LOCUS       sequence-289355-        7825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCup-del419-770-4Alal-mut.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Igreja C, Izaurralde E
  TITLE     CUP promotes deadenylation and inhibits decapping of mRNA targets.
  JOURNAL   Genes Dev. 2011 Sep 15;25(18):1955-67. doi: 10.1101/gad.17136311.
  PUBMED    21937713
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2011-09-15- 15"; volume: "25"; 
            issue: "18"; pages: "1955-67"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmCup-del419-770-4Ala-mut_M
FEATURES             Location/Qualifiers
     source          1..7825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5110..5151
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5161..5178
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5201..5218)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5251..5385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5275..5294)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5329..5348
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6007..6595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6087..6106)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6766..7557,7558..7626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7389..7408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7627..7731)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7799..7817
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggaccct atgaaagagg aggcgatcga
     2761 attgaatggc catgctatgc aaatggccga agctgagcaa gaaaatggcg ctggcgccct
     2821 gaaaattgcc accaatgcgg gtgcaaccga tcgtccagcg catcagcagc ttccgctgcc
     2881 agtcgaagat cagcaggatg aggtgctgac ccccgctgag aagggcaagt tcgagtaccc
     2941 gccccctcca ccgccaccaa ctccagtcca ggctccgcta gccaccaagg ccaccgctct
     3001 gaatgccagt caagagcatg acgacgacga ggccaactcc gagaagtggg aggatccgtg
     3061 cgctccgccc ccgcctcctc cgctgccgac tagcgctttc ctggccaccg gactgggcta
     3121 cctcaagctg cccgccttca agctgaagga cgcactggag aaggccatca ccaagttgga
     3181 ggccaacaag cggacgctaa aggccagccc tgagtcttct cgttccatca aaaacaagaa
     3241 tgtggtggcc ctggagatgt tacccaggcg ctcaaaccca gaaacgatcg gcgatggatc
     3301 catgttggca tccacatcca cggctgttat gctgcagacc aaaaagcccg ccgttattgt
     3361 ggagatggag aggcgttgta agatcatcaa tttactggcc aaacagaatc aaatcctcga
     3421 atccatttcg ggggaggcca ttccgatgca tggaccctcg aagcatctgc acgaggacga
     3481 gggtctgacc ctgcaagttc tctcggcccg agcatccaca ccgtacacac agccctccag
     3541 catgttgtcc tgcacggccg ttagttgtga cctggagcac gattcgccgc ggaagcaagt
     3601 ggccagcaag gaggccgtgc cggagcagca gtctagtcag gtgcagcaaa agcgtccacc
     3661 cagcacaggc attcacaagc caggaagtct gcgagcaccc aaggctgttc gtcctactac
     3721 tgctccggtg gtcagcagca agccggtcaa gagcgctacc cgctcccgcg cgatggacat
     3781 tcgcaacggg atgttcaatg ccctgatgca caggtccaag gagagctttg tgatgcctcg
     3841 catcgccacc tgcgacgaca ttgaggcgga gggaagggct cgccgcatga atatttggcg
     3901 cacctcggac ggcacaagat tccggacccg ctccaccaca gctaacttaa acatgaacaa
     3961 caacaacaac aatgagtgca tgcctggaca gatgccttcg gtggatcagc tggaggccaa
     4021 atggcgtcga aactcactga acaatgttgg cgagactgcc aacaagcaga ctgataactt
     4081 ccagaagcta attgggtctc tttcttcggc taagccacag tcacaggcgg ttggctatga
     4141 tgccatctcc aactttataa tgcagcaaca gcagtaccag cagcagcaac agaagcagca
     4201 cctcatcata cagcagcagc agcaacagac cgcctttttg gcgagcctgc agctgaaggc
     4261 gatcctcggc cgggccgaca cccaattgct actgctccgt ctgactaaag gggaaatatc
     4321 caagcacgga ttgctggtgc agttggccaa cccacgcttg acggacatgg atcgcgaggc
     4381 catcactgct gtattgcagt tcaccaacac ccagcagcag cagcagcagc acaagcagca
     4441 gctggacatg ctctcaagca cggtcatcgc cagccagttg cagaatctcc acaacttggc
     4501 cattgtccag cagactcttg cagccaggca gcagccgcag cataatccac agacgcaggc
     4561 gccgcaccaa ctgtcgcagg aggacttgca agcgcatgcc aatgtgataa tgcgaaacgc
     4621 cgtaatgaag cgaaaaattg aggagcagac ctcgaagctt atcaatggcg gtgccaagca
     4681 tcaggcacag cagcaatatc taaaccgtgg ccagcaacgc caggctcgac cagatgccaa
     4741 ctcgaatgcc ttgctccatg ccttgatctc tggcggtggc aataaccacg cctctggtta
     4801 tcctatgaaa ggacagccgc agcagcatca ttccaatttg agattcggtg ataaccaaaa
     4861 cttccaatct tttgagtcca accagccgca cttcgccacg cagtacaagc agcaatacca
     4921 acagtctcag cagcaacatc cccatcagca gccccaacag ctgaattcgc ttcatcagaa
     4981 caacgctgga gctgtcaata gcttcaataa ggcccaaatg caagctcaat cagctatttc
     5041 catgctgccc aacagcgggg atgagtttca ttaagcggcc gctcgagtct agagggcccg
     5101 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     5161 catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctaag
     5221 gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata cattgatgag
     5281 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     5341 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     5401 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     5461 ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga gagccttcaa
     5521 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     5581 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     5641 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     5701 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     5761 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     5821 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     5881 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     5941 atcgctcgcg gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6001 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6061 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6181 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6301 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6361 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6421 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6481 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6541 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6601 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6661 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6721 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6781 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6841 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6901 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6961 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7021 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7081 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7141 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7201 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7261 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7321 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7381 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7441 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7501 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7561 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7621 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7681 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7741 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     7801 taggcgtatc acgaggccct ttcgt
//
