LOCUS       sequence-289312-        9909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pEGFP-C1-HsTNRC6C_L
FEATURES             Location/Qualifiers
     source          1..9909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3627..3644
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6697..6818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6734..6753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6788..6807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6825..7280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6962..6983)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7174..7193
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7307..7411
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7413..7770
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7413..7604
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7436..7456)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7621..7756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7805..8599
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7805..8599
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7859..7878)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8469..8488
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8787..8806)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8831..8878
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9207..9795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9696..9715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagga gctacaggga gtgcccaggg caacttcact
     1381 ggacatacca agaagacaaa tggcaataat ggcaccaatg gcgcactcgt ccaaagccct
     1441 tctaatcaga gtgcccttgg agcaggggga gcgaacagta atggaagtgc ggccagagtg
     1501 tggggtgtag ccacaggctc cagctctggc ctggctcact gctctgtcag tggtggggat
     1561 ggaaaaatgg acactatgat tggagatggg agaagtcaga attgctgggg tgcttccaac
     1621 tccaatgctg gcattaatct taaccttaat cctaatgcca acccagctgc ctggcctgta
     1681 cttggacatg aaggaaccgt ggcgacaggc aacccttcca gtatttgcag tccagtcagt
     1741 gccataggtc aaaatatggg caaccagaac gggaacccaa caggcacttt aggtgcttgg
     1801 ggaaacttgc tgccacaaga gagcacagaa ccacaaacgt ccacttctca gaatgtgtct
     1861 ttcagcgcac aacctcagaa ccttaacact gatggaccaa ataacactaa ccccatgaac
     1921 tcttcaccca accctatcaa tgcaatgcag acaaatggac tgccaaactg gggcatggct
     1981 gttggtatgg gggccatcat cccgccccac ctgcaaggcc ttcctggtgc taatggatca
     2041 tcagtttctc aagtcagtgg gggcagtgct gaaggaataa gcaattctgt gtggggactg
     2101 tccccaggta accctgccac aggaaatagc aattctgggt tcagtcaggg gaatggagac
     2161 actgtgaact cagcattaag tgctaaacaa aatggatcca gcagtgctgt gcaaaaggaa
     2221 ggaagtggag gaaatgcttg ggattcagga cctcctgctg gtcctggaat actcgcctgg
     2281 ggaaggggca gtggcaacaa tggcgttggt aatatccatt caggagcttg gggccacccc
     2341 agccgaagca cctctaacgg tgtgaatggg gaatggggaa agcccccaaa ccagcattcc
     2401 aacagtgaca tcaatgggaa aggatcaaca gggtgggaga gtcctagtgt caccagccag
     2461 aaccctaccg tacagcctgg tggtgaacac atgaactcct gggccaaagc ggcatcttct
     2521 ggaactacag caagtgaagg aagtagtgat ggttctggca accacaatga aggaagcact
     2581 gggagggaag gaacgggaga aggccgaagg cgagataaag ggattataga ccaagggcac
     2641 atccagttgc caaggaatga tcttgaccca agagttctgt ctaatactgg ttggggacag
     2701 actcctgtaa agcaaaacac tgcctgggaa tttgaagaat cccctaggtc tgaaaggaaa
     2761 aatgacaatg ggacagaggc ctggggttgt gcagctactc aggcttcaaa ctcagggggg
     2821 aagaacgatg ggtccatcat gaacagtaca aatacctctt cagtatctgg gtgggtcaac
     2881 gcgccacctg ccgctgtgcc agcaaacaca ggttggggag acagcaacaa caaagcgcca
     2941 agtggcccgg gggtttgggg ggactcgata agctctactg ctgttagtac tgctgctgct
     3001 gccaagagtg gccatgcttg gagtggggcc gcaaatcagg aggacaagtc acccacctgg
     3061 ggtgagcctc caaagcccaa atcccaacac tggggagatg gacaaagatc aaatccagcc
     3121 tggagtgcag gagggggaga ttgggcagat tcatcgtctg tccttggaca cttgggggat
     3181 gggaaaaaaa atggatctgg atgggatgct gacagtaata ggtcagggtc tggttggaat
     3241 gacaccacga gatctgggaa cagtggctgg ggcaacagca caaatacaaa ggccaatcca
     3301 ggtacaaact ggggggagac tttaaaacct ggcccccaac agaactgggc tagcaaaccc
     3361 caagacaaca atgtgagtaa ctggggagga gctgcttctg tgaaacagac aggaacaggg
     3421 tggatcgggg ggccggtacc ggtcaaacag aaggacagca gtgaagcaac tggctgggaa
     3481 gaaccctctc caccgtccat tcgccgcaaa atggaaattg atgatggtac ctcagcttgg
     3541 ggggacccaa gcaactataa caataaaact gtaaacatgt gggatagaaa caacccggtc
     3601 atccagagca gtaccacgac caataccacc accaccacca ccactaccac gagcaacacc
     3661 acacacaggg tcgagacgcc gcccccgcac caggctggta ctcagctgaa tcgatcaccg
     3721 ttgcttggtc caggtaggaa agtttcatca ggctggggag aaatgcctaa tgttcactca
     3781 aagactgaaa actcttgggg agaaccatcc tccccttcta ccctggtgga taatggcaca
     3841 gcagcatggg ggaagccacc cagcagtggc agcgggtggg gagatcaccc tgccgagccg
     3901 ccggtggcat ttggaagagc tggcgcacct gttgctgcct cagccctgtg caaaccagct
     3961 tcaaaatcta tgcaagaagg ctggggcagt ggtggggatg aaatgaacct cagtaccagc
     4021 cagtgggagg atgaagaagg ggacgtgtgg aataatgctg cttcccaaga aagcacctcc
     4081 tcctgcagct cctgggggaa cgcccccaaa aaaggacttc aaaagggcat gaagacgtct
     4141 ggcaagcagg atgaggcctg gatcatgagc cggctgatca aacaactcac agacatgggc
     4201 ttcccgagag agccagctga ggaggccttg aagagtaaca atatgaatct tgatcaggcc
     4261 atgagcgctc tgctggaaaa gaaggtggac gtggacaagc gtgggctggg agtgaccgac
     4321 cataatggaa tggccgccaa gcccctcggc tgccgcccgc caatctccaa agagtcttcc
     4381 gtggaccgcc ccacctttct tgacaaggat ggcggcctcg tggaagagcc cacgccttca
     4441 ccgttcttgc cttccccaag cctgaagctc cccctttcac acagtgcact ccccagtcag
     4501 gccctgggtg ggattgcctc cgggctgggc atgcaaaact tgaattcttc tagacagata
     4561 ccgagtggca atctgggtat gtttggcaat agtggagcag cacaagccag gaccatgcag
