LOCUS       sequence-289446-        8354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF2_1-960-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8354)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF2_1-960-siRNAres-V5His6_N
FEATURES             Location/Qualifiers
     source          1..8354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5639..5680
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5690..5707
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5730..5747)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5780..5914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5804..5823)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5858..5877
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6536..7124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6616..6635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7295..8086,8087..8155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7918..7937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8156..8260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8328..8346
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgctagc caacgattct gcaacgactg acagcgaggt
     2761 gtccacaccg ccgtcgtcga ggaaagattt ggcagatggt gttgccgagg gtgacaacga
     2821 caacgacatc gataccggcg acacggacgc aaatgcggat gaagatgtgg atgcggctgc
     2881 tgccattgcc ctggaggccg aggagcgcga ggagctgcaa cagttcataa gcgagctgcg
     2941 ggaaaaaatt gaaagtaagc gacagctgcg cctccaaaac tccacaatcg agctgcccgg
     3001 tgaggagtac ttcgccaaac tggactccaa tctcaaaaag aacacggcat tcgtcaagaa
     3061 actgaaacta ttcacggcca cacagctgga cggactattg cgtgagatat ccgctctaaa
     3121 cctgagcaaa tacatctcgg aaatatgcgc cgcgctggtc gaggccaaac tgaaaatgac
     3181 tgatgtgccg gcggtggtga cgctggcctc tcggctgcac tgcacatatg ccgacttcga
     3241 tgtccatttt ctggaggcct ggcaaaaggc actgaacatc aaggccaccg agaagattgg
     3301 caatccgagc aaactgcgcg ttgatcttcg cctgtttgcg gaactagtca gctctggtgt
     3361 gatccaaatg aagcccggat tggcccaact gggtgttgtt ctggtgcatt tgattgccct
     3421 ggacaaggat gaccacagca acttctcgat catcctctca ttctgccggc actgcggcga
     3481 ggagtacgcc ggcctagtgc cgcaaaagat gcaacagttg gccaccaaat acggggtgga
     3541 ggtgccgaaa tccgacttcc tgactgccga caagcagctc aatctgcgca ccatgcttaa
     3601 gggttacttc aaggccctgt gcaagcatgt cctggccgag caggccgaac tgatgaacat
     3661 gaccaagaac attcggcgca ccatggagtg caagggtgag atatccacgg agaagcgcga
     3721 gaagtgtgaa ctgatgcagg ccggattcga taagctgctg gcctccgctc aatcgctgtc
     3781 ggaactgctg ggtgaaccac tgcccgagct ggccaaggag tctgagggct gtaatccggg
     3841 cacagtcatc gacaacatgc tggacagtgc ctcctttggt gtgctggatc cgtggggcga
     3901 cgaggagacc cgagccttct acacggattt gcccgatttg agacagtttc tgccaaactt
     3961 ttcggcaccc aaagtcgatt tggaaacgtt agaggagccc agtgaactaa ccgaagaagc
     4021 gatcgatgcc aatctagatg ccgaaatgga tctagatgat ccaccgtcca ccacttcgga
     4081 tacgactccg gagaatccaa tcgaagagca gccaacgacg ccagttgccg ctgcggagga
     4141 tctcaagccg cagaagatgg gtaacgctct gatggaactg ggtcgccagc agcagcagca
     4201 aagtcagctc aaccagaatc cgatccagat ccaaaaccag atgcgtcagc agttcgatgg
     4261 cttcctggtc aacctattca actgtgtgaa caaggagctg atcgattcgg cggccatcga
     4321 gttcctgctt aacttcaata ccaagcatca acgcaagaag ctgacgcgta cgatattctc
     4381 ggtgcagcgt acgcgtctgg atatcctgcc ctatctctcc cgcttcgtgg ccattgtgca
     4441 tatgtgcaat accgatgtgg ccaccgattt ggccgaactg ctgcgcaagg agttcaagtg
     4501 gcacattcac aagaagaacc agctgaatat cgagacgaag cttaagattg tgcgattcat
     4561 tggtgaactg gttaagtttg gtttgtttaa aaagttcgat gccttaggat gtctgaagat
     4621 gctgctaagg gactttcagc atcatcaaat cgagatggcc tgtgcctttg tggaagtcac
     4681 cggcgtctat ctgtacaatt gccgggatgc ccgtctgctg atgaatgtgt tcctggacca
     4741 aatgctgcgc ttgaaaacag ccacggcaat ggactcgcga catgcggcgc agattgagag
     4801 cgtgtactat ttggtaaagc cgccggaatc gtccaagcga gagcccatgg ttcggccggc
     4861 aatgcatgag tacatccgtt acttgatctt cgaggagctt tgcaaacaga acgttgatcg
     4921 ttgtatcaaa atgctgcgcc gcatcgattg gcaggatccg gagacaaatt gttatgccat
     4981 taagtgcctc agcaaggcgt atctgctgcg cttccagctc attcgctgcc tggccgatct
     5041 cgtatccgga ctgagctcct atcagccacg agcggttacc atcgtgatcg ataatgtgtt
     5101 tgaggatata cgcgctggcc tcgagattca ttcgccacgc atggcacagc ggcgcatcgc
     5161 gatggccaag tatctgggcg agatgtacaa ctacaagctg gtggagtcca cgaacattct
     5221 taatacgctg tactccatca tctcgctggg cgtatcgaat gaccaaaatg tcgtctcgcc
     5281 actggatccg cccgatagcc tgttccgact gaagcttgcc tgtatgctac tggacacgtg
     5341 tgcaccctac ttcaccagcc aggccacgcg aaaaaaactg gactactttc tggtcttctt
     5401 ccaacactac tattggttca agaaatccca tcccgtgttc agcaaaacgg agaacacctc
     5461 cgatttgttc cccatcctaa tggatcacac ctatcgcgac tgtctactca atgtgcggcc
     5521 caagctgaag atttacaaga gtctagagca ggccaaggcg gcgattgatc atctgcagga
     5581 gaagctctat ccgcagctaa agggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     5641 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     5701 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg
     5761 ctgatcagcc tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac
     5821 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     5881 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     5941 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     6001 tggtatggct gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct
     6061 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     6121 tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     6181 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     6241 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     6301 ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     6361 tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     6421 ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     6481 ctcttaccag ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6721 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6961 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     7621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     7921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8281 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8341 cgaggccctt tcgt
//
