LOCUS       sequence-289059-        8916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1-1148.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1_1-1148_I
FEATURES             Location/Qualifiers
     source          1..8916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6201..6242
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6252..6269
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6292..6309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6342..6476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6366..6385)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6420..6439
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7098..7686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7178..7197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7857..8648,8649..8717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8480..8499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8718..8822)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8890..8908
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt cattaattaa gatgaacgta gagagccaac tgaagacacc
     2761 gccaacgcac attcgaaatc tggtcaagca cgtgaacaag cgaaatttta atgagagcag
     2821 cgagcaaata aatcagttcg ttaaagagca cggcctggaa gcggatcgca gcagcctgcg
     2881 ccacctgttc tccgtgatca acttcagcga tctcgtgcca tcagttacgg tccagctgca
     2941 ggcgaagctc ctgggcatcc agttggagcg tcagcttcac agttcatcgt ttgtgtccaa
     3001 catctgctac gcttttgacc agttcttcgc cagcaaccag aagtccctga agcctgttgc
     3061 ggtagcggat ctcgttggcc aggtcgcccg actgaccggc atcaacaagg tttgtgagtg
     3121 cgtctttgcc ttggccttga cccactcctc gtacacagaa ctcaaacatt ctgccaggaa
     3181 caacctaaaa ggtagcctca gcgaacttat agactcttac ttgggtaata aaggcaccgg
     3241 cccagccgac agcggtctcc gggagatctc cttcgatcta ctgcagtatt tgctgtgctg
     3301 ccttagcgag tacgtacaac cgcaggtgga agcacagttt ttgatcaagc ttcgtgagga
     3361 gtttccgcgc caggcggtac cgctggttct agcgccgttc ctttacggct cgacgacggc
     3421 gacgattgcg ggagcaggtg ccagcgaaac ggatgcggag gccgaagcga cgactaatag
     3481 caacagctcc agtttcgaag cagatgcttt gaacgaggtg ggaatcgagg atatttatga
     3541 ccatttaagc gagataatat tcaccaacca gggcaaaaat aatattatgg acacatcttg
     3601 gattaatcta atccttgaaa tcggttatga atttacatcg agcgttgaag agtgtaagaa
     3661 tcatttgtgt tcccgcgagc gagaacgcgc tgagctccag tccaaagatg tcgccaagat
     3721 cgtgggtctt atgtgccgac ggcactcctc cctgctcgat tgtaatgtga atctaccgac
     3781 gccggcgaac ttctggcctg gtcagggaca gggcggtggc agcaacagca gtggctcttc
     3841 gcagacacaa atcactccgc agcaacagaa cccgggcagc agtaacaaca acgatggcag
     3901 cgatggcaat tcgtccagtg ataagaagga caagaaggag acgacggagg ccacgcaaac
     3961 ttggaagcca gatgtctttg tgcaggcgct caaggaggtg gtgcctcagc tcaactggaa
     4021 ggacgtgtgc atggaacttg atcatcccga gttcgtgctg aaggatcgca tcggtttgga
     4081 gcttttgctc accatccttc ggctggccac tggctctaac atatttccac atccagaatg
     4141 catttaccgt cactgggcaa acacggaagg tcagctgtcg cttatcgcga caatgctgaa
     4201 gaacccggat ctcttttcct tcgccgactt tgtatttagc cagccagcgt tggatgtgct
     4261 taagacagct ccggatgcgg acaataagga gatctcgtcc tggaagtcgc ttcacctggt
     4321 ggaggtgctg ctctccattg cggacaaggg atactatacg caggtccatg agctattcaa
     4381 gttcccagcg cagaactgtc ccgatgtgct atttttggcc ttattgaaca caagtccgcc
     4441 tatgacgcca ctgcgtcagg atctgtttaa ccaactgata ccaacgttcc ttggcaacca
     4501 tcccaactcg aacgtgatct tggccagcgc ctggagctcg aacagcttcc aacttcgttc
     4561 caatatcatg aatgctatgt ccgagtggta tctccgaggc aatgagttcg atcaggtgaa
     4621 actgtcgcgc atcctggatc ttgcccagga tttaaaagcg ctatcagcgc tgctcaacgc
     4681 ccgttcattt ttattcatta tcgacctggc ttgccttgcc tctcggcgcg agtacctgaa
     4741 gctggaaaag tggcttaccg ataaaattcg cgaacatgga gagcctttta tgcaggctat
     4801 aatcaaggtt ctgcaccgcc gctgcccgca ggttatcaac gccaaagtgc cagaggatca
     4861 gctgcctccc aagcaggcgc agcttttgcc cgagacagtt acaaccatga tcaactgtct
     4921 gcaaacgtgc atcaataact gcatgcagcc ggagatggtc gaagtcatca tgcaaatgac
     4981 cgcgaacgtg gcgattatgg ctaacatggc gcgtgcccaa cagcagcagc agccaggatt
