LOCUS       sequence-289075-        7903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmDCP2-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7903)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-myc-SBP-DmDCP2-dsRNAres_J
FEATURES             Location/Qualifiers
     source          1..7903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2595..2624
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2649..2762
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             5188..5229
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5239..5256
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5279..5296)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5329..5463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5353..5372)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5407..5426
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6085..6673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6165..6184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6844..7635,7636..7704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7467..7486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7705..7809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7877..7895
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctggcaa catggagcag aaactcatct ctgaagaaga cctgaatccc ggaagcggta
     2641 gcggtaccat ggacgagaag accaccggct ggcggggcgg ccacgtggtg gagggcctgg
     2701 ccggcgagct ggagcagctg cgggccaggc tggagcacca ccctcagggc cagcgggagc
     2761 cctccggcgg ctgcaagccc ctcgctagca tggagattgc cttgttgatt aacaatgctg
     2821 tggccgtggt gaccgcttcg gctagtgctg ctgtgtcggc tagtgcctcc gtgggatcct
     2881 cctccaaaga cgacaatgtg aataacctcg ccctgctggc cagcttgacc ctacagaagg
     2941 tcctgaacat taataacaat aacaatcaac attccaaaaa taataatcat tccgataact
     3001 ccaactccaa gcagcatgaa cagcagaagc aacaacaaca gccccagcgc cgcaatctgg
     3061 atatcaagtc cgaatacgac gttttgaatc agctgttgaa gaccggcgcc gtgtttacgg
     3121 ctaccccgac cgcccccatc gctattgagc gccgcaaaca aacccaacaa caacagcgcc
     3181 cctacgccca gcaagcccac cagcaacaac aacaaaaaca gcgtctgaat tcgagcattt
     3241 ccagcacgca ttccagcacc agcaccaata gcagctatgg atcctcgtcg agcgaagacg
     3301 cagctggaac ttcggctccc actggtgctg gagaggtggt tacttccgct gctctaactc
     3361 cccgagcttc cacaacaaaa gctagctcca acaagctgcc cgaaaagagc aagatcccca
     3421 gcgacattct ggacgacttg gcttcccgct tcattattaa cgtgcccgat atggaactga
     3481 ataacctcat acgtatgtgt ttccaaatcg aactggccca ttggttctat ttggatttct
     3541 tttgtgcccc cgaatctggc gaggatggcg agaccccgaa gtgcgtgcaa cgaaagctgc
     3601 catcggtggg catcaagcag tttgccatgc agttgttcca acacattccc ttcctgaaca
     3661 aacattttgg caccgtggat caaatcctgg acgagtggaa gaactacaag ctatcggtgc
     3721 ccacttatgg cgctattctg gtgtccgagg atcacaatca ctgcttgcta gtgcagtcct
     3781 acttcgcgcg caactcctgg ggatttccca agggcaagat caatgagaac gaagacccag
     3841 cccattgtgc aacgagagag gtttatgagg agaccgggtt cgatatcacg gacctaatcg
     3901 atgccaacga ctacatcgag gcgttcatca actaccagta cacgcggctg tacgtggtgc
     3961 gaaacatacc gatggacacg cagttcgcgc cccgcactcg caacgagatc aagtgctgcg
     4021 actggttccg tatcgatgcg ctgccggtga acaagaacga tgccatatca aaggccaagt
     4081 tgggcaagac ctccaactcc ttcttcatga ttatgccgtt tgtgaagcgc ctgaagaagt
     4141 gggtgaacga tcggaaggct ggaatcgagc cgcgtcgccg caaattctct ggtcagcaat
     4201 cgccaaaagc ggcctcgccc accaatatga acggaaattg caagaaggac gtcacaggag
     4261 tgcgaaatga atcgcaacga cagaggcaca agtccatggg cgacttggat ggcgtcaaat
     4321 tgaataatct caatggcaag gggaccgttg gaacgggagc gggatcggca gcttccgcct
     4381 ccgccaccgc tgccatcaac tcgatgaaca tatgcaatag caatgggaaa cgcaataagc
     4441 aaaatgcggc tgcgggcagc aatcaggcaa cgccaactac aatagcaacg ccaatgacgt
     4501 caaatggcgc cgtcgtagga ggtactgtat cctcgaagcg tcaactgttc catagccaaa
     4561 gtcaggacga tgcgcagcaa tcggcaagca atgagaagat tgtcagctcc ttcgatttaa
     4621 tacgcaagga gaagcagcaa caacttaagg cagctaaggc gcaaaagcag cagcaacaac
     4681 agcagcaacg atcgcgcacc aagtcgcaga gtgataagca gtacaatcca cagcagccag
     4741 tcaaaatcct gggacaacag cagcccacaa cgggcatgga tttgatagcc atgctatcgg
     4801 cagcggcggc agctcaaaag acaccaaaca acgagcagca aacacaagag aagcagcagc
     4861 agcaacaacg accacgacct gcttcagtgt cgcttaacct tggagcaggc agtgccacag
     4921 gagcaacaac atcagctccg gatgcccagt acatgcacag gctgcagcgc atggcatcgg
     4981 gcacaaattt gaggattctg aagcgggcac agtcgcagca gccgcagcag caccatcagc
     5041 aacagttgca actagaacag caacaacagc agcagcagca gctgggaatc gactttacgc
     5101 caaatttgaa ttcgtggacg aatttctcct ttaccaagaa cttcatagcc aatgtgtttt
     5161 gctaatctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     5221 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     5281 tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga tccagacatg
     5341 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5401 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5461 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     5521 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gtcgaccgat
     5581 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     5641 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     5701 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     5761 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     5821 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     5881 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     5941 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     6001 tcagggacag cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg
     6061 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6121 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6181 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6241 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     6301 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6361 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6421 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6481 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6541 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6601 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7141 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7441 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     7501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7801 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     7861 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgt
//
