LOCUS       sequence-289293-       11187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6A-F1359A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 11187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pAc5.1B-EGFP-HsTNRC6A-F1359A_L
FEATURES             Location/Qualifiers
     source          1..11187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    7528..7539
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             8472..8513
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8523..8540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8563..8580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8613..8747
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8637..8656)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8691..8710
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9369..9957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9449..9468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10128..10919,10920..10988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10751..10770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10989..11093)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11161..11179
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgatggatg ctgattctgc ctccagttct gaatcagaga gaaacatcac
     3361 tatcatggct tcagggaaca caggtggtga aaaagatggc cttcggaata gcactggact
     3421 tggttcccaa aacaagtttg tagttggtag cagcagcaat aatgtgggcc atggaagtag
     3481 tactgggcca tggggttttt cccatggagc cataataagc acatgtcagg tctctgtgga
     3541 tgctcctgaa agcaaatctg aaagtagcaa caatagaatg aatgcttggg gcactgtaag
     3601 ttcttcatca aatggagggt taaatccaag cactttgaat tcagctagca accatggtgc
     3661 ctggccagta ttagagaaca atggacttgc cctaaaaggg cctgtaggga gtggtagttc
     3721 tggcattaat attcagtgca gtactatagg ccagatgcct aacaatcaga gtattaactc
     3781 taaagtgagt ggtggttcta cccatggtac ctggggaagc cttcaggaaa cttgtgaatc
     3841 tgaagtaagt ggtacacaga aggtttcatt cagtggtcaa cctcaaaata ttaccactga
     3901 aatgactgga ccaaataaca ctactaactt tatgacctct agtttaccaa actccggttc
     3961 agtgcagaat aatgagctgc ctagtagtaa cacaggggcc tggcgtgtga gcacaatgaa
     4021 tcatcctcag atgcaggctc catcaggtat gaatggcact tccctttctc accttagcaa
     4081 tggagagtca aaaagtggag gctcttatgg tactacatgg ggtgcctatg gttctaatta
     4141 ctctggagac aaatgttcag gccctaatgg ccaagctaat ggtgacactg tgaatgcaac
     4201 tctaatgcag cctggcgtaa atggtcctat gggcactaac tttcaagtta acacaaacaa
     4261 aggaggtggt gtgtgggaat ctggtgcagc aaactcccag agtacatcat ggggaagtgg
     4321 aaatggcgca aattctggag gaagtcgaag aggatgggga acccctgcac aaaacactgg
     4381 cactaattta cccagcgttg agtggaacaa actgcctagc aatcagcatt ccaatgatag
     4441 tgcaaatggc aatggtaaga cgtttacaaa tggatggaaa tctactgagg aagaggatca
     4501 gggttctgcc acatctcaga caaatgagca aagcagtgtg tgggccaaaa caggaggtac
     4561 agtggagagc gatggtagta cagaaagcac tggacgcctt gaggaaaaag gaactgggga
     4621 aagtcagagt agagacagaa gaaaaattga tcagcacaca ttactccaaa gcattgtaaa
     4681 cagaactgac ttagatccac gtgtcctgtc caactctggt tggggacaga ctcctattaa
     4741 gcagaatact gcctgggata cagaaacatc acctagaggg gaacgaaaga ctgacaatgg
     4801 gacagaggcc tggggaagct ctgcaacaca gacttttaac tcaggggcat gtatagataa
     4861 gactagccct aatggtaatg atacctcatc tgtatcaggg tggggcgatc ccaaacctgc
     4921 tctgaggtgg ggagattcca aaggctcaaa ctgccagggg gggtgggaag atgattctgc
     4981 tgctacagga atggtcaaga gcaatcagtg ggggaattgc aaagaggaga aggctgcatg
     5041 gaatgactcg caaaagaata aacagggatg gggtgatgga caaaaatcaa gccaagggtg
     5101 gtctgtttct gccagtgata actggggaga aacttcaagg aataaccatt ggggtgaggc
     5161 caataagaaa tccagctcag gaggtagtga cagtgacagg tccgtttccg gttggaacga
     5221 acttggtaaa actagttctt tcacttgggg aaacaacata aatccaaata attcatcagg
     5281 atgggatgaa tcttctaaac ctactccttc ccagggatgg ggagaccctc caaagtctaa
     5341 tcagtctcta ggttggggag attcgtcaaa gccagtcagc tctccagact ggaacaagca
     5401 acaagacatt gttggatctt ggggaatccc accagctaca ggcaaacctc ctggtacagg
     5461 ctggctgggg ggacctatac cagccccagc aaaagaagaa gaacccacag gctgggagga
     5521 accatcccca gaatctatac gtcgcaaaat ggagattgat gatggaactt cagcttgggg
     5581 agatccaagc aaatacaact acaaaaatgt gaacatgtgg aacaaaaacg tcccaaatgg
     5641 caacagccgt tcagaccagc aagcacaggt acatcagctg ctaacgcctg caagtgccat
     5701 ctcaaacaaa gaggcaagca gtggctctgg ctggggtgag ccctgggggg agccttctac
     5761 tccagccaca actgtggata atggtacttc agcatggggt aagcccatag acagtggtcc
     5821 cagctggggg gaacccattg ctgcggcatc cagcacatcc acgtggggct ccagctctgt
     5881 tggtccacaa gcattaagca aatctgggcc aaaatctatg caagatggct ggtgtggtga
     5941 tgatatgcca ttgcctggaa atcgccccac tggctgggaa gaggaagagg atgtggagat
     6001 tggaatgtgg aatagtaatt catctcaaga gcttaactca tctttaaatt ggccaccata
     6061 tacaaagaaa atgtcatcga agggtctgag tggcaaaaaa aggagaaggg aaaggggaat
     6121 gatgaaaggt ggaaacaaac aagaagaagc gtggataaat ccatttgtta aacagttttc
     6181 aaacatcagt ttttcgagag actcaccaga ggaaaatgta caaagcaata agatggacct
     6241 ttctggagga atgttacaag acaaacgaat ggagatagat aaacatagcc taaatattgg
     6301 tgattacaat cgaacggtcg ggaaaggccc tggttctcgg cctcagattt ccaaagagtc
     6361 ttccatggag cgcaatcctt attttgataa ggatggcatt gtagcagatg aatcccaaaa
     6421 catgcagttt atgtccagtc aaagcatgaa gcttccccct tcaaatagtg cactacctaa
     6481 ccaggccctt ggctccatag cagggctggg tatgcaaaac ttgaattctg ttagacagaa
