LOCUS       sequence-289306-        7068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPABPC1_1-550-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pAc5.1B-lambdaN-DmPABPC1_1-550-siRNAres_L
FEATURES             Location/Qualifiers
     source          1..7068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             4353..4394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4404..4421
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4444..4461)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4494..4628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4518..4537)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4572..4591
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5250..5838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5330..5349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6009..6800,6801..6869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6632..6651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6870..6974)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7042..7060
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttctctatac gtcggtgatc
     2701 tcccacagga cgtcaacgaa tcgggacttt ttgacaagtt ctcgtctgct agtccagtgc
     2761 tgtccattcg tgtctgccgc gatgtgatta cccgtcgctc gttgggctat gcctacgtca
     2821 acttccagca gccagccgat gctgagcgtg ctttggacac catgaacttt gacctggttc
     2881 gcaacaagcc cattcgcatt atgtggtctc agcgtgatcc gtctcttcgt cgctccggtg
     2941 tgggcaacgt gttcatcaag aacttagata gggccatcga caacaaggca atctacgaca
     3001 ctttctccgc cttcggcaac attttaagct gcaaggtagc caccgatgaa aagggcaatt
     3061 cgaaaggata cggcttcgtc cacttcgaga ccgaggaggc cgcaaacacg tccatcgaca
     3121 aagtcaatgg catgttgctt aacggcaaga aggtctacgt gggtaagttc atcccgcgca
     3181 aggagcgcga gaaggagctg ggcgagaagg ctaagctctt caccaatgta tatgtgaaga
     3241 acttcactga ggatttcgac gatgaaaaac tgaaggaatt ctttgagccc tacggcaaga
     3301 taaccagcta caaggtcatg tccaaggagg atggcaagag caagggcttc ggattcgttg
     3361 cattcgagac aacagaggct gctgaggcgg ccgttcaggc tctcaatggc aaggacatgg
     3421 gagagggcaa gtccctgtac gtggctcgtg cccagaagaa ggccgaacgc cagcaggagc
     3481 tgaagcgcaa gttcgaggaa ctgaagcaga agcgtcacga gtcagtgttc ggcgttaact
     3541 tgtacgttaa gaacctggac gacacaatcg atgacgatcg tctgcgcatc gcgttctctc
     3601 catacggcaa catcacatcg gccaaggtca tgaccgatga ggagggtcgc tccaagggat
     3661 tcggcttcgt gtgcttcaat gccgcaagcg aggctacctg cgccgtcacc gagctgaacg
     3721 gtcgcgtcgt cggcagtaag ccgttgtacg ttgctttggc ccaaaggaag gaggagcgca
     3781 aggctcatct cgcctcgcag tacatgcgtc acatgaccgg catgcgtatg cagcagctgg
     3841 gacagatcta ccagcccaac gcggctagcg gcttctttgt gccgaccctt ccgtcaaatc
     3901 agcgtttctt cggttcccag gtggccactc agatgagaaa cacaccccgc tgggtgcccc
     3961 aggtgcgtcc cccagcagcc atacagggtg tccaggccgg agctgccgct gctggtggct
     4021 tccagggcac agccggtgcg gtgcccactc agttccgttc ggctgctgct ggcgctcgcg
     4081 gggcacagcc tcaggtgcag ggcacacacg ccgccgctgc tgcggctaac aacatgcgca
     4141 acactggagc acgcgctatt actggccagc aaactgcagc acccaacatg cagatccccg
     4201 gagcccaaat cgctggcggt gcccagcagc gtacttccaa ctataagtac acctcaaaca
     4261 tgcgtaatcc tccagtgcca cagttgcatc agacccagcc aattccacag caattgcaag
     4321 gaaactcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
     4381 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
     4441 cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc gaggatccag
     4501 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     4561 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     4621 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     4681 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagtcga
     4741 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     4801 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     4861 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
     4921 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc
     4981 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
     5041 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
     5101 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
     5161 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagccagca aaaggccagg
     5221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5281 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5401 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     5461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     5701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5941 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6001 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6061 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6121 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6181 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     6241 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     6301 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     6361 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     6421 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     6481 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     6541 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     6601 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     6661 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     6721 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     6781 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6841 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6961 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     7021 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt
//
