LOCUS       sequence-289295-       10911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6Biso1_1-1619.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10911)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 10911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pAc5.1B-EGFP-HsTNRC6Biso1_1-1619_L
FEATURES             Location/Qualifiers
     source          1..10911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8196..8237
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8247..8264
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8287..8304)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8337..8471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8361..8380)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8415..8434
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9093..9681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9173..9192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9852..10643,10644..10712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10475..10494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10713..10817)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10885..10903
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgatgagag agaaggagca agaaagggaa gaacagttaa tggaagacaa
     3361 gaaaaggaag aaagaggata aaaagaaaaa agaagccact cagaaggtca cggaacaaaa
     3421 aaccaaagtg cccgaagtga cgaaaccaag tttaagccaa ccaacggccg ccagcccaat
     3481 tggcagctct ccatcgccac cagtcaatgg tggcaacaat gccaaaaggg tggcagtgcc
     3541 gaacggacaa ccgccaagcg ccgcccgcta catgcctcgg gaggtgccgc cgcgattccg
     3601 ttgccagcag gaccacaaag tgttactaaa acgtgggcag ccccctccac cgtcctgcat
     3661 gctccttggg ggtggggcag ggcctcctcc ctgcacagca cctggagcaa acccaaacaa
     3721 cgcacaagtg acaggagcgc tgctgcagag tgagagtggg actgcgccag actcaaccct
     3781 tggaggtgct gctgcttcaa attatgcaaa ttccacttgg ggctcgggag cctcctccaa
     3841 caacggcacc tcccccaacc caattcacat ctgggacaag gtgattgtag acgggtctga
     3901 catggaagag tggccttgta ttgccagcaa agacactgaa tcttcttccg aaaacaccac
     3961 cgataacaac agtgcctcga accctggctc tgagaagagc actctgccag gaagcaccac
     4021 tagtaacaaa ggaaaaggga gccagtgcca gtctgcaagt tctgggaacg aatgtaatct
     4081 tggggtctgg aaatctgacc ctaaggctaa atctgttcaa tcttccaact ctactacaga
     4141 gaacaacaat ggactaggaa attggaggaa tgtgagtggt caggatagaa ttggacctgg
     4201 ctctggcttc agcaacttta acccaaatag caacccatct gcctggccag cactggtcca
     4261 agaaggaact tctaggaaag gggcattgga aacagataat agtaattcca gtgcacaggt
     4321 tagcacagta ggtcagacat ccagggaaca gcagtcaaag atggaaaatg cgggtgttaa
     4381 ttttgttgtc tctggcagag aacaggctca aattcataac actgatggac caaaaaatgg
     4441 aaacactaac tccttgaact taagttcacc aaaccccatg gagaataagg gaatgccctt
     4501 tggaatgggc ttggggaaca cctccaggag cactgatgcc ccttcacaaa gcactggaga
     4561 tcgaaagact gggagtgttg gatcttgggg tgcagctagg gggccttctg gaactgacac
     4621 agtctctgga caaagcaatt ctggaaacaa tgggaacaat ggaaaagaga gagaggactc
     4681 ctggaaagga gcttctgttc agaaatcaac tgggtcaaaa aatgactctt gggacaacaa
     4741 taacaggtct acgggtgggt cctggaactt tggcccccag gactctaatg acaacaaatg
     4801 gggtgaaggg aacaaaatga catctggggt ctctcaggga gaatggaaac agccgactgg
     4861 gtctgatgag ttgaaaattg gagaatggag tggtccaaac caaccaaatt ctagcactgg
     4921 agcatgggac aatcaaaagg gccaccccct ccctgaaaac caaggcaatg cccaggctcc
     4981 ctgttgggga agatcttcca gctccacagg aagtgaagtt ggaggtcaaa gcactggaag
     5041 caaccacaaa gcaggaagta gtgacagtca taactctggc cgtcggtcgt acaggcccac
     5101 acatcctgat tgtcaggctg tcttgcagac tcttttgagc cgaactgatt tggaccccag
     5161 ggtgctctca aacactggct ggggccaaac tcaaattaag caggacacag tgtgggacat
     5221 tgaagaggtg ccaaggcctg aggggaaatc tgacaaagga actgaggggt gggagagcgc
     5281 tgccacacag accaagaact cagggggctg gggagatgca cccagccaaa gcaatcaaat
     5341 gaagtctgga tggggggagc tctcagcctc tacagagtgg aaagacccca agaacacagg
     5401 aggctggaat gactacaaga acaacaactc ttccaactgg ggaggaggac gacctgatga
     5461 aaagacacct tcctcttgga atgagaatcc cagcaaggat caggggtggg gaggtggacg
     5521 ccagcccaat caaggatggt cttctggaaa gaatggttgg ggggaggaag tcgatcagac
     5581 aaaaaacagc aattgggaaa gttctgcaag taaacctgtg tctgggtggg gtgaaggagg
     5641 gcagaatgaa atcgggactt ggggtaatgg tggcaatgca agcctagctt caaaaggtgg
     5701 gtgggaggat tgcaaaagat ccccagcatg gaatgagacg ggccgacagc ccaattcctg
     5761 gaataaacaa caccaacagc agcagccccc acagcagccg ccgccaccac aaccagaggc
     5821 ttctggttcg tggggaggcc cacccccacc acctccaggc aacgttcgac cttccaattc
     5881 cagctggagc agcgggccac agcctgcaac acctaaggat gaggaaccca gtggttggga
     5941 agagccatcc ccacagtcaa ttagtcggaa aatggacatt gatgatggca cttcagcatg
     6001 gggagaccct aacagttata actacaagaa tgtgaatctg tgggataaga attcccaagg
     6061 gggcccagca cctcgagaac caaacctgcc caccccaatg accagtaaat cggcatcaga
     6121 ttccaaatct atgcaagacg gctgggggga gagtgacggg ccagtcacag gagctcgcca
     6181 tcccagctgg gaagaggagg aggatggagg agtctggaac accactggct ctcagggcag
