga aacattgagt aatttctcga ttcactacaa
     7441 ggacaacagt tacaagcttg agtccgaaac ctttcaaaag ttttgcatac cggttttgca
     7501 gagatacatt gattctaatg aagaccacca acttgaatgt ttatatactt tgcagctttt
     7561 agttcatgga ttggagcatc caagagggtt gctgagtgaa ctaattggcg agctatatga
     7621 tgcctttgta atacagaagg aatcgctttg taagtggcga gattcaaagg atcagtctgc
     7681 tggcaagggc gttgctgtaa aaagtcttaa tccttttttt aactcgttgt taaacgatga
     7741 tgccaaccct ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     7801 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     7861 agcctcgact gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga
     7921 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     7981 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     8041 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     8101 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagtcgac
     8161 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     8221 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     8281 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     8341 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     8401 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     8461 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     8521 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     8581 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagccagcaa aaggccagga
     8641 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8701 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8761 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8821 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     8881 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8941 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9001 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9061 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9121 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9181 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9241 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9301 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     9361 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9421 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9481 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9541 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9601 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9661 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9721 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9781 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9841 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9901 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9961 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    10021 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
    10081 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    10141 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    10201 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    10261 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    10321 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    10381 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
    10441 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgt
//
