LOCUS       sequence-289142-       11265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Braun JE, Huntzinger E, Fauser M, Izaurralde E
  TITLE     GW182 proteins directly recruit cytoplasmic deadenylase complexes to
            miRNA targets.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):120-33.
  PUBMED    21981923
REFERENCE   2  (bases 1 to 11265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-10-7"; volume: "44(1)"; pages: "120-33"
COMMENT     Sequence Label: pLNHA-C1-HsNot1iso2_J
FEATURES             Location/Qualifiers
     source          1..11265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             665..730
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             755..781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8053..8174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8090..8109)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8144..8163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8181..8636)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8318..8339)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8530..8549
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8663..8767
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8769..9126
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8769..8960
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8792..8812)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8977..9112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9161..9955
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9161..9955
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9215..9234)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9825..9844
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10143..10162)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10187..10234
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10563..11151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11052..11071
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      721 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      781 tcctccatct cgagctatga atcttgactc gctctcgctg gccttgtctc aaatcagcta
      841 cctggtggac aatttaacca agaaaaatta ccgagccagc cagcaggaaa tacagcatat
      901 tgtgaatcgg cacggtcctg aggcagacag gcatttatta cgctgcctat tttcgcatgt
      961 ggatttcagt ggcgatggta aaagcagtgg caaagatttc catcagactc agtttctgat
     1021 tcaggagtgt gcgttgctga ttacaaagcc aaattttatc tcgacgctgt cctatgccat
     1081 tgataatcca ttgcactatc agaagagttt aaagcctgca ccccacttat ttgcccagct
     1141 gagtaaagtg ctcaaattaa gcaaagtaca agaggtaatt tttggccttg ccctgttgaa
     1201 ttcttccagc tcagatctta gaggtttcgc tgcccagttt atcaaacaga agcttccaga
     1261 tcttctgcgt tcttacattg acgcagacgt cagtggaaat caagaaggtg gcttccaaga
     1321 tatagcaata gaggtcctac acctcctcct ctcccatctc ctctttgggc agaagggagc
     1381 ctttggagtt ggacaagaac agatagacgc ttttcttaag acgctgcgca gagattttcc
     1441 ccaagaacgc tgtcccgtgg tgctcgcacc acttttatac cctgaaaaac gggacattct
     1501 aatggacagg atcctgcctg attccggagg ggtagctaaa accatgatgg agagctcttt
     1561 ggctgatttc atgcaagaag taggctatgg cttttgtgca agtattgaag aatgtcgcaa
     1621 tataatcgtg cagtttggtg ttcgggaggt cacagctgcc caggttgcaa gggttttggg
     1681 aatgatggct cgaactcatt caggattaac agatggcatt ccattacaga gtatttctgc
     1741 tccgggcagt gggatctgga gtgatgggaa agataaaagt gatggagcac aggcacacac
     1801 atggaatgta gaagtcttga ttgacgttct taaagaactg aatccaagtt tgaatttcaa
     1861 ggaagtaact tatgaactgg accatcctgg atttcaaatt cgtgacagta aaggacttca
     1921 taatgtggtt tatggcattc agaggggttt gggtatggaa gtgttcccag tagacctcat
     1981 atatagacct tggaaacatg ctgaaggcca gctctccttc attcaacatt cccttataaa
     2041 tccagagatc ttctgttttg ctgactatcc ctgtcatact gttgccactg atattctgaa
     2101 agcaccacca gaggatgaca atcgagaaat tgccacatgg aagagcttgg atttgattga
     2161 atctctgctg aggcttgcag aggttgggca gtatgagcaa gtcaaacagc tcttcagctt
     2221 ccctatcaaa cactgtccag acatgctggt attggcctta ctacaaatta acacctcttg
     2281 gcataccttg cgccatgaac ttatctccac tctgatgcca attttccttg gaaaccatcc
     2341 taactcagct attattttgc actatgcatg gcatgggcag ggacagtctc cctcaattcg
     2401 ccaacttatc atgcatgcaa tggcagaatg gtacatgaga ggggagcagt atgatcaggc
     2461 caaattgtct cgaatacttg atgtggccca ggacttgaag gccttgtcaa tgctgctaaa
     2521 tggtactcca tttgcctttg ttattgacct tgctgcactt gcttcacgtc gtgaatacct
     2581 caaacttgat aagtggctca cagataaaat tcgagagcat ggggagcctt ttatccaggc
     2641 gtgtatgact tttttaaaga gacggtgtcc ttctattttg ggcggacttg ccccagaaaa
     2701 agaccagccc aaaagtgctc aacttcctcc agaaactttg gcgacaatgt tggcctgtct
     2761 gcaagcttgt gcagggagtg tttctcagga gctatcagaa actatcctca ccatggtagc
     2821 caattgcagt aatgttatga ataaggccag acaaccacca cctggagtta tgccaaaagg
     2881 acgtcctcct agtgctagca gcttagatgc catttctcct gttcagattg accctcttgc
