LOCUS       sequence-289196-        9871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsSmg6-del136-152-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9871)
  AUTHORS   Kashima I, Jonas S, Jayachandran U, Buchwald G, Conti E, Lupas AN, 
            Izaurralde E
  TITLE     SMG6 interacts with the exon junction complex via two conserved 
            EJC-binding motifs (EBMs) required for nonsense-mediated mRNA decay.
  JOURNAL   Genes Dev. 2010 Nov 1. 24(21):2440-50.
  PUBMED    20930030
REFERENCE   2  (bases 1 to 9871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 Nov 1. 24(21):2440-50."
COMMENT     Sequence Label: pCIneo-V5-SBP-HsSmg6-del136-152-siRNAres_K
FEATURES             Location/Qualifiers
     source          1..9871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1098..1107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1107..1148
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1170..1283
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
     polyA_signal    5575..5696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5586..5605)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5640..5659
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5882..6337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5969..5988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6179..6200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6454..6811
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6454..6645
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6477..6497)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6662..6797
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6862..7656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6916..6935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7526..7545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7720..7768
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7809..7828)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7928..7950
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7988..8006)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8074..8178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8179..8247,8248..9039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8397..8416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9210..9798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc aattgcggcc accatgggta agcctatccc taaccctctc ctcggtctcg
     1141 attctacgcc cggaagcggt agcggtacca tggacgagaa gaccaccggc tgggggggcg
     1201 gccacgtggt ggagggcctg gccggcgagc tggagcagct gcgggccagg ctggagcacc
     1261 accctcaggg ccagcgggag ccctccggcg gctgcaagcc cctcgagaat tcacgcgtga
     1321 tggcggaagg gctggagcgt gtgcggatct ccgcgtcgga gctgcgcggg atcctggcta
     1381 ctctggcccc gcaggccggg agcagagaaa acatgaagga attaaaggag gccaggccgc
     1441 gcaaagataa caggcgtcca gatctggaaa tctataagcc tggcctttct cggctaagga
     1501 acaagcccaa aatcaaggaa ccccctggga gtgaggaatt caaagatgaa attgttaatg
     1561 accgagattg ctctgctgtt gaaaatggta cacagcccgt taaagatgtc tgcaaggaac
     1621 tgaacaacca agagcagaat ggtcctatag acccagaaaa taatcgggga caagaatcct
     1681 ttcctaggac tgctggacaa gaggatcgta gtctaaaaat tatccagact gttagcaaag
     1741 aatccgccag tcgggtggag gaggaagaag tcctcaacca ggtagaacaa ctgagagtag
     1801 aggaagatga gtgtagggga aatgttgcga aggaggaagt tgcgaataaa ccagacaggg
     1861 ccgagataga aaagagccca ggtggtggga gagtaggggc tgcaaaagga gaaaaaggaa
     1921 agaggatggg aaaaggggag ggggtgaggg aaacccacga cgacccggcc cgcgggaggc
     1981 cgggctccgc aaagcgctac tcccgctcag acaaacgaag gaatcgctac cgcacgcgca
     2041 gcaccagctc agctggcagc aacaacagcg ctgagggagc tggcctgacg gataatggat
     2101 gtcgccgccg ccgacaggat aggaccaagg agaggccacc actgaagaag caagtgtctg
     2161 tgtcctcaac cgattcctta gacgaggaca gaattgatga gcctgatgga ttaggaccca
     2221 ggagaagttc agaaaggaag agacatttag aaagaaactg gtctggccgt ggggagggtg
     2281 agcagaaaac cagtgctaaa gaatatcgag gcactcttcg tgtcactttc gatgcagaag
     2341 ccatgaacaa agagtctccc atggtgaggt cagccaggga tgatatggat agaggaaagc
     2401 ctgacaaagg cttgagcagt gggggcaaag gctctgagaa gcaggagtcc aaaaacccga
     2461 aacaagaact tcggggtcgt ggtcgtggca ttctgatttt gcctgcccat accaccctat
     2521 ctgtcaattc agcaggttct ccagagtccg cgcctttggg acctcggctt ttgtttggat
     2581 ctggtagtaa gggatctcgg agttggggcc gtggaggcac cacacgccga ttgtgggacc
     2641 caaacaatcc tgatcagaaa cctgctctaa agactcagac gccccagcta catttcttgg
     2701 acactgatga tgaagtcagc cctacatctt ggggtgactc acgccaggct caggcatctt
     2761 actataagtt tcaaaactct gacaacccct attattaccc ccggacacca ggccctgcct
     2821 cccagtatcc ctatacgggc tataaccctc tacagtaccc agtgggccct acgaatggtg
     2881 tgtacccagg gccttactac ccaggctacc cgactccgtc aggacagtat gtgtgtagcc
     2941 ctctacctac cagcaccatg agtcccgagg aggtagagca gcacatgagg aacctgcagc
     3001 aacaggagct gcacaggctt ctccgggtgg ctgacaacca ggaactgcag ctcagcaacc
     3061 tgctctccag ggaccgcatc agtccggagg gcctggagaa gatggcgcaa ctcagagctg
     3121 aactgctgca gctatatgag cgctgtattc tattagatat tgagttctct gataatcaga
     3181 atgtggatca gatcctgtgg aagaatgctt tctatcaggt gattgagaag ttcaggcaac
     3241 ttgtcaagga tccgaatgtt gagaacccag aacagattcg gaacagactt ttggagctct
     3301 tggatgaggg tagtgacttc tttgatagtt tgcttcagaa gctgcaggtt acttacaagt
