LOCUS       sequence-289219-        5698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Braun JE, Huntzinger E, Fauser M, Izaurralde E
  TITLE     GW182 proteins directly recruit cytoplasmic deadenylase complexes to
            miRNA targets.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):120-33.
  PUBMED    21981923
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-10-7"; volume: "44(1)"; pages: "120-33"
COMMENT     Sequence Label: pLNHA-C1-HsNot11_K
FEATURES             Location/Qualifiers
     source          1..5698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             665..730
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             755..781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2486..2607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2523..2542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2577..2596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2614..3069)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2751..2772)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2963..2982
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3096..3200
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3202..3559
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3202..3393
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3225..3245)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3410..3545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3594..4388
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3594..4388
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3648..3667)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4258..4277
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4576..4595)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4620..4667
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4996..5584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5485..5504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      721 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      781 tcctccatct cgagctcaag cttcgaattc tgcagtcgac ggtacccccg gcggaggggc
      841 gagcgcggcg tctggccggc ttctcaccgc cgcggagcaa agagggtccc gggaagcggc
      901 agggtcggcg tccaggagcg gcttcggggg ctccggcggc ggcagaggcg gagcaagcgg
      961 ccccgggtcc gggagcggag gcccgggggg ccccgcgggc aggatgagct tgaccccgaa
     1021 ggagctctcg agcctgctga gcatcatatc ggaggaggcg ggcggcggca gcaccttcga
     1081 gggcctgtcc accgccttcc accactactt cagcaaggcc gaccacttcc gcctgggctc
     1141 ggtgctcgtc atgctgctcc agcagcccga cctgctgcct agcgcggcgc agcgcctcac
     1201 ggcgctctac ctgctctggg agatgtaccg caccgagccg ctggccgcca accccttcgc
     1261 cgccagcttc gcgcacctgc tcaaccccgc gccgcccgcc cgcggcggcc aggaacccga
     1321 ccgccctccg ctctcaggat ttttacctcc tataactcca ccagaaaagt tttttctttc
     1381 ccagctgatg ctggcacccc cacgggaact cttcaaaaag acgcctcgcc agattgcact
     1441 gatggacgtt ggaaacatgg gccagtctgt ggacattagt gggcttcagt tagccttggc
     1501 cgaacgccaa tctgaattgc caacgcaaag caaagcgagc ttccccagta ttctcagtga
     1561 cccagacccg gattcttcta attctggatt tgacagctca gttgcctctc agatcacaga
     1621 agctttagtc agcggaccaa agccacctat tgaaagccat tttcgaccag agtttattcg
     1681 tccaccgcct ccactccaca tttgtgagga tgaacttgct tggctaaacc ccacggagcc
     1741 tgaccacgcg atccagtggg ataaatcgat gtgtgttaag aatagcactg gtgtggagat
     1801 caaacgaata atggccaaag ccttcaaaag ccccttatcc tctccccaac aaacacagct
     1861 acttggtgag ttggaaaaag accccaaact tgtctaccat attggcctca ccccagccaa
     1921 acttcctgac cttgtggaaa acaacccttt agtcgctata gaaatgttgc tgaaattaat
     1981 gcagtcaagc cagatcactg agtatttctc tgtcctggtc aatatggaca tgtctttaca
     2041 ttcaatggaa gttgtaaatc gactaactac agctgttgat ctacctcctg aatttattca
     2101 cctttatata tcaaattgca tctctacttg tgaacagatt aaggataaat atatgcagaa
     2161 tcggttggtg cgtcttgtgt gtgtgtttct ccaatccttg atccgtaaca aaattattaa
     2221 tgtacaggat ttgtttatag aagtgcaggc attctgtatt gaattcagta ggatacgaga
     2281 agctgctggt cttttccggt tgttgaagac attggatact ggggaaacac cttctgagac
     2341 caaaatgtca aaataaggat ccaccggatc tagataactg atcataatca gccataccac
     2401 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     2461 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     2521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2581 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa
     2641 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     2701 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     2761 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     2821 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     2881 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     2941 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     3001 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     3061 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3181 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     3241 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     3301 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3361 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3421 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3481 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     3541 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     3601 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     3661 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     3721 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     3781 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     3841 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     3901 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     3961 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     4021 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     4081 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     4141 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     4201 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4261 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4321 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4381 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     4441 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     4501 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     4561 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     4621 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     4681 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     4741 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     4801 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     4861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5101 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
