LOCUS       sequence-28926-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Massey-Gendel E, Zhao A, Boulting G, Kim HY, Balamotis MA, Seligman 
            LM, Nakamoto RK, Bowie JU
  TITLE     Genetic selection system for improving recombinant membrane protein 
            expression in E. coli.
  JOURNAL   Protein Sci. 2009 Feb . 18(2):372-83.
  PUBMED    19165721
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2009 Feb . 18(2):372-83."
COMMENT     Sequence Label: pSEL1
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(313..327,328..336))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(340..372)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(376..393)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(439..604)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(497..516)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ctgagaacta tattaattct gtcctttaaa ggtcgattct tctcaggaat ggagaaccag
       61 gttttcctac ccataatcac cagattctgt ttaccttcca ctgaagaggt tgtggtcatt
      121 ctttggaagt acttgaactc gttcctgagc ggaggccagg gtaggtctcc gttcttgcca
      181 atccccatat tttgggacac ggcgacgatg cagttcaatg gtcgaaccat gccagaggtg
      241 ccagaggtgc cagaggtgcc agaggtcggc cgggtaccag ctgcagatct cgagctcgga
      301 tccccatcga tccttatcgt catcgtcgta cagatcccga cccatttgct gtccaccagt
      361 catgctagcc ataccatgat gatgatgatg atgagaaccc cccatggtta attcctcctg
      421 ttagcccaaa aaacgggtat ggagaaacag tagagagttg cgataaaaag cgtcaggtag
      481 gatccgctaa tcttatggat aaaaatgcta tggcatagca aagtgtgacg ccgtgcaaat
      541 aatcaatgtg gacttttctg ccgtgattat agacactttt gttacgcgtt tttgtcatgg
      601 ctttggtccc gctttgttac agaatgcttt taataagcgg ggttaccggt ttggttagcg
      661 agaagagcca gtaaaagacg cagtgacggc aatgtctgat gcaatatgga caat
//
