LOCUS       sequence-289052-       10140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1147-2505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot1_1147-2505_I
FEATURES             Location/Qualifiers
     source          1..10140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7425..7466
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7476..7493
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7516..7533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7566..7700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7590..7609)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7644..7663
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8322..8910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8402..8421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9081..9872,9873..9941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9704..9723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9942..10046)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10114..10132
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttaattaaga tggtgactgt gccaccagag cctgtgcagg acaaaactgc
     3361 ctttattttc aacaacttga gccagctgaa cataccgcag aagtgcgatg agataaagga
     3421 gattatgacc aaggagtatt ggccgtggct agcgcaatat ctggtcctca aacgcgcatc
     3481 aatggagttc aacttccaca cactatatta caactttttg gatgccctta aaaacggcga
     3541 gatcaaccga ttcgtaacca aagagaccct acgcaacatc aaggtactct tgcgctccga
     3601 taagggagtt attaatttct ccgatcgaag tctgttgaag aacctcggac actggttggg
     3661 catgatgacc ttaggtcgca atcgccccat cctgcagttg gatctggatc ttaaatccct
     3721 tttggctgag gcttaccaca agggccagca ggaactgctt tttgtggttc ctttcgtagc
     3781 caagatcctt gagtcatccg ccaagtctcg catcttccga tcgcccaatc catggacaat
     3841 gggtatcatg tacgtgcttg ctgagcttca ccaggagcca gaccttaagc tgaacctgaa
     3901 gtttgaaatc gaagtactct gcaaaacact taatctggag ttggccaagt tgcgacaagt
     3961 gatctacctg aaggatccca atcgaactca ccggattgaa gagcagatgt cgcaaccaaa
     4021 accgaaacaa cttgagccag tggcttctgc gccagctcta ccgcgtgaac agcaatcccc
     4081 ggcacaacct ccgccaccac cacaacagca gcaaccaccg cagcagcagg tacctccacc
     4141 gccatcctct gcggacgtgg acgcccaaaa tgcggccgct atgatgatgg cagcgggcgg
     4201 agctaatagt actcctggat cggtatcttc gcccaatcta cccaccgatt ccagccaggt
     4261 ggcactaccg ccaccagagc cgcgctattc atacgtggac gtaaacgtga gcaacttcca
     4321 gcttattggt cagcagttgg tactgccacc caatactccg ttcctgcacg ccaatcccgg
     4381 aatcaaacac atggtcgtca acgccgtgga gcgcacgata accgactggt tgcagcctat
     4441 tgtggaccga agcatccgca ttgcctgtgc caccaccgag cagatcatcc gcaaggactt
     4501 cgccctggat gctgacgaga accgaatgcg cactgccgct catcagatgg tacgaaacct
     4561 tgccgccggc atggccatga ttaccggtaa ggatgagata gcgcgtgcta ttagccagaa
     4621 tctgcacaag gccttgctgt cgggtctaaa tgggatgccc agcatggctg agatccaagc
     4681 tgctgctatg cagttagcca gcgaaaatgt tgagctagtt tgcgctttca ttcagaaaac
     4741 atctgctgaa aaggcagccg cagagatcga taggcgtcta agcaccgatt tcgagaccag
     4801 aaaaattgct cgtgaagagg gcaaccgctt tgtggacgcc cagatcctca cctatcaaca
     4861 ggagcgtctt ccagaagcag tgcgcatcaa ggtgggcgcg gctccagcta cactttatgc
     4921 tgtgtactcg gagttcgcta gaagcatacc tggcttccag cagatgagcg atcgcgatat
     4981 tgccctgttt gtgcccaagc cgacggattt gtctcagcca aatgtgtttg ctaatgatga
     5041 tagcatggta tacggggaac tggctagcaa aatggaggcc ttcatgaaca cggcaatcgg
     5101 agtgccgaca ctccagatcc aggctagcaa gatgcacatg ctactcaacg ccctgatagc
     5161 gacgcgtcga ctgcgtgacc aagagtctgc ctttaatctg ctgacccgcg cggtcgaggg
     5221 tttgaccgaa ggattggtca atatgcatga aaacatggag caaatgaaga tgtaccaaaa
     5281 catccatctc cgtatccttg gcttgctgaa caacagtttc ggtgctccaa acacagagcg
     5341 agcggtaaca aagtgtttct tcgatatccg agaggaggtg cgatataacg tggaggcagc
     5401 tcgcgctttg attacgtcgc actttgtcaa tctgaatcag tttgacggaa tgctacgcga
     5461 ctgcatggac aacggaaaca attatgtggc catatcgttc ggtatcgcac tgctggagcg
     5521 cctcatcatg gacgatcgag tgattaatat tgtttcggac aacgaattca tggcaactgt
     5581 tgagctgcta ggtaggctca ctcagcatcg ccatcggtat ccagaatgca ttgtgaatgc
     5641 tattgatacg ctctggagcg gaaactttaa ctcgagcagc gactacagcc cgttcaacgg
     5701 caatgatcgt tacttgtcgg gagcctccca ttacatccac tccggcatgc atcacgtaag
     5761 gtcatgcgat acagatgatc cgccgggctt acaagagaag acggagtttc tgcttaagga
     5821 ttgggttgca ctgtacacgc aacagaacca gcagtcaacg cgcgatgcac gcaacttcag
     5881 tgcatttgtt cagaagatga acacatatgg tatcctgaaa acggacgact tgatcacccg
     5941 ttttttccgc caggccacac acatttgcac ggatgtggtg tacagaatgt tcgccgagcc
