LOCUS       sequence-289434-        7892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF1_92-896-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7892)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF1_92-896-siRNAres-V5Hi...
FEATURES             Location/Qualifiers
     source          1..7892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3937..3946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5177..5218
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5228..5245
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5268..5285)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5318..5452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5342..5361)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5396..5415
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6074..6662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6154..6173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6833..7624,7625..7693))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7456..7475
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7694..7798)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7866..7884
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat cgtgaaggag ctgccgccgc atgcgtgcaa
     2761 gtattgcggc atccatgatc cagccacggt ggtcatgtgc aacaactgcc gcaaatggtt
     2821 ttgcaacggt cgtggaagca cttccggttc gcacatcatc aaccatctgg tgagggccaa
     2881 gcatcgcgag gtgacgctcc acggggaagg tcccctgggc gagacaatcc tggagtgcta
     2941 ctcctgtggt gtgcgcaacg tctttgtgct gggcttcatt ccggccaagg ccgattctgt
     3001 ggtcgtgctg ctctgccgtc agccgtgtgc cgcccaaaac tcccttaaag acatgaactg
     3061 ggaccaggaa cagtggaagc ctctaattgc agaccgctgc tttttggcct ggctggtaaa
     3121 gcaacccagc gaacagggac agctgcgagc tcgccaaatc tcagccgctc agatcaacaa
     3181 gctggaggag ctatggaagg agaatattga ggccacgttt caggatctgg agaagccagg
     3241 cattgactcg gagccagcac atgtgctact ccgctacgag gatggctatc agtacgagaa
     3301 gacctttggg ccgctggtcc gccttgaggc cgaatacgac caaaaactga aggagtctgc
     3361 cacgcaggag aacatcgaag tacgctggga cgtcggcctc aacaaaaaga ccattgccta
     3421 ctttacgctg gcgaagaccg attcggacat gaagctcatg catggcgacg agctgcgcct
     3481 gcattatgtg ggcgagctgt acaatccgtg gagcgagatc ggccacgtta tcaaggtgcc
     3541 ggacaatttc ggcgatgacg tcggcctgga gctgaaatcc tcaacgaatg ccccggttaa
     3601 gtgcaccagt aactttacgg tggacttcat ctggaagtgc acgtcatttg atcgcatgac
     3661 acgtgctctg tgcaaattcg ccatcgatcg caattcagtg tcgaacttca tctactcgcg
     3721 cctgttgggc cacggtcgtg cggattccaa cgacgaggtg ctgttccgcg gcccacaacc
     3781 caagctcttc agtgccccgc atctgccgga tttgaatcgc agccaggtgt atgccgtgaa
     3841 acacgcgctt cagcgtccgc tctcgctaat ccaagggccg cctggcacgg gcaaaaccgt
     3901 gacctcggcg accatcgttt accagctggt caagctccat ggtggcacag tgctggtgtg
     3961 cgctcccagc aacacggccg tggatcagct aactgagaag atccaccgaa caaaccttaa
     4021 agtggtgcgt gtttgcgcca agagccgtga ggccatcgat agcccggtaa gcttcctggc
     4081 gctgcacaac caaatccgta acatggagac caactcggag ctaaagaagc tgcagcagct
     4141 gaaagacgag accggcgagc tgagctcagc agacgaaaag cgataccgca acctgaaacg
     4201 tgccgccgag aaccaactgc tggaggctgc cgacgttatc tgctgcacat gcgtaggcgc
     4261 cggcgatggt cgtctatcgc gagtcaagtt cacctcgatc ctgatcgatg agtctatgca
     4321 gtcgacggag ccggagtgca tggtgccagt ggtgctgggc gctaagcagc tgatcctcgt
     4381 gggcgatcac tgccagctgg gaccggttgt tatgtgcaag aaagcagctc gtgccggcct
     4441 ctcgcaaagt ttgttcgagc gcctggtggt tctgggcatc cgtccgttcc ggctggaggt
     4501 gcaatatcgc atgcaccccg agctgtccca gttcccgtcc aacttcttct acgagggatc
     4561 gctgcaaaac ggcgtctgcg cggaggatcg tcgccttaag cttgatttcc cctggccaca
     4621 gccggagaga ccgatgttct ttctggtaac acagggacag gaggagattg ccggctccgg
     4681 cacctctttc ctcaaccgca cagaggcggc caacgtggaa aagattacga cgcgattcct
     4741 taaggcaggc atcaagccgg aacaaattgg aatcatcacg ccttacgaag gtcagcgcgc
     4801 ttacctggtg cagtacatgc aataccaggg cagcctgcac tctcgtctat accaggagat
     4861 cgagatcgcc agtgtggacg cgttccaggg acgtgagaag gacatcatta tcatgtcttg
     4921 cgtgcgatct aacgaacgtc aaggcatcgg ctttttgaac gacccacgtc gccttaatgt
     4981 tgcccttaca cgggccaagt tcgggatcat cattgtgggc aatcccaaag tgctcgccaa
     5041 gcagcagctg tggaaccatc tgctcaactt ctacaaggac cgtaaggtgc tcgtcgaggg
     5101 atctctgaat aaccttaagg agtcgctaat ccacttccag aagccccgct cgagtctaga
     5161 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     5221 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
     5281 ttctaaggcc tgagctcgct gatcagcctc gatcgaggat ccagacatga taagatacat
     5341 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     5401 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     5461 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     5521 gtaaaacctc tacaaatgtg gtatggctga ttatgatcag tcgaccgatg cccttgagag
     5581 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     5641 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     5701 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     5761 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     5821 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     5881 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     5941 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     6001 ttcaaggatc gctcgcggct cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg
     6061 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6121 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6181 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6241 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     6301 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6361 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6421 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6481 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6541 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6601 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6661 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6721 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6781 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6841 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6901 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6961 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7021 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7081 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7141 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7201 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7261 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7321 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7381 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7441 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7501 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7561 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7621 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7681 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7741 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7801 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     7861 ataaaaatag gcgtatcacg aggccctttc gt
//
