LOCUS       sequence-289061-        9543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1147-2505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1_1147-2505_I
FEATURES             Location/Qualifiers
     source          1..9543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6828..6869
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6879..6896
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6919..6936)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6969..7103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6993..7012)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7047..7066
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7725..8313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7805..7824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8484..9275,9276..9344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9107..9126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9345..9449)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9517..9535
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatta agatggtgac tgtgccacca gagcctgtgc aggacaaaac
     2761 tgcctttatt ttcaacaact tgagccagct gaacataccg cagaagtgcg atgagataaa
     2821 ggagattatg accaaggagt attggccgtg gctagcgcaa tatctggtcc tcaaacgcgc
     2881 atcaatggag ttcaacttcc acacactata ttacaacttt ttggatgccc ttaaaaacgg
     2941 cgagatcaac cgattcgtaa ccaaagagac cctacgcaac atcaaggtac tcttgcgctc
     3001 cgataaggga gttattaatt tctccgatcg aagtctgttg aagaacctcg gacactggtt
     3061 gggcatgatg accttaggtc gcaatcgccc catcctgcag ttggatctgg atcttaaatc
     3121 ccttttggct gaggcttacc acaagggcca gcaggaactg ctttttgtgg ttcctttcgt
     3181 agccaagatc cttgagtcat ccgccaagtc tcgcatcttc cgatcgccca atccatggac
     3241 aatgggtatc atgtacgtgc ttgctgagct tcaccaggag ccagacctta agctgaacct
     3301 gaagtttgaa atcgaagtac tctgcaaaac acttaatctg gagttggcca agttgcgaca
     3361 agtgatctac ctgaaggatc ccaatcgaac tcaccggatt gaagagcaga tgtcgcaacc
     3421 aaaaccgaaa caacttgagc cagtggcttc tgcgccagct ctaccgcgtg aacagcaatc
     3481 cccggcacaa cctccgccac caccacaaca gcagcaacca ccgcagcagc aggtacctcc
     3541 accgccatcc tctgcggacg tggacgccca aaatgcggcc gctatgatga tggcagcggg
     3601 cggagctaat agtactcctg gatcggtatc ttcgcccaat ctacccaccg attccagcca
     3661 ggtggcacta ccgccaccag agccgcgcta ttcatacgtg gacgtaaacg tgagcaactt
     3721 ccagcttatt ggtcagcagt tggtactgcc acccaatact ccgttcctgc acgccaatcc
     3781 cggaatcaaa cacatggtcg tcaacgccgt ggagcgcacg ataaccgact ggttgcagcc
     3841 tattgtggac cgaagcatcc gcattgcctg tgccaccacc gagcagatca tccgcaagga
     3901 cttcgccctg gatgctgacg agaaccgaat gcgcactgcc gctcatcaga tggtacgaaa
     3961 ccttgccgcc ggcatggcca tgattaccgg taaggatgag atagcgcgtg ctattagcca
     4021 gaatctgcac aaggccttgc tgtcgggtct aaatgggatg cccagcatgg ctgagatcca
     4081 agctgctgct atgcagttag ccagcgaaaa tgttgagcta gtttgcgctt tcattcagaa
     4141 aacatctgct gaaaaggcag ccgcagagat cgataggcgt ctaagcaccg atttcgagac
     4201 cagaaaaatt gctcgtgaag agggcaaccg ctttgtggac gcccagatcc tcacctatca
     4261 acaggagcgt cttccagaag cagtgcgcat caaggtgggc gcggctccag ctacacttta
     4321 tgctgtgtac tcggagttcg ctagaagcat acctggcttc cagcagatga gcgatcgcga
     4381 tattgccctg tttgtgccca agccgacgga tttgtctcag ccaaatgtgt ttgctaatga
     4441 tgatagcatg gtatacgggg aactggctag caaaatggag gccttcatga acacggcaat
     4501 cggagtgccg acactccaga tccaggctag caagatgcac atgctactca acgccctgat
     4561 agcgacgcgt cgactgcgtg accaagagtc tgcctttaat ctgctgaccc gcgcggtcga
     4621 gggtttgacc gaaggattgg tcaatatgca tgaaaacatg gagcaaatga agatgtacca
     4681 aaacatccat ctccgtatcc ttggcttgct gaacaacagt ttcggtgctc caaacacaga
     4741 gcgagcggta acaaagtgtt tcttcgatat ccgagaggag gtgcgatata acgtggaggc
     4801 agctcgcgct ttgattacgt cgcactttgt caatctgaat cagtttgacg gaatgctacg
     4861 cgactgcatg gacaacggaa acaattatgt ggccatatcg ttcggtatcg cactgctgga
     4921 gcgcctcatc atggacgatc gagtgattaa tattgtttcg gacaacgaat tcatggcaac
     4981 tgttgagctg ctaggtaggc tcactcagca tcgccatcgg tatccagaat gcattgtgaa
     5041 tgctattgat acgctctgga gcggaaactt taactcgagc agcgactaca gcccgttcaa
     5101 cggcaatgat cgttacttgt cgggagcctc ccattacatc cactccggca tgcatcacgt
     5161 aaggtcatgc gatacagatg atccgccggg cttacaagag aagacggagt ttctgcttaa
     5221 ggattgggtt gcactgtaca cgcaacagaa ccagcagtca acgcgcgatg cacgcaactt
     5281 cagtgcattt gttcagaaga tgaacacata tggtatcctg aaaacggacg acttgatcac
     5341 ccgttttttc cgccaggcca cacacatttg cacggatgtg gtgtacagaa tgttcgccga
     5401 gccgagttta ccaatcaacc aggccaaaaa caagatattc cagtggatcg atgcgttcgt
