LOCUS       sequence-28944-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Vopalensky P, Ruzickova J, Pavlu B, Kozmik Z
  TITLE     A lens-specific co-injection marker for medaka transgenesis.
  JOURNAL   Biotechniques. 2010 Mar . 48(3):235-6.
  PUBMED    20359307
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2010 Mar . 48(3):235-6."
COMMENT     Sequence Label: p817-mgammaFcry-EGFP
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(429..449)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(429..447)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(491..507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(491..507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(504..526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    515..531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(539..545,546..563,564..569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    584..605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(722..739)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcgg caagctcctt gaattcgaat cgatgggaag
       61 cttggctggt gttggcaggt cggatgggat ggtgctgttg agtgtgtggg gctgggagca
      121 gggtctatat agcaggaggt ctgctgtgtt ggcaggaaca gcacaaaagg ggcccgcagt
      181 gggacagggc actttttttc ccctctcttt tctatataat gactatgggg gaggtgtggg
      241 ttattgtatg ttttatctta gactctccag agctttcgaa tggatgacaa atcactgacc
      301 tggttaaaaa ggtcacttga tgtagcttgg gccgtcgagc ccatcctcta gagtcgacct
      361 gcaggcattg caagcttcgg actagttcta gagcggccgc caccgcggtg gagctccagc
      421 ttttgttccc tttagtgagg gttaattgcg cgcattaccc tgttatccct acgcgcttgg
      481 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      541 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      601 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      661 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      721 cctcgctcac tgactcgctg cgctcggtcg ttc
//
