LOCUS       sequence-289339-        3444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of Dm7SL-RNA-short.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Igreja C, Izaurralde E
  TITLE     CUP promotes deadenylation and inhibits decapping of mRNA targets.
  JOURNAL   Genes Dev. 2011 Sep 15;25(18):1955-67. doi: 10.1101/gad.17136311.
  PUBMED    21937713
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2011-09-15- 15"; volume: "25"; 
            issue: "18"; pages: "1955-67"
COMMENT     Sequence Label: pAc5.1-Dm7SL-RNA-short_M
FEATURES             Location/Qualifiers
     source          1..3444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(850..867)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(924..943)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     978..997
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(1695..2283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1775..1794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2454..3245,3246..3314))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3077..3096
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3315..3419)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtctaac ttcccctttg tgttagaata aagttagctg tatctaaaaa tatgaattac
       61 aaaagtaaca aaaagggaca atgacagata acacgttgta taaatgtcag tgtgtactga
      121 ccctcgaaga tcaaagaatg aggaccctcg gtttcctcct attatccctc attggtgggc
      181 gagtactgta cacggcatat gtgcagccga ttcctatggt gccttgtatg gtagcgagtt
      241 gagtgctggc ttatttcatc agtgtaacgc ccagttgatt tgacacccgt acatcaaaat
      301 aggagctaca atagtcatca caaagagtga accgaacatt tcggccagta ggtattttcc
      361 tttgggcaaa tgcttctatc aacggcagtt ggcaacgcgg tcgccgagac tgcttcgtgt
      421 cgatggagtg gtgcgccgat aaagcagttc cccccaaacc gaatagaagt cggaggcgtg
      481 tggctgagtt ttagacgaac gactttcgac tggaaggttg gcagcttctg taatcacgct
      541 tctgtgaggt ctgattgtgg gatggcctga ggctgggatc tactgcgtag cggaccagct
      601 catgttgacg gaacgtccgc actaagcttg ccatcaatat gggtgccatg gaggagtccg
      661 tggcattcag gttggctaag gagggatgaa ccgggccagg ggtgaaaacc agcagccaag
      721 agttcccgtg gtaggcagta gtgggatagc gtaccggagt ggactgccgt tatcagccca
      781 accgatatgg ttggaccaca atctactaaa ggtacccgct gatcaccagt accctcgata
      841 aatgtcaagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
      901 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      961 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtagct agcgatatgg
     1021 ttggaccaca atcttttttg atgccgaaaa cataatttgc atttttcttt ctattctttt
     1081 tgcctatagc aaatgaattg ggctattttt ttttaattat taaatcttaa ataaaaaatc
     1141 aatttataat ttcgttattg cctttcgaat cgacacttcc gtcgaccgat gcccttgaga
     1201 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     1261 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     1321 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     1381 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     1441 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     1501 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     1561 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     1621 cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc
     1681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1861 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     1921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2101 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2281 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2341 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2401 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2461 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2521 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2581 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2641 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2701 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2761 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2821 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2881 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2941 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3001 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3061 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3121 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3181 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3241 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3301 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3361 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3421 acatttcccc gaaaagtgcc acct
//
