LOCUS       sequence-289045-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delMID2-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delMID2-siRNAres_I
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3530..3547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6166..6287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6177..6196)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6231..6250
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6473..6928
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6560..6579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6770..6791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7045..7402
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7045..7236
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7068..7088)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7253..7388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7453..8247
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7507..7526)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8117..8136
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8311..8359
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8400..8419)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8519..8541
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8579..8597)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8665..8769
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8770..8838,8839..9630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8988..9007)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9801..10389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10290..10309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcttt gagcgccaag cagaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccagt tgccaaggaa tgatcttgac cctagggtcc
     2581 tctccaacac tggttgggga cagactcctg taaagcaaaa cactgcctgg gaatttgaag
     2641 aatcccctag gtctgaaagg aaaaatgaca atgggacaga ggcctggggt tgtgcagcta
     2701 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2761 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2821 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2881 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2941 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     3001 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     3061 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3121 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3181 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3241 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3301 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3361 gcagtgaagc aactggctgg gaagaaccct ctccaccgtc cattcgccgc aaaatggaaa
     3421 ttgatgatgg tacctcagct tggggggacc caagcaacta taacaataaa actgtaaaca
     3481 tgtgggatag aaacaacccg gtcatccaga gcagtaccac gaccaatacc accaccacca
     3541 ccaccactac cacgagcaac accacacaca gggtcgagac gccgcccccg caccaggctg
     3601 gtactcagct gaatcgatca ccgttgcttg gtccaggtag gaaagtttca tcaggctggg
     3661 gagaaatgcc taatgttcac tcaaagactg aaaactcttg gggagaacca tcctcccctt
     3721 ctaccctggt ggataatggc acagcagcat gggggaagcc acccagcagt ggcagcgggt
     3781 ggggagatca ccctgccgag ccgccggtgg catttggaag agctggcgca cctgttgctg
     3841 cctcagccct gtgcaaacca gcttcaaaat ctatgcaaga aggctggggc agtggtgggg
     3901 atgaaatgaa cctcagtacc agccagtggg aggatgaaga aggggacgtg tggaataatg
     3961 ctgcttccca agaaagcacc tcctcctgca gctcctgggg gaacgccccc aaaaaaggac
     4021 ttcaaaaggg catgaagacg tctggcaagc aggatgaggc ctggatcatg agccggctga
     4081 tcaaacaact cacagacatg ggcttcccga gagagccagc tgaggaggcc ttgaagagta
     4141 acaatatgaa tcttgatcag gccatgagcg ctctgctgga aaagaaggtg gacgtggaca
     4201 agcgtgggct gggagtgacc gaccataatg gaatggccgc caagcccctc ggctgccgcc
     4261 cgccaatctc caaagagtct tccgtggacc gccccacctt tcttgacaag gatggcggcc
     4321 tcgtggaaga gcccacgcct tcaccgttct tgccttcccc aagcctgaag ctcccccttt
     4381 cacacagtgc actccccagt caggccctgg gtgggattgc ctccgggctg ggcatgcaaa
     4441 acttgaattc ttctagacag ataccgagtg gcaatctggg tatgtttggc aatagtggag
     4501 cagcacaagc caggaccatg cagcagccgc cacagccacc agtgcagcct cttaactctt
     4561 cccagcccag tctccgtgct caagtgcctc agtttctatc ccctcaggtt caagcacagc
     4621 ttttgcagtt tgcagcaaaa aacattggtc tcaaccctgc actattaacc tcgccaatta
     4681 atcctcaaca tatgacgatg ttgaaccagc tctatcagct gcagctggca taccaacgtt
     4741 tacaaatcca gcagcagatg ttacaggccc agcgtaatgt gtccggatcc atgagacaac
     4801 aggagcagca agttgcgcgc acaatcacta atctgcagca gcagatccag cagcaccagc
     4861 gccagctggc ccaggccctg ctcgtgaagc agccaccacc gccaccgccc ccgccgcacc
     4921 tgtctctgca cccctctgca ggcaaatcgg ccatggacag cttcccctcg cacccacaga
     4981 ctcccggcct acctgacctg cagaccaaag agcagcagtc ttcacccaac acctttgctc
     5041 cttaccctct cgctggactg aacccaaaca tgaatgtcaa cagcatggac atgaccggtg
     5101 gcttgtcggt gaaggaccca tcccagtccc agtcacgcct cccccagtgg acgcacccca
     5161 actccatgga taacttgccc agtgccgctt cccccctgga gcagaaccct agcaagcatg
     5221 gtgctatccc tggaggtcta agcattgggc ctccaggtaa gtcctccatt gatgactcct
     5281 atggccggta cgatttaatc cagaacagtg agtcaccagc cagtcctccc gtagctgttc
     5341 cccatagctg gtcacgtgcc aaatctgaca gtgataaaat ctcaaatggc tctagcatca
     5401 actggccccc agaattccat ccgggagttc catggaaagg actgcagaat attgaccctg
     5461 agaatgaccc tgacgtcact cctggcagta gaaccagcag ctggctcgtt cttcgaaacc
     5521 tcactcccca gattgatggt tctacactgc ggacattgtg tttgcaacat gggcctctta
     5581 tcacattcca cctgaatctg actcaaggca atgctgtggt ccggtacagc tccaaggagg
     5641 aggctgccaa ggcccagaag tctctgcaca tgtgcgtcct gggaaacact accatcctgg
     5701 ccgagttcgc tggtgaagaa gaagtgaatc gcttcttagc ccaaggccag gcgctgccac
     5761 ccacttccag ctggcagtcc agcagcgcgt ccagccagcc gcggctcagc gcagcgggca
     5821 gctcccatgg cctggtacgc agcgacgctg gccactggaa cgccccgtgc ctgggtggca
     5881 aggggagcag tgagctgctg tggggcgggg tgccccagta ctccagcagc ctgtggggcc
     5941 cgcccagcgc cgacgacagc agggtgatag gcagccccac gccgctaacc accctgctgc
     6001 ctggggacct gctcagcggg gagtccctgt aggcgctgcc atcatcagca ccaggagagc
     6061 cgacccctcc cgggacccct cccggctggg cggccccaca gacccgctgg aacccagcag
     6121 cggccgcttc cctttagtga gggttaatgc ttcgagcaga catgataaga tacattgatg
     6181 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     6241 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     6301 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa
     6361 acctctacaa atgtggtaaa atccgataag gatcgatccg ggctggcgta atagcgaaga
     6421 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     6481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6901 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     6961 gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga
     7021 aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca
     7081 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7141 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7201 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7261 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7321 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7381 tggaggccta ggcttttgca aaaagcttga ttcttctgac acaacagtct cgaacttaag
     7441 gctagagcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7501 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7561 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7621 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     7681 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     7741 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     7801 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     7861 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     7921 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     7981 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8041 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8101 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8161 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8221 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     8281 cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc attacatctg
     8341 tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc gcgtatggtg cactctcagt
     8401 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     8461 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8521 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     8581 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8641 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8701 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8761 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8821 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8881 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8941 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9001 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9061 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9121 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9181 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9241 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9301 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9361 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9421 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9481 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9541 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9601 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9661 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9721 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9781 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9841 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9901 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9961 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10021 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10081 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10141 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10201 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10261 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10321 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10381 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10441 gctcacatgg ctcgacagat ct
//