     5041 ggttccgccg cctccaccaa caattctgcg cggccatcgt ggtatggatc tgcctggagg
     5101 catagtccct ccgccaccgc agcaaccgtt ctccggaaac ctcaacgcgc agatgtttgg
     5161 accaggaatg gatccgctaa cgaatatgtc caacaactta gccggcctca acctcagcgg
     5221 cccgaacgga gcatttaatt ttggaaacat gctaacttct ccatcgcgtc tgatgactcc
     5281 gggagccaat ccctatccgc tgaacctcat gcagatgccg caggctccgc caccacccaa
     5341 cgtaggaaac ctaggccgca tgctgccagg gggtccacaa cagcagactc caacgccgac
     5401 tccaacggcc cccaatccaa acaaccctgt gatggccgat ctccagatac ctgtgtcaaa
     5461 ggaagttgaa gacgaggtca actcatactt tcagcgcatc tacaaccacc agccgaatcc
     5521 aacgctgtcc atcgatgaag tgctggacat tttgcaaaga tttaaagagt caagtaaccg
     5581 gcgcgagcag gaagtctttt tgtgcatgct gcgcaatctg ttcgaggaat atcgcttttt
     5641 ctgtcaatac ccagagaaag agctacagat tactgcgcag ctatttggtg gcattattga
     5701 tcgtaacctg gtgcccactt ttgttgcact gggcctttct ctgcgctgtg tcttggatgc
     5761 cctccgcaag cccgatggat ctaagcttta ctatttcggt gtgacggcac tggacagatt
     5821 cagaactcga ttgcacacct acaacaaata ctgcgagcat atccgctcca ttcctcactt
     5881 ctctgacttc ccgccgcacc ttattcagta tgtggaatac ggaatgcacg gccaggagcc
     5941 gccgccgcag aagttgattg gactcagcaa tacgattcct tcggcgatat ctagcggacc
     6001 aggaacggaa ccaatttaca ggaatagctc catgttaggt aatatgcccg ctgccacccc
     6061 gggatcggga ccgaaatcaa acgctgctgt atcgcatgcc acgagaatga agtccatcgc
     6121 caatgccacc aatatcgaca cacttttggt ggccaaccag gaagagaaag tgacttgatc
     6181 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     6241 cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
     6301 tgccttctaa ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat
     6361 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     6421 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     6481 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     6541 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg
     6601 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     6661 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     6721 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     6781 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     6841 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     6901 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     6961 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     7021 cagcttcaag gatcgctcgc ggctcttacc agccagcaaa aggccaggaa ccgtaaaaag
     7081 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7141 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7261 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     7321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7501 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7561 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7621 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7681 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7741 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7801 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7861 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7921 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7981 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8041 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8101 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8161 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8221 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8281 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8341 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8401 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8461 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8521 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8581 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8641 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8701 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8761 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8821 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8881 acctataaaa ataggcgtat cacgaggccc tttcgt
//
