LOCUS       sequence-289396-        8263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO1-del835-886-GSG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Boland A, Huntzinger E, Schmidt S, Izaurralde E, Weichenrieder O
  TITLE     Crystal structure of the MID-PIWI lobe of a eukaryotic Argonaute 
            protein.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10466-71. doi: 
            10.1073/pnas.1103946108. Epub 2011 Jun 6.
  PUBMED    21646546
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1103946108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-06-28- 28"; volume: "108"; issue: "26"; pages: 
            "10466-71"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAGO1-del835-886-GSG_M
FEATURES             Location/Qualifiers
     source          1..8263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2685..2711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5548..5589
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5599..5616
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5639..5656)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5689..5823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5713..5732)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5767..5786
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6445..7033)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6525..6544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7204..7995,7996..8064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7827..7846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8065..8169)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8237..8255
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgaattc tatgtaccca tacgatgttc
     2701 cagattacgc tatgtccacg gagcgtgagc tggctcccgg tgggccagct cagctccatc
     2761 cgcacacgct gccgctgact tttccggatc tccagatgac ctccaccgtg ggcatcattg
     2821 ggaaagtcta cgagtcacag tggaccccct cgcccactcg gcctcagagt ccctctcagg
     2881 cgcagactag cttcgacacg ctcacatcac caccagcgcc cggctcgtcg gtcaatccca
     2941 ccgccgtcac cagcccaagt gcccagaatg tggccgctgg tggagcaact gtggccggtg
     3001 cagcagcaac tgccgcccag gtggcctccg ccttgggtgc caccaccggc agcgtgacgc
     3061 cagcaattgc caccgcaacg ccagccacgc agccggatat gcccgtcttt acgtgtccac
     3121 gacgcccgaa tctcggacga gagggtcgcc cgattgtgct gcgcgccaat cacttccagg
     3181 tgacaatgcc gcgtggctat gtgcatcact atgacatcaa tatacagccg gacaagtgtc
     3241 cgcgaaaggt gaaccgtgag attatcgaga ctatggtgca tgcctatagc aagatcttcg
     3301 gagtgctcaa gccggtgttc gatggtcgca acaatctgta tacccgcgat cccctgccca
     3361 ttggcaacga gcgtctagag ctggaggtta ctctacccgg cgagggcaaa gatcgaatct
     3421 ttcgcgtgac gatcaagtgg caggctcagg tctcgctctt caatttggag gaagctctcg
     3481 aaggccgcac gcggcagata ccctatgatg ccattttggc gctcgatgtg gtcatgcgcc
     3541 atctgcccag catgacgtac acgccagtgg gacgtagctt cttcagttcc ccggagggtt
     3601 actaccatcc cctgggtggt ggacgcgagg tttggttcgg tttccatcag agcgcaaggc
     3661 cctcgcagtg gaagatgatg ctcaatatcg atgtctcggc caccgctttc tacaaggctc
     3721 aaccagtcat tgacttcatg tgcgaggtgc tggacattcg cgacatcaac gagcagcgca
     3781 aaccgctcac cgattcgcag cgcgtcaagt tcacgaagga gatcaagggt ttgaagatcg
     3841 agatcaccca ctgcggccag atgcgtcgca agtatcgtgt gtgcaacgtc actcgccgcc
     3901 ccgctcagat gcaatcattc ccactgcagc tggagaacgg acagaccgta gagtgcaccg
     3961 tggccaagta cttcctggac aagtaccgca tgaaattgcg ctacccgcac ttgccctgcc
     4021 tgcaggttgg ccaagagcac aagcacactt acctgcctct agaggtgtgc aacattgtgg
     4081 ccggacagcg gtgcattaaa aagctgaccg atatgcagac gtcgaccatg atcaaggcca
     4141 cagctcgttc agctccggat cgtgagcgtg agattaacaa cttggtaaag cgcgccgact
     4201 tcaacaacga ttcgtatgtg caagagtttg gcctgaccat ctccaattcg atgatggagg
     4261 tacgaggacg cgtcttgcct cctcccaagc ttcagtatgg gggacgtgtg tctaccggcc
     4321 tcaccggcca gcagctgttc ccgccacaga acaaggtgag cttggcctcg cccaaccagg
     4381 gtgtatggga tatgcgaggc aagcagttct tcactggcgt cgagatccgc atctgggcca
     4441 tcgcctgttt tgccccacag cgcacggtgc gcgaggatgc gctgcgtaat ttcacccagc
     4501 agctgcagaa gatctcaaac gatgcaggca tgccgataat tggacagccg tgcttctgta
     4561 agtacgccac cgggccggat caagtggaac ccatgttccg ttacctgaag atcaccttcc
     4621 ccggcctgca actcgtcgtg gttgtgctgc ccggcaagac tccagtatac gccgaggtga
     4681 agcgtgtagg tgacaccgtt ctgggtatgg ccacccagtg tgtgcaggcc aagaacgtga
     4741 acaagacatc gccacagacg ctctctaatc tgtgtctgaa gatcaacgtc aagttgggcg
     4801 gcatcaattc aattctggtc ccctccattc ggccaaaggt cttcaatgag ccggtcatct
     4861 ttttgggtgc cgatgtgaca cacccaccag ctggcgacaa caagaaacca tcgattgccg
     4921 ccgtcgtggg ctccatggat gcccatccat cgcggtatgc cgccaccgtt cgcgtacagc
     4981 agcaccgaca ggagatcatc caggagctga gcagcatggt gcgcgagctg ttgatcatgt
     5041 tctacaagtc gacgggcggc tacaagcccc accgcatcat actctatcgt gacggagtct
     5101 ccgagggaca attcccacat gtcctgcaac acgaattgac cgccattcgc gaggcctgca
     5161 ttaagctaga gccagagtat cggccgggca tcacattcat tgtggtgcag aagggtagcg
     5221 gtccctcgca ctaccacgtt ctgtgggacg acaatcactt tgactcggac gagctgcagt
     5281 gcctcacgta tcagctatgc cgtacgtacg tgcgctgcac ccgatccgtc agtataccgg
     5341 cgccagccta ctacgcccat ttagtggcct tccgtgccag atatcatctg gtggagaagg
     5401 agcacgattc gggcgagggt tcgcaccaga gcggctgctc agaggatcgt acgccaggtg
     5461 ccatggccag ggccatcact gtgcacgcgg ataccaagaa ggtcatgtac tttgcctaac
     5521 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
     5581 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     5641 tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga tccagacatg
     5701 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     5761 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     5821 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     5881 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca gtcgaccgat
     5941 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     6001 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     6061 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     6121 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     6181 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     6241 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     6301 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     6361 tcagggacag cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg
     6421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6481 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6541 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6601 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     6661 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6721 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6781 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6841 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     6901 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6961 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7141 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7201 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7261 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7321 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7381 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7441 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7501 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7561 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7621 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7681 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7741 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7801 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     7861 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7921 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7981 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8041 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8101 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8161 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     8221 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgt
//
