LOCUS       sequence-28905-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Lomenick B, Hao R, Jonai N, Chin RM, Aghajan M, Warburton S, Wang J,
            Wu RP, Gomez F, Loo JA, Wohlschlegel JA, Vondriska TM, Pelletier J, 
            Herschman HR, Clardy J, Clarke CF, Huang J
  TITLE     Target identification using drug affinity responsive target 
            stability (DARTS).
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21984-9. doi: 
            10.1073/pnas.0910040106. Epub 2009 Dec 7.
  PUBMED    19995983
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0910040106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-12-22- 22"; volume: "106"; issue: "51"; pages: "21984-9"
COMMENT     Sequence Label: pcDNA FLAG mTOR ST
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             232..510
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEATRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
ORIGIN
        1 ttgcagattg atacgcccag acccttggtg ggacgtctca ttcaccagct tctcacagac
       61 attggtcggt accaccccca ggccctcatc tacccactga cagtggcttc taagtctacc
      121 acgacagccc ggcacaatgc agccaacaag attctgaaga acatgtgtga gcacagcaac
      181 accctggtcc agcaggccat gatggtgagc gaggagctga tccgagtggc catcctctgg
      241 catgagatgt ggcatgaagg cctggaagag gcaactcgtt tgtactttgg ggaaaggaac
      301 gtgaaaggca tgtttgaggt gctggagccc ttgcatgcta tgatggaacg gggcccccag
      361 actctgaagg aaacatcctt taatcaggcc tatggtcgag atttaatgga ggcccaagag
      421 tggtgcagga agtacatgaa atcagggaat gtcaaggacc tcacccaagc ctgggacctc
      481 tattatcatg tgttccgacg aatctcaaag cagctgcctc agctcacatc cttagagctg
      541 caatatgttt ccccaaaact tctgatgtgc cgggaccttg aattggctgt gccaggaaca
      601 tatgacccca accagccaat cattcgcatt cagtccatag caccgtcttt gcaagtcatc
      661 acatccaagc agaggccccg ggaaattgac acttatgggc agcaacggac atgagtttgt
      721 t
//
