LOCUS       sequence-289220-        9371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTAB182.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Braun JE, Huntzinger E, Fauser M, Izaurralde E
  TITLE     GW182 proteins directly recruit cytoplasmic deadenylase complexes to
            miRNA targets.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):120-33.
  PUBMED    21981923
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-10-7"; volume: "44(1)"; pages: "120-33"
COMMENT     Sequence Label: pLNHA-C1-HsTAB182_K
FEATURES             Location/Qualifiers
     source          1..9371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             665..730
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             755..781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6159..6280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6196..6215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6250..6269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6287..6742)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6424..6445)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6636..6655
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6769..6873
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6875..7232
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6875..7066
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6898..6918)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7083..7218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7267..8061
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7267..8061
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7321..7340)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7931..7950
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8249..8268)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8293..8340
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8669..9257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9158..9177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      721 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      781 tcctccatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccatcaaa
      841 agtgtctact ctcagggaaa gctcagccat ggcttcccca ctgccccggg agatggagga
      901 ggagctggtg cctactggct ctgagccagg tgacactcgg gccaaacccc ctgtcaagcc
      961 caaaccccgg gccctgcctg ccaagccagc cctgcctgcc aaacccagcc tgctggtgcc
     1021 tgttgggcct cggcctcccc ggggtcccct ggctgagttg ccttctgcca ggaagatgaa
     1081 catgctggca ggaccccagc cctatggtgg cagcaagcgc ccccttccct ttgcaccaag
     1141 gcctgcggtt gaggcctcca ctggaggaga agccacccaa gagactggga aagaggaggc
     1201 tgggaaagag gagccacccc ctttgacacc cccagctcga tgtgcagccc cagggggtgt
     1261 acggaaggcc cctgcccctt tccgcccagc ctcagagcgc ttcgcggcca ccacggtgga
     1321 agagatcctg gccaagatgg agcagcctcg gaaggaggtc cttgccagcc ccgaccgcct
     1381 gtggggttcc cgcctcacct ttaaccacga tggcagctcg cgatatggcc ccaggaccta
     1441 tggcacgacc actgctccca gggatgagga tggcagcacc ctcttcaggg gatggtccca
     1501 ggaggggcca gtaaagtctc cagcagagtg ccgggaagag cacagcaaga cccctgagga
     1561 gaggagcctt ccttccgacc tggccttcaa cggggacctg gctaaggcag ccagctcgga
     1621 gctacctgct gatatttcca agccctggat tccctcaagt ccagccccct cctcagagaa
     1681 tggaggccct gccagcccag gcctccccgc agaagcctca ggctcaggcc ctggctctcc
     1741 ccatcttcac ccgcctgata agagttctcc ctgccactca cagcttctgg aagcccagac
     1801 tcctgaagct tcccaggctt ctccctgccc cgctgtgact ccatcagctc caagtgcagc
     1861 cctgcctgac gagggctccc gccacacccc cagcccgggg ctccctgccg agggggctcc
     1921 agaggccccc agacccagca gcccaccccc tgaggtcttg gagccccata gcctggatca
     1981 gccccctgcc acctcacccc ggcccctgat cgaggtgggt gagttgctgg atctcactcg
     2041 gacgtttcca tctggcgggg aggaggaggc caagggtgac gcacacctcc gccccaccag
     2101 cctggttcag cgccgattct ctgaaggtgt gctccagtca cccagtcagg accaggagaa
     2161 gctggggggc tcgctggctg ccctgcccca aggccagggg agccagttgg ccctggatcg
     2221 tccctttggg gcagagtcca actggagctt atcacagtcc ttcgaatgga ccttccccac
     2281 gaggccctcg ggtctgggcg tgtggcggct ggactccccg cctccctccc ccatcactga
     2341 agccagtgag gccgccgagg ctgctgaggc tggcaacttg gccgtttcca gcagggaaga
     2401 aggagtgtct cagcaggggc aaggggctgg gtcagctcca agtgggtcag gaagttcctg
     2461 ggtgcagggg gatgatccaa gcatgtccct cacccagaag ggcgatgggg agagtcaacc
     2521 tcaattccca gctgttcccc ttgagcccct gcctacaact gagggcacac ctggattacc
     2581 tttgcagcag gcagaggaga gatacgagtc gcaggagccc ttggctggac aggagtcccc
     2641 tctccccctg gctaccaggg aggcagcctt gcccatcctg gagccagtcc tggggcagga
     2701 gcagccagca gcccctgacc agccctgtgt tctctttgct gatgcccctg agcctggaca
     2761 ggcactgcct gttgaggagg aggccgtgac cctagcccgg gctgagacca cccaagccag
     2821 gacagaggct caagacttgt gtagggcatc ccccgagcct ccaggccctg aaagcagctc
     2881 ccgctggctg gacgacctcc tggcttcacc accacccagt ggtggcggtg caaggcgggg
     2941 agctggagct gagctgaagg acacacagtc cccaagtacc tgctctgagg gactccttgg
     3001 ctggtcccag aaagatctgc agagtgaatt tgggatcaca ggagacccac agcccagcag
     3061 tttcagtcct tccagctggt gtcaaggtgc ttctcaggac tatggccttg ggggtgcaag
     3121 ccctagagga gacccaggtc tcggagagag ggactggacc agcaagtatg ggcaaggagc
     3181 aggggaaggg agcaccaggg agtgggccag caggtgtggc atcggccagg aggagatgga
     3241 ggccagcagc agccaagacc agagtaaagt gtctgcccca ggggtgctca cagcccagga
     3301 ccgggtagtt ggaaagccag cccagcttgg cactcagcgg agccaggagg cagatgttca
     3361 ggactgggag ttcagaaaga gggattccca gggcacttac tccagccggg atgcagaact
     3421 ccaggaccag gaattcggaa agagagattc actgggtacc tacagtagtc gagatgtaag
     3481 ccttggggac tgggaatttg ggaagagaga ttctctgggt gcttatgcca gccaagatgc
     3541 caacgagcag ggccaggatt tggggaagag ggaccaccat ggtaggtaca gcagccagga
     3601 tgccgatgag caggactggg agtttcagaa gagagatgtg tcactcggca cctatggcag
