LOCUS       sequence-28939-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Chou MY, Rooke N, Turck CW, Black DL
  TITLE     hnRNP H is a component of a splicing enhancer complex that activates
            a c-src alternative exon in neuronal cells.
  JOURNAL   Mol Cell Biol. 1999 Jan . 19(1):69-77.
  PUBMED    9858532
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Jan . 19(1):69-77."
COMMENT     Sequence Label: pET15b-hnRNP-F
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aaattccctc tagaatattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg ctcgagatga
      121 tgctgggccc tgagggaggt gaaggctttg tggtcaagct ccgtggcctg ccctggtcct
      181 gctctgttga ggacgtgcag aacttcctct ctgactgcac gattcatgat ggggccgcag
      241 gtgtccattt catctacact agagagggca ggcagagtgg tgaggctttt gttgaacttg
      301 gatcagaaga tgatgtaaaa atggccctga aaaaagacag ggaaagcatg ggacaccggt
      361 acattgaggt gttcaagtcc cacagaaccg agatggattg ggtgttgaag cacagtggtc
      421 ccaacagtgc cgacagcgcc aacgatggct tcgtgcggct tcgaggactc ccatttggat
      481 gcacaaagga agaaattgtt cagttcttct cagggttgga aattgtgcca aacgggatca
      541 cattgcctgt ggaccccgaa ggcaagatta caggggaagc gttcgtgcag tttgcctcgc
      601 aggagttagc tgagaaggct ctagggaaac acaaggagag gatagggcac aggtacattg
      661 aggtgtttaa gagcagccag gaggaagtta ggtcatactc agatccccct ctgaagttca
      721 tgtccgtgca
//
