LOCUS       sequence-28927-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Massey-Gendel E, Zhao A, Boulting G, Kim HY, Balamotis MA, Seligman 
            LM, Nakamoto RK, Bowie JU
  TITLE     Genetic selection system for improving recombinant membrane protein 
            expression in E. coli.
  JOURNAL   Protein Sci. 2009 Feb . 18(2):372-83.
  PUBMED    19165721
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2009 Feb . 18(2):372-83."
COMMENT     Sequence Label: pSEL1
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..605)
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
ORIGIN
        1 cgcaacagcc agctcaaacg tcgtgagaca gtttgtagct tttagtcttt cttctcgtag
       61 acttcaaact tatacttgat gcctttttcc tcctggacct cagagaggac gcctgggtat
      121 tctgggagaa gtttatattt ccccaaatca atttctggga aaaacgtgtc actttcaaat
      181 tcctgcatga tccttgtcac aaagagtctg aggtggcctg gttgattcat ggcttcctgg
      241 taaacagaac tgcctccgac tatccaaacc atgtctactt tacttgccaa ttccggttgt
      301 tcaataagtc ttaaggcatc atccaaactt ttggcaagaa aatgagctcc tcgtggtggt
      361 tctttgagtt ctctactgag aactatatta attctgtcct ttaaaggtcg attcttctca
      421 ggaatggaga accaggtttt cctacccata atcaccagat tctgtttacc ttccactgaa
      481 gaggttgtgg tcattctttg gaagtacttg aactcgttcc tgagcggagg ccagggtagg
      541 tctccgttct tgccaatccc catattttgg gacacggcga cgatgcagtt caatggtcga
      601 accatgccag aggtgccaga ggtgccagag gtgccagagg tcggccgggt accagctgca
      661 gatctcgagc tcggat
//
