LOCUS       sequence-289193-       10753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-F1389A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10753)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 10753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-F1389A_K
FEATURES             Location/Qualifiers
     source          1..10753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3530..3547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6457..6578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6468..6487)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6522..6541
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6764..7219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6851..6870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7061..7082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7336..7693
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7336..7527
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7359..7379)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7544..7679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7744..8538
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7798..7817)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8408..8427
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8602..8650
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8691..8710)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8810..8832
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8870..8888)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8956..9060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9061..9129,9130..9921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9279..9298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10092..10680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10581..10600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcatt aagtgctaaa caaaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccagt tgccaaggaa tgatcttgac ccaagagttc
     2581 tgtctaatac tggttgggga cagactcctg taaagcaaaa cactgcctgg gaatttgaag
     2641 aatcccctag gtctgaaagg aaaaatgaca atgggacaga ggcctggggt tgtgcagcta
     2701 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2761 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2821 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2881 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2941 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     3001 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     3061 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3121 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3181 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3241 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3301 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3361 gcagtgaagc aactggctgg gaagaaccct ctccaccgtc cattcgccgc aaaatggaaa
     3421 ttgatgatgg tacctcagct tggggggacc caagcaacta taacaataaa actgtaaaca
     3481 tgtgggatag aaacaacccg gtcatccaga gcagtaccac gaccaatacc accaccacca
     3541 ccaccactac cacgagcaac accacacaca gggtcgagac gccgcccccg caccaggctg
     3601 gtactcagct gaatcgatca ccgttgcttg gtccaggtag gaaagtttca tcaggctggg
     3661 gagaaatgcc taatgttcac tcaaagactg aaaactcttg gggagaacca tcctcccctt
     3721 ctaccctggt ggataatggc acagcagcat gggggaagcc acccagcagt ggcagcgggt
     3781 ggggagatca ccctgccgag ccgccggtgg catttggaag agctggcgca cctgttgctg
     3841 cctcagccct gtgcaaacca gcttcaaaat ctatgcaaga aggctggggc agtggtgggg
     3901 atgaaatgaa cctcagtacc agccagtggg aggatgaaga aggggacgtg tggaataatg
     3961 ctgcttccca agaaagcacc tcctcctgca gctcctgggg gaacgccccc aaaaaaggac
     4021 ttcaaaaggg catgaagacg tctggcaagc aggatgaggc ctggatcatg agccggctga
     4081 tcaaacaact cacagacatg ggcttcccga gagagccagc tgaggaggcc ttgaagagta
     4141 acaatatgaa tcttgatcag gccatgagcg ctctgctgga aaagaaggtg gacgtggaca
     4201 agcgtgggct gggagtgacc gaccataatg gaatggccgc caagcccctc ggctgccgcc
     4261 cgccaatctc caaagagtct tccgtggacc gccccacctt tcttgacaag gatggcggcc
     4321 tcgtggaaga gcccacgcct tcaccgttct tgccttcccc aagcctgaag ctcccccttt
     4381 cacacagtgc actccccagt caggccctgg gtgggattgc ctccgggctg ggcatgcaaa
     4441 acttgaattc ttctagacag ataccgagtg gcaatctggg tatgtttggc aatagtggag
     4501 cagcacaagc caggaccatg cagcagccgc cacagccacc agtgcagcct cttaactctt
     4561 cccagcccag tctccgtgct caagtgcctc agtttctatc ccctcaggtt caagcacagc
     4621 ttttgcagtt tgcagcaaaa aacattggtc tcaaccctgc actattaacc tcgccaatta
     4681 atcctcaaca tatgacgatg ttgaaccagc tctatcagct gcagctggca taccaacgtt
     4741 tacaaatcca gcagcagatg ttacaggccc agcgtaatgt gtccggatcc atgagacaac
     4801 aggagcagca agttgcgcgc acaatcacta atctgcagca gcagatccag cagcaccagc
     4861 gccagctggc ccaggccctg ctcgtgaagc agccaccacc gccaccgccc ccgccgcacc
     4921 tgtctctgca cccctctgca ggcaaatcgg ccatggacag cttcccctcg cacccacaga
     4981 ctcccggcct acctgacctg cagaccaaag agcagcagtc ttcacccaac acctttgctc
     5041 cttaccctct cgctggactg aacccaaaca tgaatgtcaa cagcatggac atgaccggtg
     5101 gcttgtcggt gaaggaccca tcccagtccc agtcacgcct cccccagtgg acgcacccca
     5161 actccatgga taacttgccc agtgccgctt cccccctgga gcagaaccct agcaagcatg
     5221 gtgctatccc tggaggtcta agcattgggc ctccaggtaa gtcctccatt gatgactcct
     5281 atggccggta cgatttaatc cagaacagtg agtcaccagc cagtcctccc gtagctgttc
     5341 cccatagctg gtcacgtgcc aaatctgaca gtgataaaat ctcaaatggc tctagcatca
     5401 actggccccc agaagcccat ccgggagttc catggaaagg actgcagaat attgaccctg
