LOCUS       sequence-289152-        8495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmXRN1_574-1612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, 
            Weichenrieder O, Coles M, Izaurralde E
  TITLE     A direct interaction between DCP1 and XRN1 couples mRNA decapping to
            5' exonucleolytic degradation.
  JOURNAL   Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 
            10.1038/nsmb.2413. Epub 2012 Nov 11.
  PUBMED    23142987
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2413";
            journalName: "Nat Struct Mol Biol"; date: "2012-12"; volume: "19"; 
            issue: "12"; pages: "1324-31"
COMMENT     Sequence Label: pAc5.1A-DmXRN1_574-1612-V5His6_J
FEATURES             Location/Qualifiers
     source          1..8495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             5780..5821
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5831..5848
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5871..5888)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5921..6055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5945..5964)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5999..6018
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6677..7265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6757..6776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7436..8227,8228..8296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8059..8078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8297..8401)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8469..8487
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgcccaa
     2641 catgcgacac ggtcccatgt atgtgtacaa atacagtacg gtggcgcagg gtcccatgcc
     2701 agcgtatcca ccgctgaggg ctcttcccgt gctctactgc acggaggtgg ccaagtggtc
     2761 gcacgaaatc gcagtcaatc tgccgtacag cgtgtgcatc gagctaccaa atgcggcgag
     2821 aaccgttttc ttcccgggat ttcccaccat gcagcatctg cccttcgatt tcgagctgcg
     2881 caacgaccgc gtcaaggtgt tcgaacaggt cagtcgcaac cagaacattg tgctaaaacc
     2941 acgcaagcgc cagctggagg acacactgac ggcggtggcc tcccagtacc tcgggaaggt
     3001 gatccacgtc ggctggccgc atctcgtcaa ggcgatagtg gtgcgcgtgg ccacgcgaga
     3061 tcagcgcgtc gacagcgagg gaatcacgct aaacgattcg cggcgtttcg actcggaatg
     3121 caaggcactg caggaacact ttatcaatcg tatgggaata cagtttgcca actatgatgt
     3181 gctcgtctat gtgcgcacct ttgccggcaa ttcgacggag ttccgcgaca agggggcatt
     3241 gatggtgcgc gactcgtgga gcagctccgt caccggctat ccagcccagg gcgtggtggc
     3301 cgatctgact gtgtgggaaa gaatgcgcaa gaatttcctc aacgtggagc actacttccc
     3361 cgttggcagc acgatcttct tgatcacgga tccgtattac ggcagcgagg gaactgtgca
     3421 ggacccacgg ctggcctaca ccaacggccg gattcaagtc agcatcatgg tgcgaccgga
     3481 accgaaagta aatgcggcac gtcagctgca ggaagagcgg gaccgggact atctgagcac
     3541 cttccaggtc tgcaacttgc tccgaatcag tggccgcacc ctgggacgcc taagtggcac
     3601 cgtgtgggtg gtgctcggtc cgcggcgtca gaagatggaa aacgtgacca agcacaacat
     3661 tggcctgcag ctcaagtatc cgcggcagaa cgaggagcgt gccggatact gcttccgcac
     3721 gaacaaccag tggtactact ccagcctggc cgtggatctg atgcgtaact attgccagcg
     3781 ttacccagac gttatcgact tctttggcga ctccaacgat cgcgccgagt tcgtattcga
     3841 acaggacgtg ttccccaatg cggttggcca tcgccgcgtc gaggaactgg ccaactgggt
     3901 gcgccagcag ccgcacatga aagtggagcg catctcttgc ggctccaaga cggtctgccg
     3961 cgagaccatc gaactgctga tagccgccgt cgacgacctg cgctcactgc ccgtcaagca
     4021 cgttaaactg caggtgaagc cgcacttgct catcaagccc aatgtcacac tgccagatgt
     4081 ctaccgatca aagcgcccgg tgcgtctctt cgatcgcgtc gtaatcgtac gcaccattta
     4141 catggtgccc gtgggcacta agggcaccgt gattggcatt catcccgtta cggaccccaa
     4201 cccggtccgc ctggagtgcg tccatgctgt cgacacgttc tgcaaggtgc tgttcgacag
     4261 ccccgttccg aactgcaaca acatccatgg catcgccgaa gaccgagttt acaaggtgcc
     4321 cgaaattgcc ctggtgatca tcaagacgga tgaggaagga aagaagcaga acgactgcga
     4381 actgccggtg agggatcctc agccgaacca ggcacaggac gagccagtcc gcgcgaccag
     4441 cagtcgctat gtgaccgccg cagggtccac ctcggtcccg atcacgatga agacccaaat
     4501 tagcgacgaa ttcgtcaaga cgcgtagcga tccgatagcc cgtacggatt cgtacaagcc
     4561 atcgtcggag ccaaaaccgg tgccggtgcc ggagcagatt accaactggc gggaacgcgt
     4621 cagtacgcca acaaacaaac cgcagcctgc gccaaataat tggcgcataa atcggtcttc
     4681 atcacgtcaa cagggcggta gtatttttgt tgctccgccc acaaaaactc ctgatgcagc
     4741 tgcaagcact gcttccactg cattcaccgc agcctcgtcc gcaacgctga ctcctctgga
     4801 tcagaccttg gcgctcatgt cggttttggg cgtgggtgag gaccagagct cgccgccact
     4861 ccaggaggcg gtgcagcagc aacgtccgcc actacttcaa cagcaaagag ctccgtttcc
     4921 cggacaaatg cccaatctac ccaagccacc gctcttctgg cagcaagagg cgcagaagca
     4981 ggaggcgctg cagcaggagg cgcagcagca ggaggcgcag aagaagcagc agcaggcgca
     5041 tgcgcaaatg gagcctgaac gtatcaatag ccagcatttc tacagaagcg gccagacagg
     5101 agcggcgttg aatcagccgc cattgggtgc tccatcaaaa aggcagtggc atgagtgggt
     5161 gcacccgcgc atgcagcacg ccaacgcatt ccacgctggc gtcaacaatg gatatcagat
     5221 gcgtcccaaa aagaatatag ctgcgcagag cacctttaac aacaacgtgc acatgcacct
     5281 ccagcaacca tattacccga atcagcagca gcagcagcag cagcagcagc cactccagct
     5341 gacggagatc aacaatgcgc caccgcgata ctcaacgatc caagattttg tgcccataca
     5401 ggcgtaccgt cccaagaaat taaatcgcgt ccaaccagcc ggtcgtcagg acgtcgatgc
     5461 caccaagaat cctagtcgtt cgcctgtgtt gcagcaaccg acgaacgaga ctatagacac
     5521 aaaggccagt tctagtcttc cggtacaatc agcaggcgaa caggtgattg gactgatgca
     5581 aacgcttgaa attaagccgg ctgcgtcgca gtccgaatct gatggggtct caacaggcag
     5641 tgcgaacgcg cccacagcaa cgacatcatc ccaggcggtc aaccgaagga agcatcgagt
     5701 gcctcgcatc ggagccaagt tcgacttgga atacatactg cccgactccc cgcatccaac
     5761 ttctagaggg cccttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     5821 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     5881 gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata
     5941 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6001 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     6061 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     6121 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga
     6181 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     6241 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     6301 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     6361 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     6421 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     6481 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     6541 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     6601 agcttcaagg atcgctcgcg gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg
     6661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6841 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     6901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7081 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7561 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7741 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8041 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8341 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8401 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     8461 cctataaaaa taggcgtatc acgaggccct ttcgt
//