     6001 gagtttacca atcaaccagg ccaaaaacaa gatattccag tggatcgatg cgttcgtcca
     6061 tctgattgca atgctcgtgc gacactcagg cgaagcaggt aatccaacca ccaagatcaa
     6121 cctgttaaac aaggtgctgg gcattgtgct gggaacgctg attaaggacc atgagatgcg
     6181 tggcgttagc ttccagcagg ttggctacca tcgcttcttc atgatgctgt tcatggagct
     6241 gtgcacggcc gatgtgattc tcgagtccct gatgcacagt attgtgtcgg ccttcgccta
     6301 cacatatcac ttgttgaatc ccagcgtggc tccgggattc tgctttgctt ggttggagct
     6361 catttcgcat cgcgtcttcc tgggccgcat cctggtccaa attcccgggc aaaagggctg
     6421 gccactttac gctcagttgc tgcaggacct ttttaaatat cttgcgccct tcttacgcaa
     6481 cactgagctc ggcaaaccag tccagctttt gtacaagggg acgctacgtg ttctgctcgt
     6541 tctgcttcac gacttcccgg agttcttgtg cgactatcat tttgggttct gtgacaccat
     6601 cccgcccaat tgcgtccaga tgcgtaacat catcctgtcg gcgtttccgc gcaacatgcg
     6661 cttgcccgat cccttcacgc ccaacctaaa agtggacatg ctgtcggaca gtagtaatgc
     6721 gcctaaggtg ctcagtagct acattatgaa catccagccc gcgaacttta agaaggattt
     6781 ggactcgtac ctcaaggccc gggcgccagt tacttttcta tcggaattac gtggtcactt
     6841 gcaggtgaca agcgagccag gaactcgtta caacatggcg ctgatgaatg ctctggtcat
     6901 gtatgtggga actcaggcga ttgctttgat cagaaacaaa aactttgtgc ccaacacctc
     6961 gaacatagca cacagcgccc acatggatat cttccagaat cttgctgttg acttggatac
     7021 ggagggtcgc tatctgttct tgaatgcgat tgcgaaccag ttgcgctatc ccaacagcca
     7081 cacgcactac ttcagctgtg cggtgctgca tctgtttgcc gaggccaact cggaggcaat
     7141 tcaggagcag atcactcgcg tacttctgga acgattgatc gtgaaccgcc cgcatccttg
     7201 gggactgttg atcactttta ttgagctgat caaaaatccc atctacaagt tctgggacca
     7261 tgactttgtg cactgtgcgc cagagatcac caagctcttt gaatcggtgg cccgttcctg
     7321 tctggccaag tcgaacgtca cccagcagtt gaacatgccc gtcgtcgatg gcgagggcca
     7381 ggaggtagcc accatcaact gatctagagg gcccgcggtt cgaaggtaag cctatcccta
     7441 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
     7501 aaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga
     7561 tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     7621 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     7681 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     7741 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     7801 atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     7861 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     7921 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     7981 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     8041 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     8101 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     8161 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     8221 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccag
     8281 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8341 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8401 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8461 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     8521 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8581 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8641 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8701 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8761 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8821 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8881 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8941 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9001 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9061 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9121 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9181 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9241 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9301 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9361 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     9421 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9481 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9541 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9601 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9661 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     9721 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9781 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9841 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9901 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    10081 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
