LOCUS       sequence-28908-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Lomenick B, Hao R, Jonai N, Chin RM, Aghajan M, Warburton S, Wang J,
            Wu RP, Gomez F, Loo JA, Wohlschlegel JA, Vondriska TM, Pelletier J, 
            Herschman HR, Clardy J, Clarke CF, Huang J
  TITLE     Target identification using drug affinity responsive target 
            stability (DARTS).
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21984-9. doi: 
            10.1073/pnas.0910040106. Epub 2009 Dec 7.
  PUBMED    19995983
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0910040106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-12-22- 22"; volume: "106"; issue: "51"; pages: "21984-9"
COMMENT     Sequence Label: pcDNA3.1 FLAG mTOR 1968-2549 S2035T
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..76
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             236..514
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEATRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
ORIGIN
        1 ctctagactc gagcggccgc cactgtgctg gatatctgca gaattcgaca tggactacaa
       61 agacgatgac gacaagcacc cccaggccct catctaccca ctgacagtgg cttctaagtc
      121 taccacgaca gcccggcaca atgcagccaa caagattctg aagaacatgt gtgagcacag
      181 caacaccctg gtccagcagg ccatgatggt gagcgaggag ctgatccgag tggccatcct
      241 ctggcatgag atgtggcatg aaggcctgga agaggcaact cgtttgtact ttggggaaag
      301 gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat gctatgatgg aacggggccc
      361 ccagactctg aaggaaacat cctttaatca ggcctatggt cgagatttaa tggaggccca
      421 agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag gacctcaccc aagcctggga
      481 cctctattat catgtgttcc gacgaatctc aaagcagctg cctcagctca catccttaga
      541 gctgcaatat gtttccccaa aacttctgat gtgccgggac cttgaattgg ctgtgccagg
      601 aacatatgac cccaaccagc caatcattcg cattcagtcc atagcaccgt ctttgcaagt
      661 catcancatc caa
//
