LOCUS       sequence-289429-        8390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmLarp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8390)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmLarp_N
FEATURES             Location/Qualifiers
     source          1..8390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5675..5716
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5726..5743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5766..5783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5816..5950
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5840..5859)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5894..5913
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6572..7160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6652..6671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7331..8122,8123..8191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7954..7973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8192..8296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8364..8382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatgg gcacccacta ctttggcaac gtgcccgccg cctacatcga
     2761 attggacgct aacagcatca aggaggccat caagaagcaa gttgaatact atttcagcgt
     2821 cgataatctt accggcgact tctttttgcg ccgcaaaatg gaccccgagg gctatatccc
     2881 agttactctg atcgcctcgt tccatcgcgt tctcgcactg accaccgatg tggctgtgat
     2941 cgtaaacgcc attaaggaat cggataaact ggagctcttt gagggctata aggtgcgcac
     3001 caagacgaca ccgaccacct ggcccatcac ggaagtgccg gaggtcaccg aaggggaacc
     3061 gaaggctatt ggcacactgg agcaggagca gttggagcag aatgatggtc aggaaaagct
     3121 ggaggagcag actgaggcgg attccccgcc gcccattctc acatcggcga tggccacaaa
     3181 gccgctgaac agcattccgc cgccgccaat gccgcgaaac ccccagaatc tggtgctcaa
     3241 aatgttgcag gataagcaac agtcgagatc ctcgaccatt gcggctctaa actctgtgaa
     3301 tgcgatcagt gcgctgaccc agcaagtgga gggtggtgcc gctgagctgg ctggacattt
     3361 gagtggactg gctgagagtg tgaagcccaa gtcgacatcg acgccagaca agagaaatgc
     3421 tgcttcagcg ggcaatggag ctgggtcagc agctgctttg gtggcggagc cggagggcat
     3481 atggaaagag gtaaagagac gctccaaaac aaatgctatc aaagaaaacg ctactacacc
     3541 accacaacaa caacagccac cgctttcaca aacgctcaac aacaataacg ataatgtcaa
     3601 aacgaacaac acctcgtcca agtccaagtc ctcgtccaac aacgccccat ccaacgcctc
     3661 atcatcagcc actgtgtgtg taaccacgaa caatgcttcc tcagccacca aagcaaccac
     3721 caaaaccaca accacctcca ccgccactac caccacgaac aacaacatta aatctggcaa
     3781 tgctgcttac tccaaaaccc actccaaatc ctcatccaaa acagctgctc cgccatccgc
     3841 tcagtgccat gccgaaaagg aagaactaga cttccagttt gacgaggagc taatggaccc
     3901 cctaccccct ggaaccggac gcatcaacaa cttcaccgaa aacttctccg acgacgacga
     3961 atccgactac gagtttgccg atcgtgacat taacaagcta ctgattgtgg cccaagtggg
     4021 ccgcgcacct aagcacgagg gctacgaccg caccgccgac tttacgtcgc gtacgaaaat
     4081 cacccaggat ctggagaaca tcatcaacga tggactggtt aactacgaag aggatctgtg
     4141 gaccaccacc aatgtggttg ccgattacaa gactgtcaat gtgatttccc aggcagactt
     4201 cgaaaaactg gctggtggtc gaaacaaatc ggtgttgccg ccgcaggtgg tgccaccacc
     4261 gccaccattt gaggaagatc tggatgaaac cctggtgggt gacaccactc tcaactccac
     4321 gctgaacaac actctcaagt cccgacgtgc ccgcttctat gctgctccca actcgcactc
     4381 gattgatccc cgaacgccaa gaaagaggaa actccgtcac accgccaatc ctccagtgga
     4441 ggcccacgtc ggttggctgc ttgacactgt ggagcatcgt cctcgcacca cctcgatggg
     4501 ttcctcggcg ggcacatcgc ctaccgcctc gtcgtacgga tcgttcggat cgtcggtgcc
     4561 acagtcgctg cccgtattcc agcatccatc acatgctctt ctcaaggaga acaacttcac
     4621 ccaacaggcc taccacaagt accattcgcg ctgcctcaag gagcgccggc gcttgggcta
     4681 cggtcagtcg caggagatga acaccctgta ccgcttctgg tcgttcttcc tgcgcgagaa
     4741 cttcaacaag agcatgtaca acgagttccg atcgttggcg ctggaggatg ccggcaatgg
     4801 tttccgctac ggtcttgaat gcctcttccg gttcttctcg tacggcttgg agaagaagtt
     4861 cagaccgaat atctatcagg atttccagga cgagacgatt gccgattatg aaacaggtca
     4921 actgtacggc ctcgagaagt tctgggcctt cctgaaatac tacaagaatg gtgagaagtt
     4981 ggaggtgcag cccaagctgg ccgagtacct gaagagcttc aagaacattg aggacttccg
     5041 cgtggtggag ccggagatca acgagatgct gcaaggagtg ggcagcctga atccaggccg
     5101 ccagctcaat cgccaccgca gtgtgtcgga gagcgatggc acagctgtaa ttgcggccgg
     5161 cggtcgtcgc ctcaacacga ccatcaccaa ccgcagcgac tacgtgggca ggttgctgca
     5221 gcaacagcac caacaacagc agcagcagca gcatcaccag tatcagcagg gatacggcgg
     5281 ctacaatcag cagcaaaacc gcaggcgcac aggctccttc ggcagcacca cagtgcgcat
     5341 tcgcagcggc tccctgggca acaagccgca ggtggccaat cgcaatcagg gatcgcagca
     5401 cgagttgcga cgcggcggca gcaattctgg cctggcgcca cacaagcgac agcagcaaca
     5461 gaagccgaag ccaggagcag gatcacagac aggctcaaca cgtgccacca catcagcggc
     5521 agctacagca acaacagccg catcagcagc aacatcaaca gcagcgacgc cagcggtaac
     5581 ggtatcttcg gggtcatcgt cgtcgaagaa gtagaattct gcagatatcc agcacagtgg
     5641 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     5701 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     5761 atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc
     5821 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     5881 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     5941 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     6001 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtc
     6061 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     6121 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     6181 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     6241 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     6301 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     6361 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     6421 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     6481 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccag caaaaggcca
     6541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     6781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7621 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     7681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7921 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     7981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8341 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