     3661 ccgggctgcg gagccacagg aacaggagtt tgggaagagc gcttggataa gggactacag
     3721 cagtggtggc agctccagga cccttgacgc ccaggacaga agctttggaa cgagacccct
     3781 gagctctggg ttcagccccg aggaagccca gcaacaggat gaggaatttg agaagaagat
     3841 tccaagtgtg gaagacagcc ttggagaggg cagcagggat gctggccggc caggagagag
     3901 aggatccggg ggcttgttca gtcctagcac tgcccacgtg ccggatgggg cactcgggca
     3961 gagagaccag agcagctggc aaaacagtga tgctagccag gaggtgggag ggcatcagga
     4021 gagacagcag gcaggggctc agggccctgg cagtgctgac ctggaagatg gggagatggg
     4081 aaagcgaggc tgggtcggtg agtttagcct cagtgttggc ccccagcgag aggcagcatt
     4141 tagcccaggg cagcaggact ggagccggga cttctgcatc gaggccagtg agaggagcta
     4201 tcagtttggc atcattggca acgacagagt gagtggtgct ggctttagcc cttctagcaa
     4261 gatggaaggt ggtcactttg tgcctcctgg gaagaccaca gctggctcgg tggactggac
     4321 tgaccagctg ggtctcagga acttggaagt gtccagctgt gtgggttctg ggggctctag
     4381 cgaggccagg gagagtgccg tgggacagat gggctggtca ggtggcctga gcttgagaga
     4441 catgaacctg accggctgtt tggaaagtgg agggtctgaa gagccggggg gaatcggagt
     4501 tggggagaag gactggactt ctgatgttaa tgtgaagagc aaagatttgg ctgaggtcgg
     4561 ggagggagga ggccacagcc aggccagaga gagtggcgtg gggcagactg actggtcagg
     4621 tgtggaggcc ggagagttcc ttaaatcaag ggagcgtgga gttggacagg cagactggac
     4681 acctgacctt gggctgagaa acatggcccc aggggcagtc tgcagtcctg gagagtccaa
     4741 agagcttggg gtgggccaga tggactgggg taacaatctg ggcctgaggg atttggaggt
     4801 gacctgtgac ccagactctg gaggttctca ggggctacgg ggatgtggag tggggcagat
     4861 ggactggacc caggacttgg cgccccagaa tgtggagctc tttggggctc caagtgaagc
     4921 cagggagcat ggggtgggcg gggtgagcca gtgcccagag cccggcctga ggcacaatgg
     4981 cagcttgtct cctggcctgg aggccagaga ccccttggag gccagggagc tgggggttgg
     5041 tgagacaagt gggccagaga cccagggtga agattactcc tcgtcttcct tggagccaca
     5101 ccctgcagac cctggaatgg agacaggaga agccctcagc ttcggagcaa gccctggcag
     5161 gtgcccggcc cgccccccac cctccggctc ccagggcctg ctggaggaga tgctggcagc
     5221 cagcagctcc aaggcggtgg ctcggaggga gtcagcggcc tcgggccttg ggggcctgtt
     5281 ggaggaggaa ggagccgggg caggtgctgc ccaagaggag gtgctggagc ctggcaggga
     5341 ctctccaccc tcctggaggc cgcagcctga tggtgaggcc agccagacag aagacgtgga
     5401 tggcacctgg ggctcttcag cagccaggtg gagcgatcag gggccagcac agacttctcg
     5461 gcgaccctcc caaggccctc ctgccagatc ccccagtcag gacttctcct tcattgagga
     5521 caccgagatc ctcgacagtg ccatgtatcg gagccgtgcc aacttggggc gcaagcgtgg
     5581 gcaccgggcc ccggtcattc ggcctggggg taccttgggc ctgtcggagg cagcagactc
     5641 ggatgcacac ctgttccagg actctacaga gccacgggca tctcgggtgc catcttcaga
     5701 tgaagaggta gtggaggaac ctcagagccg ccggacacgg atgtcgttgg gcaccaaggg
     5761 gctgaaagtc aacctctttc ctggcctgag cccctcagcc ctgaaggcca agctgcgccc
     5821 ccggaatcgc tcagctgagg agggagagct ggctgagagc aagtcgagcc agaaggagtc
     5881 cgcggtccag cgttcgaaat cctgcaaggt cccaggactg ggaaagcccc tcacgttacc
     5941 tcccaagcca gagaaatcct cagggtcaga aggatcgtcg cccaactggc ttcaagccct
     6001 gaaactgaag aagaagaagg tctgagatgg gatccaccgg atctagataa ctgatcataa
     6061 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6121 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6181 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6241 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt
     6301 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     6361 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     6421 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     6481 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     6541 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     6601 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     6661 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     6721 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6781 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6841 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     6901 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6961 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7021 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7081 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7141 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7201 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     7261 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     7321 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     7381 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     7441 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     7501 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     7561 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     7621 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7681 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7741 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     7801 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     7861 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     7921 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7981 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     8041 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     8101 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     8161 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     8221 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     8281 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     8341 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     8401 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     8461 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     8521 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8581 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9361 ccgccatgca t
//
