LOCUS       sequence-289194-        9820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsSmg6-del42-58-del136-152-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Kashima I, Jonas S, Jayachandran U, Buchwald G, Conti E, Lupas AN, 
            Izaurralde E
  TITLE     SMG6 interacts with the exon junction complex via two conserved 
            EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay.
  JOURNAL   Genes Dev. 2010 Nov 1. 24(21):2440-50.
  PUBMED    20930030
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Nov 1. 24(21):2440-50."
COMMENT     Sequence Label: pCIneo-V5-SBP-HsSmg6-del42-58-del136-152-siRNA...
FEATURES             Location/Qualifiers
     source          1..9820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1098..1107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1107..1148
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1170..1283
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
     polyA_signal    5524..5645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5535..5554)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5589..5608
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5831..6286
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5918..5937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6128..6149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6403..6760
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6403..6594
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6426..6446)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6611..6746
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6811..7605
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6865..6884)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7475..7494
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7669..7717
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7758..7777)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7877..7899
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7937..7955)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8023..8127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8128..8196,8197..8988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8346..8365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9159..9747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9648..9667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc aattgcggcc accatgggta agcctatccc taaccctctc ctcggtctcg
     1141 attctacgcc cggaagcggt agcggtacca tggacgagaa gaccaccggc tgggggggcg
     1201 gccacgtggt ggagggcctg gccggcgagc tggagcagct gcgggccagg ctggagcacc
     1261 accctcaggg ccagcgggag ccctccggcg gctgcaagcc cctcgagaat tcacgcgtga
     1321 tggcggaagg gctggagcgt gtgcggatct ccgcgtcgga gctgcgcggg atcctggcta
     1381 ctctggcccc gcaggccggg agcagagaaa acatgaagga attaaaggag gccaggccgc
     1441 gcctaaggaa caagcccaaa atcaaggaac cccctgggag tgaggaattc aaagatgaaa
     1501 ttgttaatga ccgagattgc tctgctgttg aaaatggtac acagcccgtt aaagatgtct
     1561 gcaaggaact gaacaaccaa gagcagaatg gtcctataga cccagaaaat aatcggggac
     1621 aagaatcctt tcctaggact gctggacaag aggatcgtag tctaaaaatt atccagactg
     1681 ttagcaaaga atccgccagt cgggtggagg aggaagaagt cctcaaccag gtagaacaac
     1741 tgagagtaga ggaagatgag tgtaggggaa atgttgcgaa ggaggaagtt gcgaataaac
     1801 cagacagggc cgagatagaa aagagcccag gtggtgggag agtaggggct gcaaaaggag
     1861 aaaaaggaaa gaggatggga aaaggggagg gggtgaggga aacccacgac gacccggccc
     1921 gcgggaggcc gggctccgca aagcgctact cccgctcaga caaacgaagg aatcgctacc
     1981 gcacgcgcag caccagctca gctggcagca acaacagcgc tgagggagct ggcctgacgg
     2041 ataatggatg tcgccgccgc cgacaggata ggaccaagga gaggccacca ctgaagaagc
     2101 aagtgtctgt gtcctcaacc gattccttag acgaggacag aattgatgag cctgatggat
     2161 taggacccag gagaagttca gaaaggaaga gacatttaga aagaaactgg tctggccgtg
     2221 gggagggtga gcagaaaacc agtgctaaag aatatcgagg cactcttcgt gtcactttcg
     2281 atgcagaagc catgaacaaa gagtctccca tggtgaggtc agccagggat gatatggata
     2341 gaggaaagcc tgacaaaggc ttgagcagtg ggggcaaagg ctctgagaag caggagtcca
     2401 aaaacccgaa acaagaactt cggggtcgtg gtcgtggcat tctgattttg cctgcccata
     2461 ccaccctatc tgtcaattca gcaggttctc cagagtccgc gcctttggga cctcggcttt
     2521 tgtttggatc tggtagtaag ggatctcgga gttggggccg tggaggcacc acacgccgat
     2581 tgtgggaccc aaacaatcct gatcagaaac ctgctctaaa gactcagacg ccccagctac
     2641 atttcttgga cactgatgat gaagtcagcc ctacatcttg gggtgactca cgccaggctc
     2701 aggcatctta ctataagttt caaaactctg acaaccccta ttattacccc cggacaccag
     2761 gccctgcctc ccagtatccc tatacgggct ataaccctct acagtaccca gtgggcccta
     2821 cgaatggtgt gtacccaggg ccttactacc caggctaccc gactccgtca ggacagtatg
     2881 tgtgtagccc tctacctacc agcaccatga gtcccgagga ggtagagcag cacatgagga
     2941 acctgcagca acaggagctg cacaggcttc tccgggtggc tgacaaccag gaactgcagc
     3001 tcagcaacct gctctccagg gaccgcatca gtccggaggg cctggagaag atggcgcaac
     3061 tcagagctga actgctgcag ctatatgagc gctgtattct attagatatt gagttctctg
     3121 ataatcagaa tgtggatcag atcctgtgga agaatgcttt ctatcaggtg attgagaagt
     3181 tcaggcaact tgtcaaggat ccgaatgttg agaacccaga acagattcgg aacagacttt
     3241 tggagctctt ggatgagggt agtgacttct ttgatagttt gcttcagaag ctgcaggtta
     3301 cttacaagtt caaactggaa gactacatgg atggtcttgc cattcgcagc aagccattac
     3361 gcaagacagt aaaatatgcc ttgatcagtg cccagcgatg catgatatgc caaggagata
     3421 ttgctaggta ccgggagcag gcaagcgaca cggcaaacta tgggaaagca cgcagttggt
     3481 acctgaaggc ccagcacatt gctcccaaga atgggcgccc ctataaccag ttggctttgc
     3541 tggcagtgta tacgaggagg aagcttgacg ctgtctatta ctatatgcgc agtttagctg
     3601 ccagcaaccc tatcctgact gccaaggaga gtctcatgag cttgtttgaa gagaccaagc
     3661 ggaaggcaga acagatggaa aagaagcaac atgaggaatt tgacctgagc cctgaccagt
     3721 ggcggaaagg aaagaagtct actttccggc atgttggaga tgacaccact cgcctggaga
     3781 tctggattca tccatcccat ccacggtctt cccagggcac tgagtctggg aaggattctg
