LOCUS       sequence-289189-       10620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6A-del1352-1369-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6A-del1352-1369-siRNAr...
FEATURES             Location/Qualifiers
     source          1..10620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1179..1205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5366..5377
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6339..6358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6393..6412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6631..7086
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6718..6737)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6928..6949
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7203..7560
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7203..7394
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7226..7246)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7411..7546
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7611..8405
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7665..7684)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8275..8294
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8469..8517
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8558..8577)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8677..8699
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8737..8755)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8823..8927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8928..8996,8997..9788)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9146..9165)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9959..10547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10448..10467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agatggctta cccatacgat gttccagatt
     1201 acgctatgga tgctgattct gcctccagtt ctgaatcaga gagaaacatc actatcatgg
     1261 cttcagggaa cacaggtggt gaaaaagatg gccttcggaa tagcactgga cttggttccc
     1321 aaaacaagtt tgtagttggt agcagcagca ataatgtggg ccatggaagt agtactgggc
     1381 catggggttt ttcccatgga gccataataa gcacatgtca ggtctctgtg gatgctcctg
     1441 aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta agttcttcat
     1501 caaatggagg gttaaatcca agcactttga attcagctag caaccatggt gcctggccag
     1561 tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt tctggcatta
     1621 atattcagtg cagtactata ggccagatgc ctaacaatca gagtattaac tctaaagtga
     1681 gtggtggttc tacccatggt acctggggaa gccttcagga aacttgtgaa tctgaagtaa
     1741 gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa tattaccact gaaatgactg
     1801 gaccaaataa cactactaac tttatgacct ctagtttacc aaactccggt tcagtgcaga
     1861 ataatgagct gcctagtagt aacacagggg cctggcgtgt gagcacaatg aatcatcctc
     1921 agatgcaggc tccatcaggt atgaatggca cttccctttc tcaccttagc aatggagagt
     1981 caaaaagtgg aggctcttat ggtactacat ggggtgccta tggttctaat tactctggag
     2041 acaaatgttc aggccctaat ggccaagcta atggtgacac tgtgaatgca actctaatgc
     2101 agcctggcgt aaatggtcct atgggcacta actttcaagt taacacaaac aaaggaggtg
     2161 gtgtgtggga atctggtgca gcaaactccc agagtacatc atggggaagt ggaaatggcg
     2221 caaattctgg aggaagtcga agaggatggg gaacccctgc acaaaacact ggcactaatt
     2281 tacccagcgt tgagtggaac aaactgccta gcaatcagca ttccaatgat agtgcaaatg
     2341 gcaatggtaa gacgtttaca aatggatgga aatctactga ggaagaggat cagggttctg
     2401 ccacatctca gacaaatgag caaagcagtg tgtgggccaa aacaggaggt acagtggaga
     2461 gcgatggtag tacagaaagc actggacgcc ttgaggaaaa aggaactggg gaaagtcaga
     2521 gtagagacag aagaaaaatt gatcagcaca cattactcca aagcattgta aacagaactg
     2581 acttagatcc acgtgtcctg tccaactctg gttggggaca gactcctatt aagcagaata
     2641 ctgcctggga tacagaaaca tcacctagag gggaacgaaa gactgacaat gggacagagg
     2701 cctggggaag ctctgcaaca cagactttta actcaggggc atgtatagat aagactagcc
     2761 ctaatggtaa tgatacctca tctgtatcag ggtggggcga tcccaaacct gctctgaggt
     2821 ggggagattc caaaggctca aactgccagg gggggtggga agatgattct gctgctacag
     2881 gaatggtcaa gagcaatcag tgggggaatt gcaaagagga gaaggctgca tggaatgact
     2941 cgcaaaagaa taaacaggga tggggtgatg gacaaaaatc aagccaaggg tggtctgttt
     3001 ctgccagtga taactgggga gaaacttcaa ggaataacca ttggggtgag gccaataaga
     3061 aatccagctc aggaggtagt gacagtgaca ggtccgtttc cggttggaac gaacttggta
     3121 aaactagttc tttcacttgg ggaaacaaca taaatccaaa taattcatca ggatgggatg
     3181 aatcttctaa acctactcct tcccagggat ggggagaccc tccaaagtct aatcagtctc
     3241 taggttgggg agattcgtca aagccagtca gctctccaga ctggaacaag caacaagaca
     3301 ttgttggatc ttggggaatc ccaccagcta caggcaaacc tcctggtaca ggctggctgg
     3361 ggggacctat accagcccca gcaaaagaag aagaacccac aggctgggag gaaccatccc
     3421 cagaatctat acgtcgcaaa atggagattg atgatggaac ttcagcttgg ggagatccaa
     3481 gcaaatacaa ctacaaaaat gtgaacatgt ggaacaaaaa cgtcccaaat ggcaacagcc
     3541 gttcagacca gcaagcacag gtacatcagc tgctaacgcc tgcaagtgcc atctcaaaca
     3601 aagaggcaag cagtggctct ggctggggtg agccctgggg ggagccttct actccagcca
     3661 caactgtgga taatggtact tcagcatggg gtaagcccat agacagtggt cccagctggg
     3721 gggaacccat tgctgcggca tccagcacat ccacgtgggg ctccagctct gttggtccac
     3781 aagcattaag caaatctggg ccaaaatcta tgcaagatgg ctggtgtggt gatgatatgc
     3841 cattgcctgg aaatcgcccc actggctggg aagaggaaga ggatgtggag attggaatgt
     3901 ggaatagtaa ttcatctcaa gagcttaact catctttaaa ttggccacca tatacaaaga
     3961 aaatgtcatc gaagggtctg agtggcaaaa aaaggagaag ggaaagggga atgatgaaag
     4021 gtggaaacaa acaagaagaa gcgtggataa atccatttgt taaacagttt tcaaacatca
     4081 gtttttcgag agactcacca gaggaaaatg tacaaagcaa taagatggac ctttctggag
     4141 gaatgttaca agacaaacga atggagatag ataaacatag cctaaatatt ggtgattaca
     4201 atcgaacggt cgggaaaggc cctggttctc ggcctcagat ttccaaagag tcttccatgg
     4261 agcgcaatcc ttattttgat aaggatggca ttgtagcaga tgaatcccaa aacatgcagt
     4321 ttatgtccag tcaaagcatg aagcttcccc cttcaaatag tgcactacct aaccaggccc
     4381 ttggctccat agcagggctg ggtatgcaaa acttgaattc tgttagacag aatggcaatc
     4441 ccagtatgtt tggtgttgga aacacagcag cacaaccccg gggcatgcag cagcctccag
     4501 cacaacctct tagttcatct caacccaacc tgcgggccca ggtgcctcct ccattactct
     4561 cccctcaggt tccagtttca ttgctgaagt atgcaccaaa caacggtggc ctgaatccac
     4621 tctttggccc tcaacaggta gccatgctga accagctatc ccagctaaac cagctttctc
     4681 agatctccca gttacagcga ttgttagcgc agcagcaaag ggcgcagagt cagagaagcg