     2941 tggaatgaca tctcttagta taggtggttc agctgcccct cacacccaga gtatgcaggg
     3001 ttttcctcca aatttgggtt ctgcattcag tacccctcag tcaccagcaa aagcatttcc
     3061 acccctttca acccccaatc agaccactgc attcagtggt attggaggac tttcatcaca
     3121 gcttccagta ggtggtcttg gcacaggcag cctgactggt ataggaactg gtgctcttgg
     3181 actccctgca gtgaataacg acccttttgt acagaggaaa ctgggcacct ctggactgaa
     3241 tcagcctaca ttccagcaga cggacttgtc tcaggtgtgg ccagaggcaa accagcactt
     3301 tagtaaagag atagatgatg aagcaaacag ctatttccag cgaatatata atcatccacc
     3361 acatccaacc atgtctgttg atgaggtatt agaaatgctg cagagattta aagactctac
     3421 tataaagagg gaacgagaag tatttaactg tatgctaagg aacttgtttg aagaatatcg
     3481 tttttttccc cagtatcctg ataaagagtt acatataaca gcctgcctat ttggtggtat
     3541 aattgagaaa ggactggtca cttacatggc actaggtctg gctctacgat atgttcttga
     3601 agccttacgc aagccttttg gatccaaaat gtattatttc gggattgctg cactagatag
     3661 atttaaaaac agattgaagg actatcccca gtattgtcag catttggctt ctatcagtca
     3721 ctttatgcaa tttccacatc atttacagga gtatattgag tatggacagc agtctagaga
     3781 tcctcctgtg aaaatgcaag gctctatcac aacccctgga agtattgcac tggctcaggc
     3841 ccaggctcag gcccaggttc cagcaaaagc tcctcttgct ggtcaagtta gcactatggt
     3901 aaccacctca acaactacca ctgttgctaa aacggttacg gtcaccaggc caactggagt
     3961 cagctttaag aaagatgtgc caccttctat taatactaca aatatagata cgttgcttgt
     4021 ggccacagat caaactgaga gaattgtgga gcccccagaa aatatccagg agaaaattgc
     4081 ttttattttc aataatctct cacagtcaaa tatgacacaa aaggttgaag agctaaagga
     4141 aacggtgaaa gaagaattta tgccttgggt ttcacagtat ctggttatga agagagtcag
     4201 tattgagcca aactttcata gcctgtattc aaacttcctt gacacgctga agaatcctga
     4261 atttaacaag atggttctga atgagaccta cagaaacatt aaagtgctcc tgacctctga
     4321 taaagctgca gccaatttct cagatcgttc tttgctgaag aacttgggac attggctagg
     4381 aatgatcaca ttagctaaaa acaaacccat cttacacact gacttggatg tgaaatcatt
     4441 gctgctagag gcttatgtta aaggacaaca agaattgctc tatgtagtgc cctttgttgc
     4501 caaagtctta gaatctagca ttcgtagtgt ggtttttagg ccaccaaacc cttggacaat
     4561 ggcaattatg aatgtattag ctgagctaca tcaggagcat gacttaaagt taaacttgaa
     4621 gtttgaaatc gaggttctct gcaagaacct tgcattagac atcaatgagc taaaacctgg
     4681 aaacctccta aaggataaag atcgcctgaa gaatttagat gagcaactct ctgctccaaa
     4741 gaaagatgtc aagcagccag aagaactccc tcccatcaca accacaacaa cttctactac
     4801 accagctacc aacaccactt gtacagccac ggttccacca cagccacagt acagctacca
     4861 cgacatcaat gtctattccc ttgcgggctt ggcaccacac attactctaa atccaacaat
     4921 tcccttgttt caggcccatc cacagttgaa gcagtgtgtg cgtcaggcaa ttgaacgggc
     4981 tgtccaggag ctggtccatc ctgtggtgga tcgatcaatt aagattgcca tgactacttg
     5041 tgagcaaata gtcaggaagg attttgccct ggattcggag gaatctcgaa tgcgaatagc
     5101 agctcatcac atgatgcgta acttgacagc tggaatggct atgattacat gcagggaacc
     5161 tttgctcatg agcatatcta ccaacttaaa aaacagtttt gcctcagccc ttcgtactgc
     5221 ttccccacaa caaagagaaa tgatggatca ggcagctgct caattagctc aggacaattg
     5281 tgagttggct tgctgtttta ttcagaagac tgcagtagaa aaagcaggcc ctgagatgga
     5341 caagagatta gcaactgaat ttgagctgag aaaacatgct aggcaagaag gacgcagata
     5401 ctgtgatcct gttgttttaa catatcaagc tgaacggatg ccagagcaaa tcaggctgaa
     5461 agttggtggt gtggacccaa agcagttggc tgtttacgaa gagtttgcac gcaatgttcc
     5521 tggcttctta cctacaaatg acttaagtca gcccacggga tttttagccc agcccatgaa
     5581 gcaagcttgg gcaacagatg atgtagctca gatttatgat aagtgtatta cagaactgga
     5641 gcaacatcta catgccatcc caccaacttt ggccatgaac cctcaagctc aggctcttcg
     5701 aagtctcttg gaggttgtag ttttatctcg aaactctcgg gatgccatag ctgctcttgg
     5761 attgctccaa aaggctgtag agggcttact agatgccaca agtggtgctg atgctgacct
     5821 tctgctgcgc tacagggaat gccacctctt ggtcctaaaa gctctgcagg atggccgggc
     5881 atatgggtct ccatggtgca acaaacagat cacaaggtgc ctaattgaat gtcgagatga
     5941 atataaatat aatgtggagg ctgtggagct gctaattcgc aatcatttgg ttaatatgca
     6001 gcagtatgat cttcacctag cgcagtcaat ggagaatggc ttaaactaca tggctgtggc
     6061 atttgctatg cagttagtaa aaatcctgct ggtggatgaa aggagtgttg ctcatgttac
     6121 tgaggcagat ctgttccaca ccattgaaac cctcatgagg attaatgctc attccagagg
     6181 caatgctcca gaaggattgc cccagctgat ggaagtagtg cgatccaact atgaagcaat
     6241 gattgatcgt gctcatggag gcccaaactt tatgatgcat tctgggatct ctcaagcctc
     6301 agagtatgat gaccctccag gcctgaggga gaaggcagag tatcttctga gggaatgggt
     6361 gaatctctac cattcagcag cagctggccg cgacagtacc aaagctttct ctgcatttgt
     6421 tggacagatg caccagcaag gaatactgaa gaccgatgat ctcataacaa ggttctttcg
     6481 tctgtgtact gaaatgtgtg ttgaaatcag ttaccgtgct caggctgagc agcagcacaa
     6541 tcctgctgcc aatcccacca tgatccgagc caagtgctat cacaacctgg atgcctttgt
     6601 tcgactcatt gcactgctcg tgaaacactc aggggaggcc accaacactg tcacaaagat
     6661 taatctgctg aacaaggtcc ttggtatagt agtgggagtt ctccttcagg atcatgatgt
     6721 tcgtcagagt gaatttcagc aacttcccta ccatcgaatt tttatcatgc ttctcttgga