     6541 tggcaatccc agtatgtttg gtgttggaaa cacagcagca caaccccggg gcatgcagca
     6601 gcctccagca caacctctta gttcatctca gcctaatctc cgtgctcaag tgcctcctcc
     6661 attactctcc cctcaggttc cagtttcatt gctgaagtat gcaccaaaca acggtggcct
     6721 gaatccactc tttggccctc aacaggtagc catgctgaac cagctatccc agctaaacca
     6781 gctttctcag atctcccagt tacagcgatt gttagcgcag cagcaaaggg cgcagagtca
     6841 gagaagcgtg ccttctggga accggccgca gcaagaccag cagggtcgac ctcttagtgt
     6901 gcagcagcaa atgatgcaac aatctcgtca acttgatcca aacctgttgg tgaagcagca
     6961 gactccacca tctcagcagc agccactcca tcagccagcc atgaagtctt tccttgacaa
     7021 tgtcatgccc cacactacac ctgagctgca aaaagggcca tcaccaataa atgctttcag
     7081 caacttccct ataggcttga actcaaactt gaatgtaaat atggatatga acagtattaa
     7141 agagccacag tcaagactaa ggaagtggac gacagtggac agcatttctg tgaacacatc
     7201 tttggatcaa aactccagca aacatggtgc tatttcaagt ggtttcaggc tggaagagtc
     7261 tccatttgtt ccctatgact ttatgaacag cagtacttca ccagccagtc ctccaggttc
     7321 aataggagat ggctggccac gtgccaaatc gcctaacggc tctagcagtg ttaattggcc
     7381 accagaagct cgtcctggtg agccatggaa aggttatcca aacattgacc ctgaaactga
     7441 cccttacgtc actcctggca gtgtcataaa caatctttca attaatactg tgcgggaagt
     7501 tgaccacctc agggacagga acagtgggtc atcctcatcc ttgaacacca cgctgccttc
     7561 aactagtgcc tggtcatcca ttcgtgcctc caactacaac gttcccctca gcagtacagc
     7621 acaaagcact tcagccagaa atagtgattc caaattgaca tggtctcctg gttcagttac
     7681 aaacacctct ctggctcatg agctgtggaa ggtccctttg ccacctaaaa acatcactgc
     7741 tccgtcccgc ccacctccgg gactgactgg tcagaagcca cccttgtcta cgtgggataa
     7801 ttctcccctt cgtataggtg gaggatgggg aaattctgac gccagatata ccccaggttc
     7861 cagctggggt gagagcagct cagggagaat aacaaattgg cttgttctaa aaaaccttac
     7921 acctcagatc gatggctcaa ctctgcgcac tctgtgcatg cagcacggcc cgctgatcac
     7981 attccacctg aacctccctc acggaaatgc tctggtccgc tacagttcaa aagaagaggt
     8041 agtggaggca caaaagtctc tgcacatgtg tgtactgggg aacactacta ttcttgctga
     8101 gtttgccagt gaagaggaga tcagtcgttt ctttgcacaa agccagtctc tgaccccttc
     8161 tcccggctgg cagtctctcg ggtccagcca gagccggctg ggctccctcg actgttccca
     8221 ctcattctcc agccggaccg atctcaatca ctggaatggt gctgggctgt cgggaactaa
     8281 ctgtggagac cttcacggca cttcactctg ggggaccccg cattattcca caagcctgtg
     8341 gggtccccca agcagcagcg acccccgagg aattagcagc ccatctccca ttaacgcttt
     8401 tctttctgtt gaccacctgg gtgggggtgg agagtccatg taactcgagt ctagagggcc
     8461 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     8521 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     8581 aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg
     8641 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     8701 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     8761 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     8821 acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc
     8881 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     8941 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
     9001 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
     9061 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
     9121 caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg
     9181 acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg
     9241 cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa
     9301 ggatcgctcg cggctcttac cagccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9361 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9421 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9481 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9541 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     9601 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9661 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9721 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9781 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     9841 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9901 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9961 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    10021 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    10081 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10141 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10201 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10261 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10321 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10381 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10441 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10501 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10561 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10621 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10681 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10741 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10801 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10861 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10921 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10981 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    11041 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    11101 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    11161 aataggcgta tcacgaggcc ctttcgt
//
