LOCUS       sequence-289294-       11223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6Biso1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 11223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pAc5.1B-EGFP-HsTNRC6Biso1_L
FEATURES             Location/Qualifiers
     source          1..11223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8508..8549
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8559..8576
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8599..8616)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8649..8783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8673..8692)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8727..8746
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9405..9993)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9485..9504)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10164..10955,10956..11024))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10787..10806
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11025..11129)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11197..11215
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgatgagag agaaggagca agaaagggaa gaacagttaa tggaagacaa
     3361 gaaaaggaag aaagaggata aaaagaaaaa agaagccact cagaaggtca cggaacaaaa
     3421 aaccaaagtg cccgaagtga cgaaaccaag tttaagccaa ccaacggccg ccagcccaat
     3481 tggcagctct ccatcgccac cagtcaatgg tggcaacaat gccaaaaggg tggcagtgcc
     3541 gaacggacaa ccgccaagcg ccgcccgcta catgcctcgg gaggtgccgc cgcgattccg
     3601 ttgccagcag gaccacaaag tgttactaaa acgtgggcag ccccctccac cgtcctgcat
     3661 gctccttggg ggtggggcag ggcctcctcc ctgcacagca cctggagcaa acccaaacaa
     3721 cgcacaagtg acaggagcgc tgctgcagag tgagagtggg actgcgccag actcaaccct
     3781 tggaggtgct gctgcttcaa attatgcaaa ttccacttgg ggctcgggag cctcctccaa
     3841 caacggcacc tcccccaacc caattcacat ctgggacaag gtgattgtag acgggtctga
     3901 catggaagag tggccttgta ttgccagcaa agacactgaa tcttcttccg aaaacaccac
     3961 cgataacaac agtgcctcga accctggctc tgagaagagc actctgccag gaagcaccac
     4021 tagtaacaaa ggaaaaggga gccagtgcca gtctgcaagt tctgggaacg aatgtaatct
     4081 tggggtctgg aaatctgacc ctaaggctaa atctgttcaa tcttccaact ctactacaga
     4141 gaacaacaat ggactaggaa attggaggaa tgtgagtggt caggatagaa ttggacctgg
     4201 ctctggcttc agcaacttta acccaaatag caacccatct gcctggccag cactggtcca
     4261 agaaggaact tctaggaaag gggcattgga aacagataat agtaattcca gtgcacaggt
     4321 tagcacagta ggtcagacat ccagggaaca gcagtcaaag atggaaaatg cgggtgttaa
     4381 ttttgttgtc tctggcagag aacaggctca aattcataac actgatggac caaaaaatgg
     4441 aaacactaac tccttgaact taagttcacc aaaccccatg gagaataagg gaatgccctt
     4501 tggaatgggc ttggggaaca cctccaggag cactgatgcc ccttcacaaa gcactggaga
     4561 tcgaaagact gggagtgttg gatcttgggg tgcagctagg gggccttctg gaactgacac
     4621 agtctctgga caaagcaatt ctggaaacaa tgggaacaat ggaaaagaga gagaggactc
     4681 ctggaaagga gcttctgttc agaaatcaac tgggtcaaaa aatgactctt gggacaacaa
     4741 taacaggtct acgggtgggt cctggaactt tggcccccag gactctaatg acaacaaatg
     4801 gggtgaaggg aacaaaatga catctggggt ctctcaggga gaatggaaac agccgactgg
     4861 gtctgatgag ttgaaaattg gagaatggag tggtccaaac caaccaaatt ctagcactgg
     4921 agcatgggac aatcaaaagg gccaccccct ccctgaaaac caaggcaatg cccaggctcc
     4981 ctgttgggga agatcttcca gctccacagg aagtgaagtt ggaggtcaaa gcactggaag
     5041 caaccacaaa gcaggaagta gtgacagtca taactctggc cgtcggtcgt acaggcccac
     5101 acatcctgat tgtcaggctg tcttgcagac tcttttgagc cgaactgatt tggaccccag
     5161 ggtgctctca aacactggct ggggccaaac tcaaattaag caggacacag tgtgggacat
     5221 tgaagaggtg ccaaggcctg aggggaaatc tgacaaagga actgaggggt gggagagcgc
     5281 tgccacacag accaagaact cagggggctg gggagatgca cccagccaaa gcaatcaaat
     5341 gaagtctgga tggggggagc tctcagcctc tacagagtgg aaagacccca agaacacagg
     5401 aggctggaat gactacaaga acaacaactc ttccaactgg ggaggaggac gacctgatga
     5461 aaagacacct tcctcttgga atgagaatcc cagcaaggat caggggtggg gaggtggacg
     5521 ccagcccaat caaggatggt cttctggaaa gaatggttgg ggggaggaag tcgatcagac
     5581 aaaaaacagc aattgggaaa gttctgcaag taaacctgtg tctgggtggg gtgaaggagg
     5641 gcagaatgaa atcgggactt ggggtaatgg tggcaatgca agcctagctt caaaaggtgg
     5701 gtgggaggat tgcaaaagat ccccagcatg gaatgagacg ggccgacagc ccaattcctg
     5761 gaataaacaa caccaacagc agcagccccc acagcagccg ccgccaccac aaccagaggc
     5821 ttctggttcg tggggaggcc cacccccacc acctccaggc aacgttcgac cttccaattc
     5881 cagctggagc agcgggccac agcctgcaac acctaaggat gaggaaccca gtggttggga
     5941 agagccatcc ccacagtcaa ttagtcggaa aatggacatt gatgatggca cttcagcatg
     6001 gggagaccct aacagttata actacaagaa tgtgaatctg tgggataaga attcccaagg
     6061 gggcccagca cctcgagaac caaacctgcc caccccaatg accagtaaat cggcatcaga
     6121 ttccaaatct atgcaagacg gctgggggga gagtgacggg ccagtcacag gagctcgcca
     6181 tcccagctgg gaagaggagg aggatggagg agtctggaac accactggct ctcagggcag
     6241 tgcttcctcc cacaactcag caagctgggg acaaggagga aagaaacaaa tgaagtgctc
     6301 actcaaagga ggaaacaatg attcatggat gaatcctctt gccaaacagt tttcaaatat
     6361 gggattgctg agtcagactg aagataatcc aagcagcaaa atggatttgt ctgtaggaag
     6421 cctttcagat aaaaaatttg atgtggacaa gcgagcgatg aatctcgggg attttaatga
     6481 tatcatgagg aaggatcgat ctgggttccg tccacctaat tccaaagaca tgggaaccac