     3841 agcaagagaa tgggctgggc agcctgagtc ccagtgatct gaacaaaagg ttcatcctca
     3901 gttttctcca tgcccatggg aagctgttta cccggattgg gatggagaca ttccctgcag
     3961 tggctgagaa ggtcctcaag gagttccagg tgttactgca gcacagcccc tctcccattg
     4021 gaagtacccg catgctgcag cttatgacca tcaatatgtt tgcagtacac aactcccagc
     4081 tgaaagactg cttctcggag gagtgccgct ctgtgatcca ggaacaagcc gcagctctgg
     4141 gcttggccat gttttctcta ctggtccgcc gctgcacctg cttacttaag gagtccgcca
     4201 aagctcagct gtcctctcct gaggaccagg atgaccaaga cgacatcaag gtgtcttcct
     4261 ttgtcccgga cctgaaggag ctgctcccca gtgtcaaagt ctggtcagat tggatgctcg
     4321 gctacccgga cacctggaat cctcctccca catccctgga tctgccctcg catgttgctg
     4381 tggatgtatg gtcgacgctg gctgatttct gtaacatact gactgcagtg aatcagtctg
     4441 aggtgccact gtacaaggac ccggatgatg acctcaccct tcttatcctg gaagaggatc
     4501 ggcttctctc gggctttgtc cccttgctgg ctgcccctca ggacccctgc tacgtggaga
     4561 aaacctcgga taaggttatt gcagctgact gcaaaagggt cacagtgctg aagtattttc
     4621 tggaagccct ttgtggacaa gaagagcctc tgctggcatt caagggtgga aagtatgtgt
     4681 cagtggcacc cgtcccagac accatgggaa aggaaatggg aagccaagag ggaacacgac
     4741 tggaagatga ggaggaggat gtggtgattg aagactttga ggaagattca gaggctgaag
     4801 gcagcggagg cgaggatgac atcagggagc ttcgggccaa gaagctggct ctggccagga
     4861 agatagctga gcagcagcgt cgccaggaaa agatccaggc tgtcctggag gaccacagtc
     4921 agatgaggca gatggagctc gaaatcagac ctttgttcct cgtaccagac accaacggct
     4981 tcattgacca cctggccagt ctggcgcggc tgctggagag caggaagtac atcctggtgg
     5041 tgcccctcat cgtgatcaat gagctggacg gcctggccaa ggggcaggag acagaccacc
     5101 gggctggggg ctacgcccgt gtggtacaag agaaggcccg caagtccatc gagttcctcg
     5161 agcagcgatt cgagagtcgg gactcttgcc tgcgagccct gaccagccgt ggcaatgaac
     5221 tcgaatccat cgccttccgc agtgaggaca tcactggcca gctgggtaac aacgatgatc
     5281 tcatcctgtc ctgctgcctc cactactgca aagacaaggc taaggacttc atgcccgcca
     5341 gcaaagagga gccaatccgg ctactgcggg aggtggtgct gttgacggat gaccggaacc
     5401 tgcgtgtgaa ggcgctcaca aggaatgttc ctgtacggga catcccagcc ttcctcacgt
     5461 gggcccaggt gggctgagcg gccgcttccc tttagtgagg gttaatgctt cgagcagaca
     5521 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5581 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5641 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     5701 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat ccgataagga tcgatccggg
     5761 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5821 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5881 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5941 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6001 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6061 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6121 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6181 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6241 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt
     6301 ttctccttac gcatctgtgc ggtatttcac accgcatacg cggatctgcg cagcaccatg
     6361 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca
     6421 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6481 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6541 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6601 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6661 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6721 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttgatt cttctgacac
     6781 aacagtctcg aacttaaggc tagagccacc atgattgaac aagatggatt gcacgcaggt
     6841 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6901 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6961 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     7021 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     7081 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     7141 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     7201 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     7261 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     7321 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     7381 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     7441 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7501 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7561 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7621 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg atggccgcaa taaaatatct
     7681 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatccgc
     7741 gtatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     7801 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7861 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7921 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7981 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8041 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8101 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8161 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8221 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8281 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8341 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8401 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8461 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8521 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8581 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8641 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8701 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8761 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8821 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8881 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8941 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9001 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9061 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9301 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9781 gccttttgct ggccttttgc tcacatggct cgacagatct
//
