LOCUS       sequence-289309-        7092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPABPC1_del183-258-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: 
            pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L
FEATURES             Location/Qualifiers
     source          1..7092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             4377..4418
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4428..4445
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4468..4485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4518..4652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4542..4561)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4596..4615
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5274..5862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5354..5373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6033..6824,6825..6893))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6656..6675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6894..6998)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7066..7084
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttctctatac gtcggtgatc
     2701 tcccacagga cgtcaacgaa tcgggacttt ttgacaagtt ctcgtctgct agtccagtgc
     2761 tgtccattcg tgtctgccgc gatgtgatta cccgtcgctc gttgggctat gcctacgtca
     2821 acttccagca gccagccgat gctgagcgtg ctttggacac catgaacttt gacctggttc
     2881 gcaacaagcc cattcgcatt atgtggtctc agcgtgatcc gtctcttcgt cgctccggtg
     2941 tgggcaacgt gttcatcaag aacttagata gggccatcga caacaaggca atctacgaca
     3001 ctttctccgc cttcggcaac attttaagct gcaaggtagc caccgatgaa aagggcaatt
     3061 cgaaaggata cggcttcgtc cacttcgaga ccgaggaggc cgcaaacacg tccatcgaca
     3121 aagtcaatgg catgttgctt aacggcaaga aggtctacgt gggtaagttc atcccgcgca
     3181 aggagcgcga gaaggagctg ggcgagaagg ctaagctctt cgcccagaag aaggccgaac
     3241 gccagcagga gctgaagcgc aagttcgagg aactgaagca gaagcgtcac gagtcagtgt
     3301 tcggcgttaa cttgtacgtt aagaacctgg acgacacaat cgatgacgat cgtctgcgca
     3361 tcgcgttctc tccatacggc aacatcacat cggccaaggt catgaccgat gaggagggtc
     3421 gctccaaggg attcggcttc gtgtgcttca atgccgcaag cgaggctacc tgcgccgtca
     3481 ccgagctgaa cggtcgcgtc gtcggcagta agccgttgta cgttgctttg gcccaaagga
     3541 aggaggagcg caaggctcat ctcgcctcgc agtacatgcg tcacatgacc ggcatgcgta
     3601 tgcagcagct gggacagatc taccagccca acgcggctag cggcttcttt gtgccgaccc
     3661 ttccgtcaaa tcagcgtttc ttcggttccc aggtggccac tcagatgaga aacacacccc
     3721 gctgggtgcc ccaggtgcgt cccccagcag ccatacaggg tgtccaggcc ggagctgccg
     3781 ctgctggtgg cttccagggc acagccggtg cggtgcccac tcagttccgt tcggctgctg
     3841 ctggcgctcg cggggcacag cctcaggtgc agggcacaca cgccgccgct gctgcggcta
     3901 acaacatgcg caacactgga gcacgcgcta ttactggcca gcaaactgca gcacccaaca
     3961 tgcagatccc cggagcccaa atcgctggcg gtgcccagca gcgtacttcc aactataagt
     4021 acacctcaaa catgcgtaat cctccagtgc cacagttgca tcagacccag ccaattccac
     4081 agcaattgca aggaaagaat tctgagaagc tcattgcctc gttgctggcc aacgccaagc
     4141 cgcaggagca gaaacagatc ctgggcgagc gtctgtatcc gatgattgaa catatgcacg
     4201 ccaatttggc tggtaagatc accggcatgt tgttggagat cgaaaactct gagctcttgc
     4261 acatgatcga ggatcaggag gcccttaagg ccaaggtgga ggaggctgtg gccgtgcttc
     4321 aggtgcaccg cgtcaccgag cccgccaact cgagtctaga gggcccgcgg ttcgaaggta
     4381 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
     4441 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct
     4501 gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca
     4561 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4621 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4681 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4741 gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     4801 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     4861 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     4921 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     4981 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     5041 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     5101 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     5161 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     5221 cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5281 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5461 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5701 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5761 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5821 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5881 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5941 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6001 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     6361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     6661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7021 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7081 aggccctttc gt
//
