LOCUS       sequence-289353-        7633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCup_418-1132.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Igreja C, Izaurralde E
  TITLE     CUP promotes deadenylation and inhibits decapping of mRNA targets.
  JOURNAL   Genes Dev. 2011 Sep 15;25(18):1955-67. doi: 10.1101/gad.17136311.
  PUBMED    21937713
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2011-09-15- 15"; volume: "25"; 
            issue: "18"; pages: "1955-67"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmCup_418-1132_M
FEATURES             Location/Qualifiers
     source          1..7633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4918..4959
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4969..4986
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5009..5026)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5059..5193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5083..5102)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5137..5156
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5815..6403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5895..5914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6574..7365,7366..7434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7197..7216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7435..7539)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7607..7625
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgcctgcc ttcttcaaaa acaaaaataa
     2761 gccgaaccta atcagtgacg agtccatcat ccagagccaa ccgccccagc cgcaaacgga
     2821 gttccaagat ccggccattg ttaaccaaag acgcattggc agcggtcgct taaaccattc
     2881 caagtggggt tacaacgacg aagactacca ctcgtaccac aatggcaagt cgcagcatat
     2941 ggaggaggtc aactcgaaga acagcaaaaa catgacggtg ctgcagttct tcgacaacgg
     3001 cgagatcagc agtcagcctc aaagacggcc gaacactcct gtgatgggga tgtctatcaa
     3061 tcgatccgag aacgacaccc tgcactcgaa cgagtccagt gaagatttga gtcgcgccaa
     3121 cgagaactat gtaaagcgcg taatgtctgg attcctggtt gtatcgaagc ccaagtcacg
     3181 agatgtcgag gatagacatc accggcggta caggaaccag aacgaggagc ctgagtggtt
     3241 tagctgtgga cccacgtcta gactggacac tatagagttg tgtgggttcg atgaggacga
     3301 ggagaaaatg ctaaaggagg gcaacaaaaa ccacggatta ggcgaaacag agcgagaaac
     3361 gtcaaaacaa aagatggacc ataagtacaa atggacgcat gcggagccga tgggtcgaag
     3421 caaatacatg cccaaacacg acactaacaa taatcataac gtggagaaca tgaataatgt
     3481 tatggctacc gagcatcagc aacaaaagga agagaaacgc ccaggcagtg gccgttcgtt
     3541 tcagtttgat aagttcaatc aaagccaaca gaattacgag agtagcagtt atgttaatca
     3601 ccaacagccg ccgcaaacgc agcctcagca aatgcagcag caatcgaaca caaacacgaa
     3661 caactctaaa ttcatgtcat tctttgccaa tgaggggaac tcctcgtctt cttcgctgaa
     3721 cgagtttttc aagcaggcca ttaaccaggg tcatggcaac aatccggagc agcccaaatc
     3781 gttgggccac atcggacaga tgccttcggt ggatcagctg gaggccaaat ggcgtcgaaa
     3841 ctcactgaac aatgttggcg agactgccaa caagcagact gataacttcc agaagctaat
     3901 tgggtctctt tcttcggcta agccacagtc acaggcggtt ggctatgatg ccatctccaa
     3961 ctttataatg cagcaacagc agtaccagca gcagcaacag aagcagcacc tcatcataca
     4021 gcagcagcag caacagaccg cctttttggc gagcctgcag ctgaaggcga tcctcggccg
     4081 ggccgacacc caattgctac tgctccgtct gactaaaggg gaaatatcca agcacggatt
     4141 gctggtgcag ttggccaacc cacgcttgac ggacatggat cgcgaggcca tcactgctgt
     4201 attgcagttc accaacaccc agcagcagca gcagcagcac aagcagcagc tggacatgct
     4261 ctcaagcacg gtcatcgcca gccagttgca gaatctccac aacttggcca ttgtccagca
     4321 gactcttgca gccaggcagc agccgcagca taatccacag acgcaggcgc cgcaccaact
     4381 gtcgcaggag gacttgcaag cgcatgccaa tgtgataatg cgaaacgccg taatgaagcg
     4441 aaaaattgag gagcagacct cgaagcttat caatggcggt gccaagcatc aggcacagca
     4501 gcaatatcta aaccgtggcc agcaacgcca ggctcgacca gatgccaact cgaatgcctt
     4561 gctccatgcc ttgatctctg gcggtggcaa taaccacgcc tctggttatc ctatgaaagg
     4621 acagccgcag cagcatcatt ccaatttgag attcggtgat aaccaaaact tccaatcttt
     4681 tgagtccaac cagccgcact tcgccacgca gtacaagcag caataccaac agtctcagca
     4741 gcaacatccc catcagcagc cccaacagct gaattcgctt catcagaaca acgctggagc
     4801 tgtcaatagc ttcaataagg cccaaatgca agctcaatca gctatttcca tgctgcccaa
     4861 cagcggggat gagtttcatt aagcggccgc tcgagtctag agggcccgcg gttcgaaggt
     4921 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
     4981 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc
     5041 tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc
     5101 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5161 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     5221 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     5281 ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     5341 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     5401 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     5461 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     5521 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     5581 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     5641 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     5701 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     5761 tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6001 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6241 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6421 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     6901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     7201 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7441 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7501 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7561 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     7621 gaggcccttt cgt
//
