LOCUS       sequence-289170-        5825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsDCP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5825)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCMV-TAPtagN-HsDCP2_K
FEATURES             Location/Qualifiers
     source          1..5825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      658..667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..867
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             868..1041
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1078..1098
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1105..1182
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     polyA_signal    2613..2734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2650..2669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2704..2723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2741..3196)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2878..2899)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3090..3109
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3223..3327
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3329..3686
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3329..3520
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3352..3372)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3537..3672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3721..4515
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3721..4515
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3775..3794)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4385..4404
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4703..4722)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4747..4794
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5123..5711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5612..5631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtgcc
      661 accatggcag gccttgcgca acacgatgaa gccgtggaca acaaattcaa caaagaacaa
      721 caaaacgcgt tctatgagat cttacattta cctaacttaa acgaagaaca acgaaacgcc
      781 ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa
      841 aagctaaatg atgctcaggc gccgaaagta gacaacaaat tcaacaaaga acaacaaaac
      901 gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa cgccttcatc
      961 caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc taaaaagcta
     1021 aatgatgctc aggcgccgaa agtagacgcg aattgtgata tacctacaac tgcttctgaa
     1081 aatttatatt ttcaaggtga actaaagaga agatggaaaa agaatttcat agccgtctca
     1141 gcagccaacc gctttaagaa aatctcatcc tccggggcac ttgatccatc cggactcaga
     1201 tctcgagcta tggagaccaa acgggtggag attcccggca gcgtcctgga cgatctctgc
     1261 agccgattta ttttgcatat tcccagcgag gaaagagaca atgcaatccg agtgtgtttt
     1321 cagattgaac ttgcccattg gttttacttg gatttctaca tgcagaacac accaggatta
     1381 cctcagtgtg ggataagaga ctttgctaaa gctgtcttca gtcattgtcc gtttttgctg
     1441 cctcaaggtg aagatgtgga aaaagttttg gatgaatgga aggaatataa aatgggagta
     1501 ccaacatatg gtgcaattat tcttgatgag acacttgaaa atgtactact ggttcagggg
     1561 tacctagcaa aatcaggctg gggatttcca aaaggaaaag taaataaaga agaagctcct
     1621 catgattgtg ctgctagaga ggtctttgaa gaaactggtt ttgatatcaa agactatatt
     1681 tgtaaggatg attacattga acttcgaatc aatgaccagc ttgctcgttt gtacatcatt
     1741 ccaggaattc caaaagacac aaaatttaac ccaaaaacta gaagagaaat tcggaacatt
     1801 gagtggttct ctattgagaa attgccttgt catagaaatg atatgacccc caaatccaaa
     1861 cttggtttgg cacctaacaa attttttatg gccattccct ttatcagacc attaagggac
     1921 tggctttctc gaagatttgg cgattcctca gacagtgaca atggattttc ctcaactggt
     1981 agcacgccgg ctaaacccac tgtggaaaaa ttgagtcgaa ccaaattccg ccacagtcag
     2041 cagttatttc ctgacggttc tcctggtgac cagtgggtaa agcacaggca accactgcag
     2101 caaaagccat ataataatca ttctgaaatg tctgaccttt taaaaggaaa gaatcaaagt
     2161 atgaggggaa atggcagaaa acagtatcaa gattcaccta atcaaaagaa aagaacaaat
     2221 gggcttcagc cagcaaagca gcagaattct ttgatgaagt gtgaaaagaa acttcatcca
     2281 cggaaacttc aggataattt tgaaacagat gctgtatatg acttgcctag ctccagtgaa
     2341 gaccagttgc tagaacatgc cgagggacag cccgtggcat gtaatggaca ttgcaagttc
     2401 cccttttcat ccagagcctt tttgagtttc aagtttgacc ataatgctat aatgaaaatc
     2461 ttggaccttt aaccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
     2521 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2581 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2641 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2701 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
     2761 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2821 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2881 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2941 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3001 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3061 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3121 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3181 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3301 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     3361 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3421 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3481 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3541 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3601 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3661 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     3721 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3781 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3841 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3901 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3961 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4021 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4081 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4141 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4201 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     4261 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4321 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4381 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4441 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4501 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     4561 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4621 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4681 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     4741 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     4801 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4861 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4921 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     4981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     5821 tgcat
//