     4741 tgccttctgg gaaccggccg cagcaagacc agcagggtcg acctcttagt gtgcagcagc
     4801 aaatgatgca acaatctcgt caacttgatc caaacctgtt ggtgaagcag cagactccac
     4861 catctcagca gcagccactc catcagccag ccatgaagtc tttccttgac aatgtcatgc
     4921 cccacactac acctgagctg caaaaagggc catcaccaat aaatgctttc agcaacttcc
     4981 ctataggctt gaactcaaac ttgaatgtaa atatggatat gaacagtatt aaagagccac
     5041 agtcaagact aaggaagtgg acgacagtgg acagcatttc tgtgaacaca tctttggatc
     5101 aaaactccag caaacatggt gctatttcaa gtggtttcag gctggaagag tctccatttg
     5161 ttccctatga ctttatgaac agcagtactt caccagccag tcctccaggt tcaataggag
     5221 atggctggcc acgtgccaaa tcgcctaacg gctctagcaa cattgaccct gaaactgacc
     5281 cttacgtcac tcctggcagt gtcataaaca atctttcaat taatactgtg cgggaagttg
     5341 accacctcag ggacaggaac agtgggtcat cctcatcctt gaacaccacg ctgccttcaa
     5401 ctagtgcctg gtcatccatt cgtgcctcca actacaacgt tcccctcagc agtacagcac
     5461 aaagcacttc agccagaaat agtgattcca aattgacatg gtctcctggt tcagttacaa
     5521 acacctctct ggctcatgag ctgtggaagg tccctttgcc acctaaaaac atcactgctc
     5581 cgtcccgccc acctccggga ctgactggtc agaagccacc cttgtctacg tgggataatt
     5641 ctccccttcg tataggtgga ggatggggaa attctgacgc cagatatacc ccaggttcca
     5701 gctggggtga gagcagctca gggagaataa caaattggct tgttctaaaa aaccttacac
     5761 ctcagatcga tggctcaact ctgcgcactc tgtgcatgca gcacggcccg ctgatcacat
     5821 tccacctgaa cctccctcac ggaaatgctc tggtccgcta cagttcaaaa gaagaggtag
     5881 tggaggcaca aaagtctctg cacatgtgtg tactggggaa cactactatt cttgctgagt
     5941 ttgccagtga agaggagatc agtcgtttct ttgcacaaag ccagtctctg accccttctc
     6001 ccggctggca gtctctcggg tccagccaga gccggctggg ctccctcgac tgttcccact
     6061 cattctccag ccggaccgat ctcaatcact ggaatggtgc tgggctgtcg ggaactaact
     6121 gtggagacct tcacggcact tcactctggg ggaccccgca ttattccaca agcctgtggg
     6181 gtcccccaag cagcagcgac ccccgaggaa ttagcagccc atctcccatt aacgcttttc
     6241 tttctgttga ccacctgggt gggggtggag agtccatgta agcggccgct tccctttagt
     6301 gagggttaat gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     6361 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6421 taaccattat aagctgcaat aaacaagtaa caacaattgc attcatttta tgtttcaggt
     6481 tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     6541 ccgataagga tcgatccggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6601 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     6661 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6721 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6781 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6841 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6901 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6961 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     7021 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     7081 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     7141 cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     7201 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7261 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7321 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7381 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7441 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     7501 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7561 aagcttgatt cttctgacac aacagtctcg aacttaaggc tagagccacc atgattgaac
     7621 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7681 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7741 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     7801 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7861 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7921 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7981 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     8041 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     8101 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     8161 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8221 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     8281 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     8341 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8401 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     8461 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     8521 cgatagcgat aaggatccgc gtatggtgca ctctcagtac aatctgctct gatgccgcat
     8581 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8641 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8701 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     8761 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     8821 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8881 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8941 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9001 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9061 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9121 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     9181 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     9241 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9301 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9361 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9421 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9481 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9541 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9601 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9661 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9721 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9781 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9841 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9901 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9961 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10021 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10081 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10141 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    10201 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10261 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10321 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10381 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10441 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10501 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10561 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct
//
