LOCUS       sequence-289338-        3490 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of Dm7SL-RNA-long.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Igreja C, Izaurralde E
  TITLE     CUP promotes deadenylation and inhibits decapping of mRNA targets.
  JOURNAL   Genes Dev. 2011 Sep 15;25(18):1955-67. doi: 10.1101/gad.17136311.
  PUBMED    21937713
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2011-09-15- 15"; volume: "25"; 
            issue: "18"; pages: "1955-67"
COMMENT     Sequence Label: pAc5.1-Dm7SL-RNA-long_M
FEATURES             Location/Qualifiers
     source          1..3490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(896..913)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(970..989)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1024..1043
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(1741..2329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1821..1840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2500..3291,3292..3360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3123..3142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3361..3465)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtctaac ttcccctttg tgttagaata aagttagctg tatctaaaaa tatgaattac
       61 aaaagtaaca aaaagggaca atgacagata acacgttgta taaatgtcag tgtgtactga
      121 ccctcgaaga tcaaagaatg aggaccctcg gtttcctcct attatccctc attggtgggc
      181 gagtactgta cacggcatat gtgcagccga ttcctatggt gccttgtatg gtagcgagtt
      241 gagtgctggc ttatttcatc agtgtaacgc ccagttgatt tgacacccgt acatcaaaat
      301 aggagctaca atagtcatca caaagagtga accgaacatt tcggccagta ggtattttcc
      361 tttgggcaaa tgcttctatc aacggcagtt ggcaacgcgg tcgccgagac tgcttcgtgt
      421 cgatggagtg gtgcgccgat aaagcagttc cccccaaacc gaatagaagt cggaggcgtg
      481 tggctgagtt ttagacgaac gactttcgac tggaaggttg gcagcttctg taatcacgct
      541 tctgtgaggt ctgattgtgg gatggcctga ggctgggatc tactgcgtag cggaccagct
      601 catgttgacg gaacgtccgc actaagcttg ccatcaatat gggtgccatg gaggagtccg
      661 tggcattcag gttggctaag gagggatgaa ccgggccagg ggtgaaaacc agcagccaag
      721 agttcccgtg gtaggcagta gtgggatagc gtaccggagt ggactgccgt tatcagccca
      781 accgatatgg ttggaccaca atctactaaa ggtacccgct gatcaccagt accctcgata
      841 aatgtcaacc agtaccctcg ataaatgtca accagtaccc tcgataaatg tcaagcctcg
      901 actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata
      961 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     1021 tgtgaaattt gtgatgctat tgctttattt gtagctagcg atatggttgg accacaatct
     1081 tttttgatgc cgaaaacata atttgcattt ttctttctat tctttttgcc tatagcaaat
     1141 gaattgggct attttttttt aattattaaa tcttaaataa aaaatcaatt tataatttcg
     1201 ttattgcctt tcgaatcgac acttccgtcg accgatgccc ttgagagcct tcaacccagt
     1261 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
     1321 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
     1381 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
     1441 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
     1501 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
     1561 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
     1621 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
     1681 cgcggctctt accagccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1921 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2161 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
     2821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     3121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3481 agtgccacct
//
