LOCUS       sequence-289168-        8010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8010)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-V5-SBP-DmNot3_K
FEATURES             Location/Qualifiers
     source          1..8010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     regulatory      2590..2599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2599..2640
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2662..2775
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
     primer_bind     complement(5386..5403)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5436..5570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5460..5479)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5514..5533
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6192..6780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6272..6291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6951..7742,7743..7811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7574..7593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7812..7916)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7984..8002
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctggcgg ccaccatggg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2641 cccggaagcg gtagcggtac catggacgag aagaccaccg gctggggggg cggccacgtg
     2701 gtggagggcc tggccggcga gctggagcag ctgcgggcca ggctggagca ccaccctcag
     2761 ggccagcggg agccctccgg cggctgcaag cccccaagct tgatggctgc gacgagaaaa
     2821 ttgcaaggcg aaattgatcg atgtttaaaa aaggtagcag agggcgttga aacgtttgag
     2881 gatatttgga aaaaagtaca caatgcgaca aacaccaatc agaagcaaaa acatctgcag
     2941 gagaagtatg aggcagacct caaaaaggaa atcaagaaat tgcaacgact tcgcgaccaa
     3001 attaaatcat ggattgcatc agctgaaatt aaggataaga gctcccttct tgagaatcga
     3061 cgtctcattg gaacacaaat ggaacgcttt aaggtggttg agcgcgagac gaaaacaaaa
     3121 gcctactcca cggaaggctt gggggctgca caaaagatgg atcctgccca gcgtattaaa
     3181 gacgacgcac gcaattggtt aaccagctca ataagttccc ttcaaataca aattgaccag
     3241 tatgagagcg agatcgaatc gttgttggca ggtaaaaaga aacgtttaga tcgagacaag
     3301 caggagcgaa tggatgacct acgaggaaag ttggaccgcc ataagttcca cataacaaag
     3361 ctggaaactc tgcttaggct gcttgacaat gacggcgtcg aagcagagca ggtaaacaag
     3421 atcaaagacg atgtcgaata ctacattgat tcatcgcaag agccggactt tgaggaaaat
     3481 gagttcatat acgatgatat aattggtctt gatgaagtgg agctaagtgg cacggctacc
     3541 acggacagca acaacagcaa cgagacaagc ggttctccaa gcagtgtcac atccggaggc
     3601 agtccatcac agtcccctgt cacggttcaa caaattttaa acacttcgtc gcagggggca
     3661 gctagtagtg gtagcagcgc agctagcgca gcactgtttc agcagcagct gacagcggca
     3721 caatcgaatg gcaacaatgt gggctacgcg agcgacacaa gtgcagcttc ttcgtctgct
     3781 acaacatcta cagatcctgc aggcggcaca gttgctgtaa attgtgttgg agggctagcc
     3841 gataaacgca acaagagcag cgaaagcaat gccctaaaat tgaaaccaca acctcatcaa
     3901 cttataaaac ctacccctgt acgagccacg gccaagctgc ctctgagcag tgatacccaa
     3961 gtcaataaga ttgtcagttc aactcccagc aaaaatcagc aacagctgcc tacagcagct
     4021 tcaatcgttg caacctccgc tatgcaatcg caaagcagta tcggaagttg cagctccact
     4081 ggaggaacgg gagcatctca atcagcttct agtggtaaca atcctggtaa caatcctgcg
     4141 gttcagccaa atgctccaac gcctggtcaa agcggcattg ccgctgcagc agcttcgact
     4201 aatgttgttt cggcgacgat tgtgagcagc gccaatgttc aaggccaatc tgtgatccaa
     4261 ccaacaccaa caattgcatt tgctgcagta gcaaaacaca atacatcact cctggaaaat
     4321 ggtcctgttt tgcaacagca actagcagta actcctactg tggcagctat cgtaggagct
     4381 ggaacgcagg cgcagcaaaa acatgtgcca ccattatcca atcttcaaac aaattcccct
     4441 cacatacaaa atggtcttcc tgtctcggac agtactaatg acaacagctg caatgtcgtc
     4501 gatacgattt ctctaaaaac aatggcacag gatgctatta atcgatctgc gatcgatcct
     4561 aattcactga atcagcagca aacaagcagc atcgatctga gacagtcgca gtcgcagaaa
     4621 tcacttctcc aacatttcaa ctctgaaacc aacacgaatc aacagcaatt gacatcgcag
     4681 cagcagcaac agcttcagaa taactccctt gctgcgacca caggttctaa caacggaact
     4741 tcaactggta gtggtctcat gaatgtagca aacgctactg gtcagcctat cagcggcaat
     4801 gccaaaacgc atgcgtgcca accccaacag atggccacga cggaagcgca cattcccaca
     4861 ctgcttggtg tgacaccact tgggccaaca ccacttcaaa aggagcacca aatgcagttc
     4921 caaatgatgg aagctgccta ctatcacctg ccacagccta tggacacgga aaagctacaa
     4981 acgtactttc atcgcgcacc agtgctcacg ccatcgcact atccccaggc acagatgcca
     5041 atatatgata cagttgagtt ttatcaacga ctctcaacgg agaccctttt ttttgtgttc
     5101 tactatatgg agggctccaa ggcccagtat ctagcggcca aggccttaaa aaagcagagc
     5161 tggcgtttcc acacaaagta tatgatgtgg tttcaaaggc acgaggagcc gaagattatc
     5221 aacgatgatt acgaacaggg tacgtacatc tattttgatt atgaaaagtg gagtcagcga
     5281 aagaaggagg gattcacctt tgaatacaag tacttagagg acaaggagct gaattgaggc
     5341 ggccgctcga gtctagaggg cccgcggttc gaacccgctg atcagcctcg actgtgcctt
     5401 ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg
     5461 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     5521 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     5581 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     5641 aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc
     5701 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     5761 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     5821 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     5881 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     5941 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     6001 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     6061 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     6121 caaggatcgc tcgcggctct taccagccag caaaaggcca ggaaccgtaa aaaggccgcg
     6181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6361 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     6421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6601 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     6661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6901 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7081 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7261 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7561 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7861 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7921 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     7981 aaaaataggc gtatcacgag gccctttcgt
//
