LOCUS       sequence-289078-        8463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmHPat-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8463)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-myc-SBP-DmHPat-dsRNAres_J
FEATURES             Location/Qualifiers
     source          1..8463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2595..2624
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2649..2762
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             5748..5789
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5799..5816
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5839..5856)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5889..6023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5913..5932)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5967..5986
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6645..7233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6725..6744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7404..8195,8196..8264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8027..8046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8265..8369)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8437..8455
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctggcaa catggagcag aaactcatct ctgaagaaga cctgaatccc ggaagcggta
     2641 gcggtaccat ggacgagaag accaccggct ggcggggcgg ccacgtggtg gagggcctgg
     2701 ccggcgagct ggagcagctg cgggccaggc tggagcacca ccctcagggc cagcgggagc
     2761 cctccggcgg ctgcaagccc ctcgctagca attctgcaga tatcatggat gactcgtttt
     2821 tcggctttga cacgaatcta ccggacgaag atggtggcga tggacgcatc gtcgaggcgg
     2881 aatacgatgc cctcaatgac gaaacctttg gatccgctat aaatggcgac tgggaggaag
     2941 cccatgagac gatggtacgc ctgggcggga atggcgaaag ggtgcggaag cgacccgcgg
     3001 aatctagtgg aaatacggat tttgccgatc agggaaatgg tgccttcctg cgacacgcta
     3061 atccgctatc atcggcgccc tcttcaagcg gctccactcc ggcccctttg aatgccccct
     3121 tccgacagtc tcgtcacata ggggactcgg acttggagct aaacatttcc tccatgaagt
     3181 tggacgacat ggacctgtcc tctttcacca ctgatagcga agccggtggg ctgagcaacc
     3241 gcattaagct ggactccggg gtctggggat cacagccgtt ccccaacaac caacatttgt
     3301 tccgcgagcc tatgcgcagc gccttcaagc cacagcagca gcaacatcag cagcaccaac
     3361 cacaacaaat gaagccaccg caggaagcga atgccaactt tgcactgaat ccacagcctt
     3421 ttccaaaaat tacaactctc gaggatatag agcgcaatat gatcattcag caggccattc
     3481 cgaaacaaca gcagcagagc cagcaacagg cggaacgcaa aatgtttgat gattttagtc
     3541 tgggtaacag gcagcaggtg cctacaccaa ccatgttgca gcaacagatg ttgcagcagc
     3601 agcatcatca gaaacaacag tcgcagcagc agccgccaca acaagcgcag cacaaggtac
     3661 cccctggctt cttgggcacg ccgcacacct ctccaccgag gccaaatttg ggtttccccg
     3721 gacccgagct actgcccacg ccatcgtcgc agcaacaact aaacggtatt ggtggcaacc
     3781 gagtgccgcc cggatttatt tacccgcaag gtctgcccgg gaatattccg caattgcccc
     3841 agcatcctct aatgcagcag cacatgccgc acaactttcc actgcccggc ggcaaccagc
     3901 gacctctacc caatccacaa gctctgccca cggccctgaa caatttcgcg atgcatccaa
     3961 gcttcaatgc gatgcgcgcc gctggaatcc atccaggggc ttttatgcaa cctccccatc
     4021 cgcaccagat gctgccaccg cgaatgccgc cgcacccgct gcaagctgcc acaagtctat
     4081 taggccagca gccaccgaat tccatgtata acatgttcaa catgcgacta gtgcaagaga
     4141 tccagcaaaa ccatccgctg cttcaacagg ctgctgttgt acgccaaatg caacagagca
     4201 gagcaggatc cgtggccagc gatcgtcaga gatcgcaggc gcaacagcag caacaaggac
     4261 gacgtccaga tggtacagga aattacccgc tggaagagta cgatgaatat gccaacctaa
     4321 tgagcacgcg cgacaagcac tggctaatcg gaatccagct ttcgcagctg aatacggaca
     4381 cgccgtatat cgacgactat tactacacgg tgttcaggga gcgcaaggcc caacagaacg
     4441 ggcaaatgcg caacagtcag gcgcacaagg acaaccagtt gaatcatccg cttactcagc
     4501 cacgcggcca tgcgcagctt attctcgtac agttgggcaa caagaatggt actcgcaatg
     4561 gacacggacg tgagcgacgc aactcggaga acaccaacag cacgtcaggc aacacgaaca
     4621 atggatcagg tggtaacaac aacaacctga caggctatat attctcacca ctgaagttcg
     4681 agaactcact gggaaagcta cagtacggaa gtgtgacagc tccgcgaaaa attatcgatg
     4741 ctgacattat gagtggtgaa tcaaatgccg ctgctgaagc caacgcaact tcttcatcaa
     4801 aaagctcgac tccgctgccc acctccgcga gccatgatgt aaatcccagt agcatgcgga
     4861 agtcgcgaca cattttgctt ctgattgaaa cactgtatcg ctatctgctg aagttggagg
     4921 atttggaaag cccagaagtg atggccacca ttgagctgaa aaagaaaaag gaagccgaac
     4981 ggatcgcagc ccttgagcag ctggaaatgg ccaataaaac gcccgaagaa cgggcagcag
     5041 aggcggctaa cccacagact atgaacccac agctcaaaaa taaatttaat tacgaagttg
     5101 aaactaaggc agcacttgta aacaagctaa aagcgggact tgcttttgat aaagtgattg
     5161 ctatgatgaa cgtcagaaaa ggtaaaattc tgatccgccg tattatgccg ttcatagccg
     5221 accagtcgat acgatgggtg gtctggtccg gagttttttg cagtctccag actgttgtca
     5281 agaaagataa agatgatgtc gagggagttc tttatgcact gtatccggaa ttcaagaagc
     5341 atgtgaataa ggcaacattt gagttactcg tctcgatatc ttcagcaatc actcttaacg
     5401 ataaaaagct ggcaggcatc ttctgttccc gattcggcat tctgagcctt gtggctctta
     5461 ttctgagggc tgaagagatt tatgttagcc gcaccgatgc cacgctgtcg gaagaaaatc
     5521 gccagaaatg gagggagttc cttgcccaag tggccagctg tttaaaccgg accatacaga
     5581 atcaaactat tagcgccgct atcgaaagcg acgccatcca acccttgatg gatcatttcg
     5641 agcgcttcaa ggacttgaag cttgacgcct tactagccct aattacggag gctcgccacc
     5701 agatcgacta gggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     5761 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     5821 tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct
     5881 cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     5941 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     6001 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     6061 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     6121 attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     6181 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     6241 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     6301 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     6361 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     6421 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     6481 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     6541 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     6601 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6661 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6721 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6781 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     6841 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6901 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6961 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7021 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7081 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7141 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7201 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7261 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7321 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7381 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7441 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7501 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7561 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7621 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7681 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     7741 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7801 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7861 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7921 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7981 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     8041 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8101 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8161 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8221 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8281 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8341 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     8401 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     8461 cgt
//