     5461 ccatctgatt gcaatgctcg tgcgacactc aggcgaagca ggtaatccaa ccaccaagat
     5521 caacctgtta aacaaggtgc tgggcattgt gctgggaacg ctgattaagg accatgagat
     5581 gcgtggcgtt agcttccagc aggttggcta ccatcgcttc ttcatgatgc tgttcatgga
     5641 gctgtgcacg gccgatgtga ttctcgagtc cctgatgcac agtattgtgt cggccttcgc
     5701 ctacacatat cacttgttga atcccagcgt ggctccggga ttctgctttg cttggttgga
     5761 gctcatttcg catcgcgtct tcctgggccg catcctggtc caaattcccg ggcaaaaggg
     5821 ctggccactt tacgctcagt tgctgcagga cctttttaaa tatcttgcgc ccttcttacg
     5881 caacactgag ctcggcaaac cagtccagct tttgtacaag gggacgctac gtgttctgct
     5941 cgttctgctt cacgacttcc cggagttctt gtgcgactat cattttgggt tctgtgacac
     6001 catcccgccc aattgcgtcc agatgcgtaa catcatcctg tcggcgtttc cgcgcaacat
     6061 gcgcttgccc gatcccttca cgcccaacct aaaagtggac atgctgtcgg acagtagtaa
     6121 tgcgcctaag gtgctcagta gctacattat gaacatccag cccgcgaact ttaagaagga
     6181 tttggactcg tacctcaagg cccgggcgcc agttactttt ctatcggaat tacgtggtca
     6241 cttgcaggtg acaagcgagc caggaactcg ttacaacatg gcgctgatga atgctctggt
     6301 catgtatgtg ggaactcagg cgattgcttt gatcagaaac aaaaactttg tgcccaacac
     6361 ctcgaacata gcacacagcg cccacatgga tatcttccag aatcttgctg ttgacttgga
     6421 tacggagggt cgctatctgt tcttgaatgc gattgcgaac cagttgcgct atcccaacag
     6481 ccacacgcac tacttcagct gtgcggtgct gcatctgttt gccgaggcca actcggaggc
     6541 aattcaggag cagatcactc gcgtacttct ggaacgattg atcgtgaacc gcccgcatcc
     6601 ttggggactg ttgatcactt ttattgagct gatcaaaaat cccatctaca agttctggga
     6661 ccatgacttt gtgcactgtg cgccagagat caccaagctc tttgaatcgg tggcccgttc
     6721 ctgtctggcc aagtcgaacg tcacccagca gttgaacatg cccgtcgtcg atggcgaggg
     6781 ccaggaggta gccaccatca actgatctag agggcccgcg gttcgaaggt aagcctatcc
     6841 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     6901 tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct
     6961 cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     7021 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     7081 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     7141 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     7201 attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     7261 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     7321 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     7381 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     7441 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     7501 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     7561 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     7621 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     7681 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7741 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7801 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7861 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     7921 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7981 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8041 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8101 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8161 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8221 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8281 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8341 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8401 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8461 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8521 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8581 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8641 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8701 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8761 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     8821 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8881 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8941 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9001 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9061 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     9121 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9181 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9241 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9301 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9361 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9421 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9481 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     9541 cgt
//
