LOCUS       sequence-289349-        7821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCup_1-779.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Igreja C, Izaurralde E
  TITLE     CUP promotes deadenylation and inhibits decapping of mRNA targets.
  JOURNAL   Genes Dev. 2011 Sep 15;25(18):1955-67. doi: 10.1101/gad.17136311.
  PUBMED    21937713
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2011-09-15- 15"; volume: "25"; 
            issue: "18"; pages: "1955-67"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmCup_1-779_M
FEATURES             Location/Qualifiers
     source          1..7821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5106..5147
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5157..5174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5197..5214)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5247..5381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5271..5290)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5325..5344
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6003..6591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6083..6102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6762..7553,7554..7622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7385..7404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7623..7727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7795..7813
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggaccct atgaaagagg aggcgatcga
     2761 attgaatggc catgctatgc aaatggccga agctgagcaa gaaaatggcg ctggcgccct
     2821 gaaaattgcc accaatgcgg gtgcaaccga tcgtccagcg catcagcagc ttccgctgcc
     2881 agtcgaagat cagcaggatg aggtgctgac ccccgctgag aagggcaagt tcgagtaccc
     2941 gccccctcca ccgccaccaa ctccagtcca ggctccgcta gccaccaagg ccaccgctct
     3001 gaatgccagt caagagcatg acgacgacga ggccaactcc gagaagtggg aggatccgtg
     3061 cgctccgccc ccgcctcctc cgctgccgac tagcgctttc ctggccaccg gactgggcta
     3121 cctcaagctg cccgccttca agctgaagga cgcactggag aaggccatca ccaagttgga
     3181 ggccaacaag cggacgctaa aggccagccc tgagtcttct cgttccatca aaaacaagaa
     3241 tgtggtggcc ctggagatgt tacccaggcg ctcaaaccca gaaacgatcg gcgatggatc
     3301 catgttggca tccacatcca cggctgttat gctgcagacc aaaaagcccg ccgttattgt
     3361 ggagatggag aggcgttgta agatcatcaa tttactggcc aaacagaatc aaatcctcga
     3421 atccatttcg ggggaggcca ttccgatgca tggaccctcg aagcatctgc acgaggacga
     3481 gggtctgacc ctgcaagttc tctcggcccg agcatccaca ccgtacacac agccctccag
     3541 catgttgtcc tgcacggccg ttagttgtga cctggagcac gattcgccgc ggaagcaagt
     3601 ggccagcaag gaggccgtgc cggagcagca gtctagtcag gtgcagcaaa agcgtccacc
     3661 cagcacaggc attcacaagc caggaagtct gcgagcaccc aaggctgttc gtcctactac
     3721 tgctccggtg gtcagcagca agccggtcaa gagctatacc cgctcccgcc tgatggacat
     3781 tcgcaacggg atgttcaatg ccctgatgca caggtccaag gagagctttg tgatgcctcg
     3841 catcgccacc tgcgacgaca ttgagttgga gggaaggctt cgccgcatga atatttggcg
     3901 cacctcggac ggcacaagat tccggacccg ctccaccaca gctaacttaa acatgaacaa
     3961 caacaacaac aatgagtgca tgcctgcctt cttcaaaaac aaaaataagc cgaacctaat
     4021 cagtgacgag tccatcatcc agagccaacc gccccagccg caaacggagt tccaagatcc
     4081 ggccattgtt aaccaaagac gcattggcag cggtcgctta aaccattcca agtggggtta
     4141 caacgacgaa gactaccact cgtaccacaa tggcaagtcg cagcatatgg aggaggtcaa
     4201 ctcgaagaac agcaaaaaca tgacggtgct gcagttcttc gacaacggcg agatcagcag
     4261 tcagcctcaa agacggccga acactcctgt gatggggatg tctatcaatc gatccgagaa
     4321 cgacaccctg cactcgaacg agtccagtga agatttgagt cgcgccaacg agaactatgt
     4381 aaagcgcgta atgtctggat tcctggttgt atcgaagccc aagtcacgag atgtcgagga
     4441 tagacatcac cggcggtaca ggaaccagaa cgaggagcct gagtggttta gctgtggacc
     4501 cacgtctaga ctggacacta tagagttgtg tgggttcgat gaggacgagg agaaaatgct
     4561 aaaggagggc aacaaaaacc acggattagg cgaaacagag cgagaaacgt caaaacaaaa
     4621 gatggaccat aagtacaaat ggacgcatgc ggagccgatg ggtcgaagca aatacatgcc
     4681 caaacacgac actaacaata atcataacgt ggagaacatg aataatgtta tggctaccga
     4741 gcatcagcaa caaaaggaag agaaacgccc aggcagtggc cgttcgtttc agtttgataa
     4801 gttcaatcaa agccaacaga attacgagag tagcagttat gttaatcacc aacagccgcc
     4861 gcaaacgcag cctcagcaaa tgcagcagca atcgaacaca aacacgaaca actctaaatt
     4921 catgtcattc tttgccaatg aggggaactc ctcgtcttct tcgctgaacg agtttttcaa
     4981 gcaggccatt aaccagggtc atggcaacaa tccggagcag cccaaatcgt tgggccacat
     5041 cggacagatg ccttcggtgg atcagcttaa gcggccgctc gagtctagag ggcccgcggt
     5101 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc
     5161 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaaggcct
     5221 gagctcgctg atcagcctcg atcgaggatc cagacatgat aagatacatt gatgagtttg
     5281 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     5341 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     5401 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     5461 acaaatgtgg tatggctgat tatgatcagt cgaccgatgc ccttgagagc cttcaaccca
     5521 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     5581 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     5641 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     5701 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     5761 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     5821 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     5881 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     5941 ctcgcggctc ttaccagcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6001 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6061 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6121 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6181 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6241 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6301 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6361 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6421 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6481 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6541 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6601 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6661 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6721 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6781 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6841 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6901 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6961 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7021 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
     7081 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7141 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7201 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7261 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7321 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     7381 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7441 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7501 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7561 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7621 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7681 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7741 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7801 cgtatcacga ggccctttcg t
//