     6781 actcaatgca cctgagcatg tgttggaaac cattaatttc cagacactta cagctttctg
     6841 caatacattc cacatcttga ggcctaccaa agctcctggc tttgtatatg cctggcttga
     6901 actgatttcc catcggatat ttattgcaag aatgctggca catacgccac agcagaaggg
     6961 gtggcctatg tatgcacagc tactgattga tttattcaaa tatttagcgc ctttccttag
     7021 aaatgtggaa ctcaccaaac ctatgcaaat cctctacaag ggcactttaa gagtgctgct
     7081 ggttcttttg catgatttcc cagagttcct ttgtgattac cattatgggt tctgtgatgt
     7141 gatcccacct aattgtatcc agttaagaaa tttgatcctg agtgcctttc caagaaacat
     7201 gaggctcccc gacccattca ctcctaatct aaaggtggac atgttgagtg aaattaacat
     7261 tgctccccgg attctcacca atttcactgg agtaatgcca cctcagttca aaaaggattt
     7321 ggattcctat cttaaaactc gatcaccagt cactttcctg tctgatctgc gcagcaacct
     7381 acaggtatcc aatgaacctg ggaatcgcta caacctccag ctcatcaatg cactggtgct
     7441 ctatgtcggg actcaggcca ttgcgcacat ccacaacaag ggcagcacac cttcaatgag
     7501 caccatcact cactcagcac acatggatat cttccagaat ttggctgtgg acttggacac
     7561 tgagggtcgc tatctctttt tgaatgcaat tgcaaatcag ctccggtacc caaatagcca
     7621 cactcactac ttcagttgca ccatgctgta cctttttgca gaggccaata cggaagccat
     7681 ccaagaacag atcacaagag ttctcttgga acggttgatt gtaaataggc cacatccttg
     7741 gggtcttctt attaccttca ttgagctgat taaaaaccca gcgtttaagt tctggaacca
     7801 tgaatttgta cactgtgccc cagaaatcga aaagttattc cagtcggtcg cacagtgctg
     7861 catgggacag aagcaggccc agcaagtaat ggaagggaca ggtgccagtt agccgcgggc
     7921 ccgggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     7981 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     8041 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8101 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8161 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     8221 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8281 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     8341 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     8401 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     8461 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     8521 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     8581 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     8641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8761 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     8821 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8881 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     8941 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9001 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9121 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     9181 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     9241 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     9301 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     9361 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     9421 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     9481 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     9541 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     9601 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     9661 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     9721 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     9781 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     9841 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     9901 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     9961 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
    10021 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    10081 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
    10141 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
    10201 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
    10261 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
    10321 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
    10381 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
    10441 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10501 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10561 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10621 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10681 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    10741 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10801 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10861 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10921 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10981 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    11041 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11101 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    11161 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    11221 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
