LOCUS       sequence-289030-        7743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmDCP2-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Nishihara T, Zekri L, Braun JE, Izaurralde E
  TITLE     miRISC recruits decapping factors to miRNA targets to enhance their 
            degradation.
  JOURNAL   Nucleic Acids Res. 2013 Oct;41(18):8692-705. doi: 
            10.1093/nar/gkt619. Epub 2013 Jul 17.
  PUBMED    23863838
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkt619"; journalName: "Nucleic Acids Res"; date: 
            "2013-10"; volume: "41"; issue: "18"; pages: "8692-705"
COMMENT     Sequence Label: pAc5.1A-DmDCP2-dsRNAres-V5His6_I
FEATURES             Location/Qualifiers
     source          1..7743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             5028..5069
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5079..5096
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5119..5136)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5169..5303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5193..5212)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5247..5266
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5925..6513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6005..6024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6684..7475,7476..7544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7307..7326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7545..7649)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7717..7735
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctactag tccagtgtgg tggaattctg cagataagtg atatcccaac atggagattg
     2641 ccttgttgat taacaatgct gtggccgtgg tgaccgcttc ggctagtgct gctgtgtcgg
     2701 ctagtgcctc cgtgggatcc tcctccaaag acgacaatgt gaataacctc gccctgctgg
     2761 ccagcttgac cctacagaag gtcctgaaca ttaataacaa taacaatcaa cattccaaaa
     2821 ataataatca ttccgataac tccaactcca agcagcatga acagcagaag caacaacaac
     2881 agccccagcg ccgcaatctg gatatcaagt ccgaatacga cgttttgaat cagctgttga
     2941 agaccggcgc cgtgtttacg gctaccccga ccgcccccat cgctattgag cgccgcaaac
     3001 aaacccaaca acaacagcgc ccctacgccc agcaagccca ccagcaacaa caacaaaaac
     3061 agcgtctgaa ttcgagcatt tccagcacgc attccagcac cagcaccaat agcagctatg
     3121 gatcctcgtc gagcgaagac gcagctggaa cttcggctcc cactggtgct ggagaggtgg
     3181 ttacttccgc tgctctaact ccccgagctt ccacaacaaa agctagctcc aacaagctgc
     3241 ccgaaaagag caagatcccc agcgacattc tggacgactt ggcttcccgc ttcattatta
     3301 acgtgcccga tatggaactg aataacctca tacgtatgtg tttccaaatc gaactggccc
     3361 attggttcta tttggatttc ttttgtgccc ccgaatctgg cgaggatggc gagaccccga
     3421 agtgcgtgca acgaaagctg ccatcggtgg gcatcaagca gtttgccatg cagttgttcc
     3481 aacacattcc cttcctgaac aaacattttg gcaccgtgga tcaaatcctg gacgagtgga
     3541 agaactacaa gctatcggtg cccacttatg gcgctattct ggtgtccgag gatcacaatc
     3601 actgcttgct agtgcagtcc tacttcgcgc gcaactcctg gggatttccc aagggcaaga
     3661 tcaatgagaa cgaagaccca gcccattgtg caacgagaga ggtttatgag gagaccgggt
     3721 tcgatatcac ggacctaatc gatgccaacg actacatcga ggcgttcatc aactaccagt
     3781 acacgcggct gtacgtggtg cgaaacatac cgatggacac gcagttcgcg ccccgcactc
     3841 gcaacgagat caagtgctgc gactggttcc gtatcgatgc gctgccggtg aacaagaacg
     3901 atgccatatc aaaggccaag ttgggcaaga cctccaactc cttcttcatg attatgccgt
     3961 ttgtgaagcg cctgaagaag tgggtgaacg atcggaaggc tggaatcgag ccgcgtcgcc
     4021 gcaaattctc tggtcagcaa tcgccaaaag cggcctcgcc caccaatatg aacggaaatt
     4081 gcaagaagga cgtcacagga gtgcgaaatg aatcgcaacg acagaggcac aagtccatgg
     4141 gcgacttgga tggcgtcaaa ttgaataatc tcaatggcaa ggggaccgtt ggaacgggag
     4201 cgggatcggc agcttccgcc tccgccaccg ctgccatcaa ctcgatgaac atatgcaata
     4261 gcaatgggaa acgcaataag caaaatgcgg ctgcgggcag caatcaggca acgccaacta
     4321 caatagcaac gccaatgacg tcaaatggcg ccgtcgtagg aggtactgta tcctcgaagc
     4381 gtcaactgtt ccatagccaa agtcaggacg atgcgcagca atcggcaagc aatgagaaga
     4441 ttgtcagctc cttcgattta atacgcaagg agaagcagca acaacttaag gcagctaagg
     4501 cgcaaaagca gcagcaacaa cagcagcaac gatcgcgcac caagtcgcag agtgataagc
     4561 agtacaatcc acagcagcca gtcaaaatcc tgggacaaca gcagcccaca acgggcatgg
     4621 atttgatagc catgctatcg gcagcggcgg cagctcaaaa gacaccaaac aacgagcagc
     4681 aaacacaaga gaagcagcag cagcaacaac gaccacgacc tgcttcagtg tcgcttaacc
     4741 ttggagcagg cagtgccaca ggagcaacaa catcagctcc ggatgcccag tacatgcaca
     4801 ggctgcagcg catggcatcg ggcacaaatt tgaggattct gaagcgggca cagtcgcagc
     4861 agccgcagca gcaccatcag caacagttgc aactagaaca gcaacaacag cagcagcagc
     4921 agctgggaat cgactttacg ccaaatttga attcgtggac gaatttctcc tttaccaaga
     4981 acttcatagc aaatgtgttt tgcctcgagt ctagagggcc cttcgaaggt aagcctatcc
     5041 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     5101 tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct
     5161 cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     5221 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     5281 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     5341 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     5401 attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     5461 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     5521 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     5581 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     5641 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     5701 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     5761 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     5821 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     5881 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     6121 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6361 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6541 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6601 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6661 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6721 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6781 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6841 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6901 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6961 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     7021 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7081 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7141 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7201 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7261 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     7321 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7381 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7441 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7501 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7561 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7621 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7681 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     7741 cgt
//
