LOCUS       sequence-28928-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Massey-Gendel E, Zhao A, Boulting G, Kim HY, Balamotis MA, Seligman 
            LM, Nakamoto RK, Bowie JU
  TITLE     Genetic selection system for improving recombinant membrane protein 
            expression in E. coli.
  JOURNAL   Protein Sci. 2009 Feb . 18(2):372-83.
  PUBMED    19165721
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2009 Feb . 18(2):372-83."
COMMENT     Sequence Label: pSEL2
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(135..149,150..158))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(162..194)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(198..215)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(261..545)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(319..338)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 ttggatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc atgccagagg
       61 tgccagaggt gccagaggtg ccagaggtcg gccgggtacc agctgcagat ctcgagctcg
      121 gatccccatc gatccttatc gtcatcgtcg tacagatccc gacccatttg ctgtccacca
      181 gtcatgctag ccataccatg atgatgatga tgatgagaac cccccatggt taattcctcc
      241 tgttagccca aaaaacgggt atggagaaac agtagagagt tgcgataaaa agcgtcaggt
      301 aggatccgct aatcttatgg ataaaaatgc tatggcatag caaagtgtga cgccgtgcaa
      361 ataatcaatg tggacttttc tgccgtgatt atagacactt ttgttacgcg tttttgtcat
      421 ggctttggtc ccgctttgtt acagaatgct tttaataagc ggggttaccg gtttggttag
      481 cgagaagagc cagtaaaaga cgcagtgacg gcaatgtctg atgcaatatg gacaattggt
      541 ttcttctctg aatggcggga gtatgaaaag tatggctgaa gcgcaaaatg atcccctgct
      601 gccgggatac tcgtttaatg cccatctggt ggcgggttta acgccgattg aggccaacgg
      661 ttatctcgat ttttttatcg accgaccgct gggaatgaaa gg
//