     6241 tgcttcctcc cacaactcag caagctgggg acaaggagga aagaaacaaa tgaagtgctc
     6301 actcaaagga ggaaacaatg attcatggat gaatcctctt gccaaacagt tttcaaatat
     6361 gggattgctg agtcagactg aagataatcc aagcagcaaa atggatttgt ctgtaggaag
     6421 cctttcagat aaaaaatttg atgtggacaa gcgagcgatg aatctcgggg attttaatga
     6481 tatcatgagg aaggatcgat ctgggttccg tccacctaat tccaaagaca tgggaaccac
     6541 agatagtggg ccttattttg agaagggcgg tagtcatggt ttgtttggaa acagcacagc
     6601 acaatcgaga ggtctgcaca cacccgtgca gccactaaat tcttctccca gtctccgggc
     6661 gcaagtgcct ccccagttta tttcccccca ggtttctgcc tcaatgctca agcagtttcc
     6721 caacagtggc ctgagtccag gtcttttcaa tgtggggccc cagttatctc ctcaacaaat
     6781 tgccatgctg agccagcttc cacaaattcc ccagtttcag ttggcatgtc agcttctctt
     6841 gcagcagcag caacagcagc agttgttaca gaaccagaga aagatttctc aagctgtacg
     6901 ccaacagcaa gagcagcagc tggctcgaat ggtgagtgca ctgcagcagc agcagcagca
     6961 gcagcagagg cagccaggca tgaagcactc gccctctcat cctgttgggc ccaagccgca
     7021 tctggacaac atggtaccca acgcattgaa tgtggggctc ccagaccttc aaaccaaagg
     7081 gccaatacct ggatatggtt ctggcttcag ctctggcggc atggactatg gcatggttgg
     7141 tgggaaggag gctggaaccg agtctcgctt taaacagtgg acctccatga tggaggggct
     7201 gccctctgta gccacacagg aagccaatat gcacaaaaat ggcgctatag tggcccctgg
     7261 taaaacccgg ggagggtcac cgtacaacca gtttgatatc atccctggtg acacactggg
     7321 tggccatacg ggtcctgctg gtgatagctg gttacctgcc aaatctccac caacaaataa
     7381 aatcggaagt aaatccagca atgccagttg gcctccagaa ttccaaccag gagtgccatg
     7441 gaaaggtatc caaaacattg accctgaatc tgacccctat gtcaccccag gaagtgtgct
     7501 ggggggtaca gccacatctc ccattgtaga tactgaccac caactgctgc gggataacac
     7561 cacagggtct aattcttccc tcaacacctc gctgccttca cctggtgcct ggccctacag
     7621 tgcctctgac aactccttta ccaacgttca tagcacttca gcaaagttcc ctgattacaa
     7681 atcaacatgg tccccagatc ccataggaca caaccccact catctctcca acaagatgtg
     7741 gaaaaaccat atttcctcca ggaacactac accgctgccc cgcccacctc ctggtctgac
     7801 caaccccaaa ccatcatctc cctggagcag cacagcaccc cgatcagtca gggggtgggg
     7861 gacacaggac tcacggctcg cctcggcctc tacctggagt gatggtggct cagttcgtcc
     7921 tagttactgg ctggttcttc acaatctcac cccacagatt gatgggtcaa ccttgagaac
     7981 gatctgcatg cagcatggcc cactgctgac attccatctg aatctaaccc agggcactgc
     8041 cctgatccga tacagcacca aacaggaggc ggccaaggcc caaactgcac tgcacatgtg
     8101 tgtgttggga aacactacca tccttgctga gtttgccact gatgatgaag tcagccgctt
     8161 tctggcacaa tgatctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
     8221 tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct
     8281 gatcagcctc gactgtgcct tctaaggcct gagctcgctg atcagcctcg atcgaggatc
     8341 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     8401 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     8461 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     8521 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt
     8581 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     8641 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     8701 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     8761 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     8821 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     8881 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     8941 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     9001 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcca gcaaaaggcc
     9061 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     9301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     9541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9661 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9721 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9781 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9841 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9901 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9961 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10021 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10081 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10141 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
    10201 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10261 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10321 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10381 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10441 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
    10501 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10561 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10621 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10681 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10741 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10801 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
    10861 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg t
//
