LOCUS       sequence-289031-        8459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmDCP2-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8459)
  AUTHORS   Nishihara T, Zekri L, Braun JE, Izaurralde E
  TITLE     miRISC recruits decapping factors to miRNA targets to enhance their 
            degradation.
  JOURNAL   Nucleic Acids Res. 2013 Oct;41(18):8692-705. doi: 
            10.1093/nar/gkt619. Epub 2013 Jul 17.
  PUBMED    23863838
REFERENCE   2  (bases 1 to 8459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkt619"; journalName: "Nucleic Acids Res"; date: 
            "2013-10"; volume: "41"; issue: "18"; pages: "8692-705"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmDCP2-dsRNAres-V5His6_I
FEATURES             Location/Qualifiers
     source          1..8459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5744..5785
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5795..5812
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5835..5852)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5885..6019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5909..5928)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5963..5982
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6641..7229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6721..6740)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7400..8191,8192..8260))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8023..8042
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8261..8365)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8433..8451
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcca gcacagtggc ggccgcatgg agattgcctt
     3361 gttgattaac aatgctgtgg ccgtggtgac cgcttcggct agtgctgctg tgtcggctag
     3421 tgcctccgtg ggatcctcct ccaaagacga caatgtgaat aacctcgccc tgctggccag
     3481 cttgacccta cagaaggtcc tgaacattaa taacaataac aatcaacatt ccaaaaataa
     3541 taatcattcc gataactcca actccaagca gcatgaacag cagaagcaac aacaacagcc
     3601 ccagcgccgc aatctggata tcaagtccga atacgacgtt ttgaatcagc tgttgaagac
     3661 cggcgccgtg tttacggcta ccccgaccgc ccccatcgct attgagcgcc gcaaacaaac
     3721 ccaacaacaa cagcgcccct acgcccagca agcccaccag caacaacaac aaaaacagcg
     3781 tctgaattcg agcatttcca gcacgcattc cagcaccagc accaatagca gctatggatc
     3841 ctcgtcgagc gaagacgcag ctggaacttc ggctcccact ggtgctggag aggtggttac
     3901 ttccgctgct ctaactcccc gagcttccac aacaaaagct agctccaaca agctgcccga
     3961 aaagagcaag atccccagcg acattctgga cgacttggct tcccgcttca ttattaacgt
     4021 gcccgatatg gaactgaata acctcatacg tatgtgtttc caaatcgaac tggcccattg
     4081 gttctatttg gatttctttt gtgcccccga atctggcgag gatggcgaga ccccgaagtg
     4141 cgtgcaacga aagctgccat cggtgggcat caagcagttt gccatgcagt tgttccaaca
     4201 cattcccttc ctgaacaaac attttggcac cgtggatcaa atcctggacg agtggaagaa
     4261 ctacaagcta tcggtgccca cttatggcgc tattctggtg tccgaggatc acaatcactg
     4321 cttgctagtg cagtcctact tcgcgcgcaa ctcctgggga tttcccaagg gcaagatcaa
     4381 tgagaacgaa gacccagccc attgtgcaac gagagaggtt tatgaggaga ccgggttcga
     4441 tatcacggac ctaatcgatg ccaacgacta catcgaggcg ttcatcaact accagtacac
     4501 gcggctgtac gtggtgcgaa acataccgat ggacacgcag ttcgcgcccc gcactcgcaa
     4561 cgagatcaag tgctgcgact ggttccgtat cgatgcgctg ccggtgaaca agaacgatgc
     4621 catatcaaag gccaagttgg gcaagacctc caactccttc ttcatgatta tgccgtttgt
     4681 gaagcgcctg aagaagtggg tgaacgatcg gaaggctgga atcgagccgc gtcgccgcaa
     4741 attctctggt cagcaatcgc caaaagcggc ctcgcccacc aatatgaacg gaaattgcaa
     4801 gaaggacgtc acaggagtgc gaaatgaatc gcaacgacag aggcacaagt ccatgggcga
     4861 cttggatggc gtcaaattga ataatctcaa tggcaagggg accgttggaa cgggagcggg
     4921 atcggcagct tccgcctccg ccaccgctgc catcaactcg atgaacatat gcaatagcaa
     4981 tgggaaacgc aataagcaaa atgcggctgc gggcagcaat caggcaacgc caactacaat
     5041 agcaacgcca atgacgtcaa atggcgccgt cgtaggaggt actgtatcct cgaagcgtca
     5101 actgttccat agccaaagtc aggacgatgc gcagcaatcg gcaagcaatg agaagattgt
     5161 cagctccttc gatttaatac gcaaggagaa gcagcaacaa cttaaggcag ctaaggcgca
     5221 aaagcagcag caacaacagc agcaacgatc gcgcaccaag tcgcagagtg ataagcagta
     5281 caatccacag cagccagtca aaatcctggg acaacagcag cccacaacgg gcatggattt
     5341 gatagccatg ctatcggcag cggcggcagc tcaaaagaca ccaaacaacg agcagcaaac
     5401 acaagagaag cagcagcagc aacaacgacc acgacctgct tcagtgtcgc ttaaccttgg
     5461 agcaggcagt gccacaggag caacaacatc agctccggat gcccagtaca tgcacaggct
     5521 gcagcgcatg gcatcgggca caaatttgag gattctgaag cgggcacagt cgcagcagcc
     5581 gcagcagcac catcagcaac agttgcaact agaacagcaa caacagcagc agcagcagct
     5641 gggaatcgac tttacgccaa atttgaattc gtggacgaat ttctccttta ccaagaactt
     5701 catagccaat gtgttttgca gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     5761 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
     5821 aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat
     5881 cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     5941 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6001 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6061 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6121 tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     6181 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     6241 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     6301 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     6361 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     6421 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     6481 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     6541 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagccagc
     6601 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6661 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6721 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6781 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     6841 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6901 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6961 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7021 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7081 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7141 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7201 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7261 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7321 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7381 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7441 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7501 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7561 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7621 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7681 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     7741 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7801 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7861 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7921 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7981 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     8041 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8101 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8161 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8221 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8281 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8341 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8401 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgt
//
