LOCUS       sequence-289436-        8630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF1_92-1142-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8630)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF1_92-1142-siRNAres-V5H...
FEATURES             Location/Qualifiers
     source          1..8630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      3937..3946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5915..5956
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5966..5983
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6006..6023)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6056..6190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6080..6099)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6134..6153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6812..7400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6892..6911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7571..8362,8363..8431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8194..8213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8432..8536)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8604..8622
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat cgtgaaggag ctgccgccgc atgcgtgcaa
     2761 gtattgcggc atccatgatc cagccacggt ggtcatgtgc aacaactgcc gcaaatggtt
     2821 ttgcaacggt cgtggaagca cttccggttc gcacatcatc aaccatctgg tgagggccaa
     2881 gcatcgcgag gtgacgctcc acggggaagg tcccctgggc gagacaatcc tggagtgcta
     2941 ctcctgtggt gtgcgcaacg tctttgtgct gggcttcatt ccggccaagg ccgattctgt
     3001 ggtcgtgctg ctctgccgtc agccgtgtgc cgcccaaaac tcccttaaag acatgaactg
     3061 ggaccaggaa cagtggaagc ctctaattgc agaccgctgc tttttggcct ggctggtaaa
     3121 gcaacccagc gaacagggac agctgcgagc tcgccaaatc tcagccgctc agatcaacaa
     3181 gctggaggag ctatggaagg agaatattga ggccacgttt caggatctgg agaagccagg
     3241 cattgactcg gagccagcac atgtgctact ccgctacgag gatggctatc agtacgagaa
     3301 gacctttggg ccgctggtcc gccttgaggc cgaatacgac caaaaactga aggagtctgc
     3361 cacgcaggag aacatcgaag tacgctggga cgtcggcctc aacaaaaaga ccattgccta
     3421 ctttacgctg gcgaagaccg attcggacat gaagctcatg catggcgacg agctgcgcct
     3481 gcattatgtg ggcgagctgt acaatccgtg gagcgagatc ggccacgtta tcaaggtgcc
     3541 ggacaatttc ggcgatgacg tcggcctgga gctgaaatcc tcaacgaatg ccccggttaa
     3601 gtgcaccagt aactttacgg tggacttcat ctggaagtgc acgtcatttg atcgcatgac
     3661 acgtgctctg tgcaaattcg ccatcgatcg caattcagtg tcgaacttca tctactcgcg
     3721 cctgttgggc cacggtcgtg cggattccaa cgacgaggtg ctgttccgcg gcccacaacc
     3781 caagctcttc agtgccccgc atctgccgga tttgaatcgc agccaggtgt atgccgtgaa
     3841 acacgcgctt cagcgtccgc tctcgctaat ccaagggccg cctggcacgg gcaaaaccgt
     3901 gacctcggcg accatcgttt accagctggt caagctccat ggtggcacag tgctggtgtg
     3961 cgctcccagc aacacggccg tggatcagct aactgagaag atccaccgaa caaaccttaa
     4021 agtggtgcgt gtttgcgcca agagccgtga ggccatcgat agcccggtaa gcttcctggc
     4081 gctgcacaac caaatccgta acatggagac caactcggag ctaaagaagc tgcagcagct
     4141 gaaagacgag accggcgagc tgagctcagc agacgaaaag cgataccgca acctgaaacg
     4201 tgccgccgag aaccaactgc tggaggctgc cgacgttatc tgctgcacat gcgtaggcgc
     4261 cggcgatggt cgtctatcgc gagtcaagtt cacctcgatc ctgatcgatg agtctatgca
     4321 gtcgacggag ccggagtgca tggtgccagt ggtgctgggc gctaagcagc tgatcctcgt
     4381 gggcgatcac tgccagctgg gaccggttgt tatgtgcaag aaagcagctc gtgccggcct
     4441 ctcgcaaagt ttgttcgagc gcctggtggt tctgggcatc cgtccgttcc ggctggaggt
     4501 gcaatatcgc atgcaccccg agctgtccca gttcccgtcc aacttcttct acgagggatc
     4561 gctgcaaaac ggcgtctgcg cggaggatcg tcgccttaag cttgatttcc cctggccaca
     4621 gccggagaga ccgatgttct ttctggtaac acagggacag gaggagattg ccggctccgg
     4681 cacctctttc ctcaaccgca cagaggcggc caacgtggaa aagattacga cgcgattcct
     4741 taaggcaggc atcaagccgg aacaaattgg aatcatcacg ccttacgaag gtcagcgcgc
     4801 ttacctggtg cagtacatgc aataccaggg cagcctgcac tctcgtctat accaggagat
     4861 cgagatcgcc agtgtggacg cgttccaggg acgtgagaag gacatcatta tcatgtcttg
     4921 cgtgcgatct aacgaacgtc aaggcatcgg ctttttgaac gacccacgtc gccttaatgt
     4981 tgcccttaca cgggccaagt tcgggatcat cattgtgggc aatcccaaag tgctcgccaa
     5041 gcagcagctg tggaaccatc tgctcaactt ctacaaggac cgtaaggtgc tcgtcgaggg
     5101 atctctgaat aaccttaagg agtcgctaat ccacttccag aagcccaaaa agcttgtcaa
     5161 cagcatgaac attggggcac actttatgtc caccattatt gccgatgcca aggaagtgat
     5221 ggtgccaggc tccatttacg accgcagtgg cggttacggc caaggtcgcc aaatggtggg
     5281 acagtcaatg aatggcggac agtacggtgg cagtggaggt ggtccctacg gaaactcacc
     5341 cctcggctac ggtactccca gctccaattc catggtgggc tttggcctgg gcaacggagg
     5401 caatggcgcg gccggtggca acaacaactt cggaggggct gaacccagtt gggcggctgc
     5461 ccacctccac cacgactcca ttggctatat atccaacgag catggagcag cagcactggg
     5521 caacatgcca gttccggttg gcatgttcat gaacatgagc aatattccgc cgcgtttcta
     5581 caaccagcac cagcaggcga tcatggcggt caagcagaat cgcgccattc aacaacagac
     5641 gggtaatttc tctcccggta actcgggtcc tggagtcact ggagtcggag tcggacgaag
     5701 cgccacccca ggcggcaata agaagaccaa caagctggga aaatcgcgcg taacgggcgg
     5761 tggaactggc ggggcaccgc taacacaagg aagctcggta tgcaatgctg ctccatacag
     5821 tcagcacccg atgcctttgt cgctgcagat gacccagccc agcggatttg ctctgtccca
     5881 gcagcgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     5941 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     6001 atcagcctcg actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc
     6061 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6121 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6181 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     6241 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtc
     6301 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     6361 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     6421 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     6481 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     6541 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
     6601 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
     6661 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
     6721 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccag caaaaggcca
     6781 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6841 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6901 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6961 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     7021 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7081 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7141 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7201 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     7261 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7321 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7381 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7441 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7501 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7561 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7621 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7681 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7741 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7801 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7861 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
     7921 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7981 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8041 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8101 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8161 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     8221 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8281 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8341 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8401 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8461 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8521 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     8581 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
