LOCUS       sequence-289435-        8522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF1_92-1106-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8522)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF1_92-1106-siRNAres-V5H...
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3937..3946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5807..5848
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5858..5875
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5898..5915)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5948..6082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5972..5991)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6026..6045
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6704..7292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6784..6803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7463..8254,8255..8323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8086..8105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8324..8428)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8496..8514
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat cgtgaaggag ctgccgccgc atgcgtgcaa
     2761 gtattgcggc atccatgatc cagccacggt ggtcatgtgc aacaactgcc gcaaatggtt
     2821 ttgcaacggt cgtggaagca cttccggttc gcacatcatc aaccatctgg tgagggccaa
     2881 gcatcgcgag gtgacgctcc acggggaagg tcccctgggc gagacaatcc tggagtgcta
     2941 ctcctgtggt gtgcgcaacg tctttgtgct gggcttcatt ccggccaagg ccgattctgt
     3001 ggtcgtgctg ctctgccgtc agccgtgtgc cgcccaaaac tcccttaaag acatgaactg
     3061 ggaccaggaa cagtggaagc ctctaattgc agaccgctgc tttttggcct ggctggtaaa
     3121 gcaacccagc gaacagggac agctgcgagc tcgccaaatc tcagccgctc agatcaacaa
     3181 gctggaggag ctatggaagg agaatattga ggccacgttt caggatctgg agaagccagg
     3241 cattgactcg gagccagcac atgtgctact ccgctacgag gatggctatc agtacgagaa
     3301 gacctttggg ccgctggtcc gccttgaggc cgaatacgac caaaaactga aggagtctgc
     3361 cacgcaggag aacatcgaag tacgctggga cgtcggcctc aacaaaaaga ccattgccta
     3421 ctttacgctg gcgaagaccg attcggacat gaagctcatg catggcgacg agctgcgcct
     3481 gcattatgtg ggcgagctgt acaatccgtg gagcgagatc ggccacgtta tcaaggtgcc
     3541 ggacaatttc ggcgatgacg tcggcctgga gctgaaatcc tcaacgaatg ccccggttaa
     3601 gtgcaccagt aactttacgg tggacttcat ctggaagtgc acgtcatttg atcgcatgac
     3661 acgtgctctg tgcaaattcg ccatcgatcg caattcagtg tcgaacttca tctactcgcg
     3721 cctgttgggc cacggtcgtg cggattccaa cgacgaggtg ctgttccgcg gcccacaacc
     3781 caagctcttc agtgccccgc atctgccgga tttgaatcgc agccaggtgt atgccgtgaa
     3841 acacgcgctt cagcgtccgc tctcgctaat ccaagggccg cctggcacgg gcaaaaccgt
     3901 gacctcggcg accatcgttt accagctggt caagctccat ggtggcacag tgctggtgtg
     3961 cgctcccagc aacacggccg tggatcagct aactgagaag atccaccgaa caaaccttaa
     4021 agtggtgcgt gtttgcgcca agagccgtga ggccatcgat agcccggtaa gcttcctggc
     4081 gctgcacaac caaatccgta acatggagac caactcggag ctaaagaagc tgcagcagct
     4141 gaaagacgag accggcgagc tgagctcagc agacgaaaag cgataccgca acctgaaacg
     4201 tgccgccgag aaccaactgc tggaggctgc cgacgttatc tgctgcacat gcgtaggcgc
     4261 cggcgatggt cgtctatcgc gagtcaagtt cacctcgatc ctgatcgatg agtctatgca
     4321 gtcgacggag ccggagtgca tggtgccagt ggtgctgggc gctaagcagc tgatcctcgt
     4381 gggcgatcac tgccagctgg gaccggttgt tatgtgcaag aaagcagctc gtgccggcct
     4441 ctcgcaaagt ttgttcgagc gcctggtggt tctgggcatc cgtccgttcc ggctggaggt
     4501 gcaatatcgc atgcaccccg agctgtccca gttcccgtcc aacttcttct acgagggatc
     4561 gctgcaaaac ggcgtctgcg cggaggatcg tcgccttaag cttgatttcc cctggccaca
     4621 gccggagaga ccgatgttct ttctggtaac acagggacag gaggagattg ccggctccgg
     4681 cacctctttc ctcaaccgca cagaggcggc caacgtggaa aagattacga cgcgattcct
     4741 taaggcaggc atcaagccgg aacaaattgg aatcatcacg ccttacgaag gtcagcgcgc
     4801 ttacctggtg cagtacatgc aataccaggg cagcctgcac tctcgtctat accaggagat
     4861 cgagatcgcc agtgtggacg cgttccaggg acgtgagaag gacatcatta tcatgtcttg
     4921 cgtgcgatct aacgaacgtc aaggcatcgg ctttttgaac gacccacgtc gccttaatgt
     4981 tgcccttaca cgggccaagt tcgggatcat cattgtgggc aatcccaaag tgctcgccaa
     5041 gcagcagctg tggaaccatc tgctcaactt ctacaaggac cgtaaggtgc tcgtcgaggg
     5101 atctctgaat aaccttaagg agtcgctaat ccacttccag aagcccaaaa agcttgtcaa
     5161 cagcatgaac attggggcac actttatgtc caccattatt gccgatgcca aggaagtgat
     5221 ggtgccaggc tccatttacg accgcagtgg cggttacggc caaggtcgcc aaatggtggg
     5281 acagtcaatg aatggcggac agtacggtgg cagtggaggt ggtccctacg gaaactcacc
     5341 cctcggctac ggtactccca gctccaattc catggtgggc tttggcctgg gcaacggagg
     5401 caatggcgcg gccggtggca acaacaactt cggaggggct gaacccagtt gggcggctgc
     5461 ccacctccac cacgactcca ttggctatat atccaacgag catggagcag cagcactggg
     5521 caacatgcca gttccggttg gcatgttcat gaacatgagc aatattccgc cgcgtttcta
     5581 caaccagcac cagcaggcga tcatggcggt caagcagaat cgcgccattc aacaacagac
     5641 gggtaatttc tctcccggta actcgggtcc tggagtcact ggagtcggag tcggacgaag
     5701 cgccacccca ggcggcaata agaagaccaa caagctggga aaatcgcgcg taacgggcgg
     5761 tggaactggc ggggcacgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     5821 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
     5881 ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct gatcagcctc
     5941 gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     6001 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     6061 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     6121 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     6181 ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     6241 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     6301 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     6361 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     6421 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     6481 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     6541 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     6601 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     6661 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6721 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6781 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6841 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     6901 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6961 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7021 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7081 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7141 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7201 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7261 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7321 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7381 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7441 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7501 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7561 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7621 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7681 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7741 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     7801 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7861 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7921 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7981 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8041 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     8101 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8161 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8221 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8281 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8341 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8401 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8461 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8521 gt
//