     3361 tcaaactgga agactacatg gatggtcttg ccattcgcag caagccatta cgcaagacag
     3421 taaaatatgc cttgatcagt gcccagcgat gcatgatatg ccaaggagat attgctaggt
     3481 accgggagca ggcaagcgac acggcaaact atgggaaagc acgcagttgg tacctgaagg
     3541 cccagcacat tgctcccaag aatgggcgcc cctataacca gttggctttg ctggcagtgt
     3601 atacgaggag gaagcttgac gctgtctatt actatatgcg cagtttagct gccagcaacc
     3661 ctatcctgac tgccaaggag agtctcatga gcttgtttga agagaccaag cggaaggcag
     3721 aacagatgga aaagaagcaa catgaggaat ttgacctgag ccctgaccag tggcggaaag
     3781 gaaagaagtc tactttccgg catgttggag atgacaccac tcgcctggag atctggattc
     3841 atccatccca tccacggtct tcccagggca ctgagtctgg gaaggattct gagcaagaga
     3901 atgggctggg cagcctgagt cccagtgatc tgaacaaaag gttcatcctc agttttctcc
     3961 atgcccatgg gaagctgttt acccggattg ggatggagac attccctgca gtggctgaga
     4021 aggtcctcaa ggagttccag gtgttactgc agcacagccc ctctcccatt ggaagtaccc
     4081 gcatgctgca gcttatgacc atcaatatgt ttgcagtaca caactcccag ctgaaagact
     4141 gcttctcgga ggagtgccgc tctgtgatcc aggaacaagc cgcagctctg ggcttggcca
     4201 tgttttctct actggtccgc cgctgcacct gcttacttaa ggagtccgcc aaagctcagc
     4261 tgtcctctcc tgaggaccag gatgaccaag acgacatcaa ggtgtcttcc tttgtcccgg
     4321 acctgaagga gctgctcccc agtgtcaaag tctggtcaga ttggatgctc ggctacccgg
     4381 acacctggaa tcctcctccc acatccctgg atctgccctc gcatgttgct gtggatgtat
     4441 ggtcgacgct ggctgatttc tgtaacatac tgactgcagt gaatcagtct gaggtgccac
     4501 tgtacaagga cccggatgat gacctcaccc ttcttatcct ggaagaggat cggcttctct
     4561 cgggctttgt ccccttgctg gctgcccctc aggacccctg ctacgtggag aaaacctcgg
     4621 ataaggttat tgcagctgac tgcaaaaggg tcacagtgct gaagtatttt ctggaagccc
     4681 tttgtggaca agaagagcct ctgctggcat tcaagggtgg aaagtatgtg tcagtggcac
     4741 ccgtcccaga caccatggga aaggaaatgg gaagccaaga gggaacacga ctggaagatg
     4801 aggaggagga tgtggtgatt gaagactttg aggaagattc agaggctgaa ggcagcggag
     4861 gcgaggatga catcagggag cttcgggcca agaagctggc tctggccagg aagatagctg
     4921 agcagcagcg tcgccaggaa aagatccagg ctgtcctgga ggaccacagt cagatgaggc
     4981 agatggagct cgaaatcaga cctttgttcc tcgtaccaga caccaacggc ttcattgacc
     5041 acctggccag tctggcgcgg ctgctggaga gcaggaagta catcctggtg gtgcccctca
     5101 tcgtgatcaa tgagctggac ggcctggcca aggggcagga gacagaccac cgggctgggg
     5161 gctacgcccg tgtggtacaa gagaaggccc gcaagtccat cgagttcctc gagcagcgat
     5221 tcgagagtcg ggactcttgc ctgcgagccc tgaccagccg tggcaatgaa ctcgaatcca
     5281 tcgccttccg cagtgaggac atcactggcc agctgggtaa caacgatgat ctcatcctgt
     5341 cctgctgcct ccactactgc aaagacaagg ctaaggactt catgcccgcc agcaaagagg
     5401 agccaatccg gctactgcgg gaggtggtgc tgttgacgga tgaccggaac ctgcgtgtga
     5461 aggcgctcac aaggaatgtt cctgtacggg acatcccagc cttcctcacg tgggcccagg
     5521 tgggctgagc ggccgcttcc ctttagtgag ggttaatgct tcgagcagac atgataagat
     5581 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     5641 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     5701 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
     5761 gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg gctggcgtaa
     5821 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     5881 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6061 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6181 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6301 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     6361 cgcatctgtg cggtatttca caccgcatac gcggatctgc gcagcaccat ggcctgaaat
     6421 aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa
     6481 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6541 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6601 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6661 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6721 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6781 aggctttttt ggaggcctag gcttttgcaa aaagcttgat tcttctgaca caacagtctc
     6841 gaacttaagg ctagagccac catgattgaa caagatggat tgcacgcagg ttctccggcc
     6901 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6961 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7021 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7081 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7141 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     7201 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7261 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     7321 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     7381 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     7441 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     7501 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     7561 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     7621 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     7681 ccgaccaagc gacgcccaac ctgccatcac gatggccgca ataaaatatc tttattttca
     7741 ttacatctgt gtgttggttt tttgtgtgaa tcgatagcga taaggatccg cgtatggtgc
     7801 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     7861 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7921 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     7981 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     8041 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9841 tggccttttg ctcacatggc tcgacagatc t
//