     4621 cagccgccac agccaccagt gcagcctctt aactcttccc agcccagtct ccgtgctcaa
     4681 gtgcctcagt ttctatcccc tcaggttcaa gcacagcttt tgcagtttgc agcaaaaaac
     4741 attggtctca accctgcact attaacctcg ccaattaatc ctcaacatat gacgatgttg
     4801 aaccagctct atcagctgca gctggcatac caacgtttac aaatccagca gcagatgtta
     4861 caggcccagc gtaatgtgtc cggatccatg agacaacagg agcagcaagt tgcgcgcaca
     4921 atcactaatc tgcagcagca gatccagcag caccagcgcc agctggccca ggccctgctc
     4981 gtgaagcagc caccaccgcc accgcccccg ccgcacctgt ctctgcaccc ctctgcaggc
     5041 aaatcggcca tggacagctt cccctcgcac ccacagactc ccggcctacc tgacctgcag
     5101 accaaagagc agcagtcttc acccaacacc tttgctcctt accctctcgc tggactgaac
     5161 ccaaacatga atgtcaacag catggacatg accggtggct tgtcggtgaa ggacccatcc
     5221 cagtcccagt cacgcctccc ccagtggacg caccccaact ccatggataa cttgcccagt
     5281 gccgcttccc ccctggagca gaaccctagc aagcatggtg ctatccctgg aggtctaagc
     5341 attgggcctc caggtaagtc ctccattgat gactcctatg gccggtacga tttaatccag
     5401 aacagtgagt caccagccag tcctcccgta gctgttcccc atagctggtc acgtgccaaa
     5461 tctgacagtg ataaaatctc aaatggctct agcatcaact ggcccccaga attccatccg
     5521 ggagttccat ggaaaggact gcagaatatt gaccctgaga atgaccctga cgtcactcct
     5581 ggcagtgtcc ccactgggcc taccatcaac accaccatcc aggatgtcaa ccgctacctc
     5641 ctcaagagtg gaggtaaact gtcagacatc aaatcgacgt ggtcctctgg ccctacctcc
     5701 cacacgcaag cctctctgtc tcatgaacta tggaaggtgc ccagaaacag tactgcaccc
     5761 acgaggccac ctccagggtt aaccaatccc aagccctcct ccacctgggg tgccagcccc
     5821 ctcggctgga ccagctccta ctcctcgggt tctgcctgga gcaccgacac ctcaggaaga
     5881 accagcagct ggctcgttct tcgaaacctc actccccaga ttgatggttc tacactgcgg
     5941 acattgtgtt tgcaacatgg gcctcttatc acattccacc tgaatctgac tcaaggcaat
     6001 gctgtggtcc ggtacagctc caaggaggag gctgccaagg cccagaagtc tctgcacatg
     6061 tgcgtcctgg gaaacactac catcctggcc gagttcgctg gtgaagaaga agtgaatcgc
     6121 ttcttagccc aaggccaggc gctgccaccc acttccagct ggcagtccag cagcgcgtcc
     6181 agccagccgc ggctcagcgc agcgggcagc tcccatggcc tggtacgcag cgacgctggc
     6241 cactggaacg ccccgtgcct gggtggcaag gggagcagtg agctgctgtg gggcggggtg
     6301 ccccagtact ccagcagcct gtggggcccg cccagcgccg acgacagcag ggtgataggc
     6361 agccccacgc cgctaaccac cctgctgcct ggggacctgc tcagcgggga gtccctgtag
     6421 gcgctgccat catcagcacc aggagagccg acccctcccg ggacccctcc cggctgggcg
     6481 gccccacaga cccgctggaa cccagcagcg gccgcttccc tttagtgagg gttaatgctt
     6541 cgagcagtcg acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc
     6601 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     6661 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     6721 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6781 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg taagcgttaa
     6841 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     6901 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     6961 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     7021 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     7081 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     7141 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     7201 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     7261 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7381 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     7441 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7501 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7561 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7621 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7681 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     7741 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     7801 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     7861 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     7921 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     7981 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     8041 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     8101 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     8161 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     8221 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     8281 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     8341 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     8401 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8461 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     8521 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     8581 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     8641 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8701 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8761 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     8821 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     8881 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     8941 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     9001 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     9061 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9121 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9241 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9301 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     9361 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9421 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9541 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9721 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9781 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9841 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9901 gccatgcat
//