     5461 agaatgaccc tgacgtcact cctggcagtg tccccactgg gcctaccatc aacaccacca
     5521 tccaggatgt caaccgctac ctcctcaaga gtggaggtaa actgtcagac atcaaatcga
     5581 cgtggtcctc tggccctacc tcccacacgc aagcctctct gtctcatgaa ctatggaagg
     5641 tgcccagaaa cagtactgca cccacgaggc cacctccagg gttaaccaat cccaagccct
     5701 cctccacctg gggtgccagc cccctcggct ggaccagctc ctactcctcg ggttctgcct
     5761 ggagcaccga cacctcagga agaaccagca gctggctcgt tcttcgaaac ctcactcccc
     5821 agattgatgg ttctacactg cggacattgt gtttgcaaca tgggcctctt atcacattcc
     5881 acctgaatct gactcaaggc aatgctgtgg tccggtacag ctccaaggag gaggctgcca
     5941 aggcccagaa gtctctgcac atgtgcgtcc tgggaaacac taccatcctg gccgagttcg
     6001 ctggtgaaga agaagtgaat cgcttcttag cccaaggcca ggcgctgcca cccacttcca
     6061 gctggcagtc cagcagcgcg tccagccagc cgcggctcag cgcagcgggc agctcccatg
     6121 gcctggtacg cagcgacgct ggccactgga acgccccgtg cctgggtggc aaggggagca
     6181 gtgagctgct gtggggcggg gtgccccagt actccagcag cctgtggggc ccgcccagcg
     6241 ccgacgacag cagggtgata ggcagcccca cgccgctaac caccctgctg cctggggacc
     6301 tgctcagcgg ggagtccctg taggcgctgc catcatcagc accaggagag ccgacccctc
     6361 ccgggacccc tcccggctgg gcggccccac agacccgctg gaacccagca gcggccgctt
     6421 ccctttagtg agggttaatg cttcgagcag acatgataag atacattgat gagtttggac
     6481 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6541 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     6601 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
     6661 aatgtggtaa aatccgataa ggatcgatcc gggctggcgt aatagcgaag aggcccgcac
     6721 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     6781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     6841 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6901 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6961 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7021 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7081 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7141 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7201 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7261 cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
     7321 ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     7381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7621 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7681 aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa ggctagagcc
     7741 accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7801 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     7861 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     7921 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     7981 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     8041 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     8101 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     8161 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     8221 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     8281 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     8341 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     8401 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     8461 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     8521 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     8581 acctgccatc acgatggccg caataaaata tctttatttt cattacatct gtgtgttggt
     8641 tttttgtgtg aatcgatagc gataaggatc cgcgtatggt gcactctcag tacaatctgc
     8701 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     8761 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     8821 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     8881 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8941 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9001 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9061 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9121 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9181 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9241 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9301 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9361 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9421 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9481 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9541 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9601 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9661 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9721 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9781 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9841 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9901 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9961 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10021 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10081 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10141 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10201 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10261 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10321 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10381 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10441 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10501 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10561 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10621 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10681 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10741 gctcgacaga tct
//