     6541 agatagtggg ccttattttg agaagggcgg tagtcatggt ttgtttggaa acagcacagc
     6601 acaatcgaga ggtctgcaca cacccgtgca gccactaaat tcttctccca gtctccgggc
     6661 gcaagtgcct ccccagttta tttcccccca ggtttctgcc tcaatgctca agcagtttcc
     6721 caacagtggc ctgagtccag gtcttttcaa tgtggggccc cagttatctc ctcaacaaat
     6781 tgccatgctg agccagcttc cacaaattcc ccagtttcag ttggcatgtc agcttctctt
     6841 gcagcagcag caacagcagc agttgttaca gaaccagaga aagatttctc aagctgtacg
     6901 ccaacagcaa gagcagcagc tggctcgaat ggtgagtgca ctgcagcagc agcagcagca
     6961 gcagcagagg cagccaggca tgaagcactc gccctctcat cctgttgggc ccaagccgca
     7021 tctggacaac atggtaccca acgcattgaa tgtggggctc ccagaccttc aaaccaaagg
     7081 gccaatacct ggatatggtt ctggcttcag ctctggcggc atggactatg gcatggttgg
     7141 tgggaaggag gctggaaccg agtctcgctt taaacagtgg acctccatga tggaggggct
     7201 gccctctgta gccacacagg aagccaatat gcacaaaaat ggcgctatag tggcccctgg
     7261 taaaacccgg ggagggtcac cgtacaacca gtttgatatc atccctggtg acacactggg
     7321 tggccatacg ggtcctgctg gtgatagctg gttacctgcc aaatctccac caacaaataa
     7381 aatcggaagt aaatccagca atgccagttg gcctccagaa ttccaaccag gagtgccatg
     7441 gaaaggtatc caaaacattg accctgaatc tgacccctat gtcaccccag gaagtgtgct
     7501 ggggggtaca gccacatctc ccattgtaga tactgaccac caactgctgc gggataacac
     7561 cacagggtct aattcttccc tcaacacctc gctgccttca cctggtgcct ggccctacag
     7621 tgcctctgac aactccttta ccaacgttca tagcacttca gcaaagttcc ctgattacaa
     7681 atcaacatgg tccccagatc ccataggaca caaccccact catctctcca acaagatgtg
     7741 gaaaaaccat atttcctcca ggaacactac accgctgccc cgcccacctc ctggtctgac
     7801 caaccccaaa ccatcatctc cctggagcag cacagcaccc cgatcagtca gggggtgggg
     7861 gacacaggac tcacggctcg cctcggcctc tacctggagt gatggtggct cagttcgtcc
     7921 tagttactgg ctggttcttc acaatctcac cccacagatt gatgggtcaa ccttgagaac
     7981 gatctgcatg cagcatggcc cactgctgac attccatctg aatctaaccc agggcactgc
     8041 cctgatccga tacagcacca aacaggaggc ggccaaggcc caaactgcac tgcacatgtg
     8101 tgtgttggga aacactacca tccttgctga gtttgccact gatgatgaag tcagccgctt
     8161 tctggcacaa gctcagcccc ctacacctgc agcaacccca agtgcgccag ctgcggggtg
     8221 gcagtcgctg gagaccggcc agaaccagtc agatcccgtg ggacctgctc tgaatctttt
     8281 tggtgggtcc actgggctcg ggcagtggag cagcagcgct ggtggcagca gcggggccga
     8341 tcttgctggc gcttcattgt gggggccccc aaactattct tctagcttat ggggagtccc
     8401 aacggtggaa gatccccata ggatgggcag ccctgctcct ttactacctg gtgaccttct
     8461 gggaggaggg tcggattcaa tctgatctag agggcccgcg gttcgaaggt aagcctatcc
     8521 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
     8581 tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct
     8641 cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     8701 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     8761 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     8821 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     8881 attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     8941 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     9001 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     9061 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     9121 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     9181 gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     9241 attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     9301 aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     9361 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9421 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9481 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9541 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     9601 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9661 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9721 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9781 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9841 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9901 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9961 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    10021 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    10081 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10141 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10201 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10261 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10321 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10381 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10441 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
    10501 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10561 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10621 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10681 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10741 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
    10801 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10861 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10921 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10981 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    11041 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    11101 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11161 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    11221 cgt
//
