LOCUS       sequence-289166-        7140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCCR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7140)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-V5-SBP-DmCCR4_K
FEATURES             Location/Qualifiers
     source          1..7140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     regulatory      2590..2599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2599..2640
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2662..2775
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(4516..4533)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4566..4700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4590..4609)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4644..4663
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5322..5910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5402..5421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6081..6872,6873..6941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6704..6723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6942..7046)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7114..7132
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctggcgg ccaccatggg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2641 cccggaagcg gtagcggtac catggacgag aagaccaccg gctggggggg cggccacgtg
     2701 gtggagggcc tggccggcga gctggagcag ctgcgggcca ggctggagca ccaccctcag
     2761 ggccagcggg agccctccgg cggctgcaag cccccaagct tgaattctat gaaaggcaat
     2821 cattataaaa tgtctcgtaa caaagacaaa tacgacagcg ccaatcggcg gcagcagatc
     2881 tttctgtcgc aggaggacat agctgcgggc aagaagacga actggagtgg cctagagatt
     2941 actggctgcg tccgcaatat cagcccgtcg ctgtgggaat ttgagcatct aaccgccctc
     3001 tacctgaacg acaaccagct gctgcgattg cccgcagacg ttggcatgct gaccagcctc
     3061 cgaactttgg acctgtccag caataagcta agaagtcttc ccgcagagct cggcgagctc
     3121 atccagttgc gggaactgtt gctgaacaac aactttctgc gcgtactgcc ttacgagatc
     3181 ggcaagctgt tccacctcgt catactcggc ctcatgggca atccgctgca aaaggagttc
     3241 atgaacatct acaacgaacc gaacggcacg cagaaactgc tcacctacat gctggacaac
     3301 ttgtcattta ccgtgaatcc accgccccag aggccctggc tgccactggc caagcccaac
     3361 aaaacgcgac cagcctgcat ttttacggtc atgtgctata atgtgctctg cgacaagtac
     3421 gcgacgcgac aaatgtacgg atactgtccg tcgtgggcgc tatgctggga gtaccgaaaa
     3481 aagtcgatta tcgacgagat acggcactat gcagcggaca ttatcagtct gcaggagatc
     3541 gaaacggagc aattctatca cttcttcctg ccggaactca agaacgatgg atacgaggga
     3601 atcttctcac cgaagtcgcg tgctaagact atgtccgagc tggagaggaa gtacgtcgat
     3661 ggctgtgcga tattcttcag ggcgtccaag tttacgctga tcaaggaatc attgatcgag
     3721 ttcaatcagc tggcaatggc caatgccgag ggctccgaca acatgctgaa ccgcgtaatg
     3781 cctaaggata acatcggtct ggccgcactg ctcaaggtga aggagaacgc atgggagccg
     3841 atgtccgagg tgacgcagat ctcgcagccg ctgctcgtct gcacggcgca catacactgg
     3901 gaccctgagt tctgcgacgt caagctcatc cagacgatga tgcttagcaa tgagttgaag
     3961 acgatcatcg acgaggcgag ccacagtttc cgacctggtc acaagaacga ctccaatgct
     4021 gtccagctgc tgctgtgcgg tgacttcaac tcgctacccg attcaggcgt tgtggagttt
     4081 ctcggcaagg gccgcgtttc catggatcat ttggacttca aggacatggg ctacaagtcc
     4141 tgcctgcagc ggctgctctc gaacgacacc aacgagttta cgcactcgtt caagctagcc
     4201 tccgcctaca acgaggacat catgccgcac accaactata cgttcgattt taagggcatc
     4261 atcgactaca ttttctacac gaagacgggc atggtgccgc tgggcctgct gggtcctgtc
     4321 tccaatgatt ggctgcgcga gaataaggtt gttggatgcc cacatccgca tataccctct
     4381 gatcacttcc cactgctggt cgagctagag ctgatgcata cggctagcca acaggcgcct
     4441 cctaacgggc tgatcaatcg ccggtagggc ggccgctcga gtctagaggg cccgcggttc
     4501 gaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga
     4561 tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4621 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4681 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     4741 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     4801 atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     4861 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     4921 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     4981 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     5041 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     5101 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     5161 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     5221 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccag
     5281 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5341 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5401 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5461 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     5521 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5581 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5641 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5701 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5761 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5821 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5881 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5941 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6001 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6061 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6121 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6181 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6241 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6301 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6361 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     6421 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6481 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6541 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6601 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6661 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     6721 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6781 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6841 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6901 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     7081 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
