LOCUS       sequence-28924-e         534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Wordeman L, Wagenbach M, von Dassow G
  TITLE     MCAK facilitates chromosome movement by promoting kinetochore 
            microtubule turnover.
  JOURNAL   J Cell Biol. 2007 Dec 3. 179(5):869-79.
  PUBMED    18039936
REFERENCE   2  (bases 1 to 534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2007 Dec 3. 179(5):869-79."
COMMENT     Sequence Label: pMX232
FEATURES             Location/Qualifiers
     source          1..534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    127..248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(138..157)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     192..211
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(427..444)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
       61 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      121 acgccttaag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      181 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      241 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      301 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
      361 cgcggccatc ccgggcccgc ggtaccgtcg actgcagaat tcgaagctta tcgatgcggc
      421 cgcttagtga tgatggtgat ggtgttggtg ccgtttcttg ctgttcatct gtttgctagc
      481 ctgttcttcc agttgcatgg ccacacgcag gccttgatga cttctcgcag gctg
//
