LOCUS       sequence-289447-        8186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF2_57-960-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8186)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF2_57-960-siRNAres-V5Hi...
FEATURES             Location/Qualifiers
     source          1..8186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5471..5512
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5522..5539
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5562..5579)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5612..5746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5636..5655)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5690..5709
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6368..6956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6448..6467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7127..7918,7919..7987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7750..7769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7988..8092)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8160..8178
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctctggaggc cgaagagcgc gaggagctgc aacagttcat
     2761 aagcgagctg cgggaaaaaa ttgaaagtaa gcgacagctg cgcctccaaa actccacaat
     2821 cgagctgccc ggtgaggagt acttcgccaa actggactcc aatctcaaaa agaacacggc
     2881 attcgtcaag aaactgaaac tattcacggc cacacagctg gacggactat tgcgtgagat
     2941 atccgctcta aacctgagca aatacatctc ggaaatatgc gccgcgctgg tcgaggccaa
     3001 actgaaaatg actgatgtgc cggcggtggt gacgctggcc tctcggctgc actgcacata
     3061 tgccgacttc gatgtccatt ttctggaggc ctggcaaaag gcactgaaca tcaaggccac
     3121 cgagaagatt ggcaatccga gcaaactgcg cgttgatctt cgcctgtttg cggaactagt
     3181 cagctctggt gtgatccaaa tgaagcccgg attggcccaa ctgggtgttg ttctggtgca
     3241 tttgattgcc ctggacaagg atgaccacag caacttctcg atcatcctct cattctgccg
     3301 gcactgcggc gaggagtacg ccggcctagt gccgcaaaag atgcaacagt tggccaccaa
     3361 atacggggtg gaggtgccga aatccgactt cctgactgcc gacaagcagc tcaatctgcg
     3421 caccatgctt aagggttact tcaaggccct gtgcaagcat gtcctggccg agcaggccga
     3481 actgatgaac atgaccaaga acattcggcg caccatggag tgcaagggtg agatatccac
     3541 ggagaagcgc gagaagtgtg aactgatgca ggccggattc gataagctgc tggcctccgc
     3601 tcaatcgctg tcggaactgc tgggtgaacc actgcccgag ctggccaagg agtctgaggg
     3661 ctgtaatccg ggcacagtca tcgacaacat gctggacagt gcctcctttg gtgtgctgga
     3721 tccgtggggc gacgaggaga cccgagcctt ctacacggat ttgcccgatt tgagacagtt
     3781 tctgccaaac ttttcggcac ccaaagtcga tttggaaacg ttagaggagc ccagtgaact
     3841 aaccgaagaa gcgatcgatg ccaatctaga tgccgaaatg gatctagatg atccaccgtc
     3901 caccacttcg gatacgactc cggagaatcc aatcgaagag cagccaacga cgccagttgc
     3961 cgctgcggag gatctcaagc cgcagaagat gggtaacgct ctgatggaac tgggtcgcca
     4021 gcagcagcag caaagtcagc tcaaccagaa tccgatccag atccaaaacc agatgcgtca
     4081 gcagttcgat ggcttcctgg tcaacctatt caactgtgtg aacaaggagc tgatcgattc
     4141 ggcggccatc gagttcctgc ttaacttcaa taccaagcat caacgcaaga agctgacgcg
     4201 tacgatattc tcggtgcagc gtacgcgtct ggatatcctg ccctatctct cccgcttcgt
     4261 ggccattgtg catatgtgca ataccgatgt ggccaccgat ttggccgaac tgctgcgcaa
     4321 ggagttcaag tggcacattc acaagaagaa ccagctgaat atcgagacga agcttaagat
     4381 tgtgcgattc attggtgaac tggttaagtt tggtttgttt aaaaagttcg atgccttagg
     4441 atgtctgaag atgctgctaa gggactttca gcatcatcaa atcgagatgg cctgtgcctt
     4501 tgtggaagtc accggcgtct atctgtacaa ttgccgggat gcccgtctgc tgatgaatgt
     4561 gttcctggac caaatgctgc gcttgaaaac agccacggca atggactcgc gacatgcggc
     4621 gcagattgag agcgtgtact atttggtaaa gccgccggaa tcgtccaagc gagagcccat
     4681 ggttcggccg gcaatgcatg agtacatccg ttacttgatc ttcgaggagc tttgcaaaca
     4741 gaacgttgat cgttgtatca aaatgctgcg ccgcatcgat tggcaggatc cggagacaaa
     4801 ttgttatgcc attaagtgcc tcagcaaggc gtatctgctg cgcttccagc tcattcgctg
     4861 cctggccgat ctcgtatccg gactgagctc ctatcagcca cgagcggtta ccatcgtgat
     4921 cgataatgtg tttgaggata tacgcgctgg cctcgagatt cattcgccac gcatggcaca
     4981 gcggcgcatc gcgatggcca agtatctggg cgagatgtac aactacaagc tggtggagtc
     5041 cacgaacatt cttaatacgc tgtactccat catctcgctg ggcgtatcga atgaccaaaa
     5101 tgtcgtctcg ccactggatc cgcccgatag cctgttccga ctgaagcttg cctgtatgct
     5161 actggacacg tgtgcaccct acttcaccag ccaggccacg cgaaaaaaac tggactactt
     5221 tctggtcttc ttccaacact actattggtt caagaaatcc catcccgtgt tcagcaaaac
     5281 ggagaacacc tccgatttgt tccccatcct aatggatcac acctatcgcg actgtctact
     5341 caatgtgcgg cccaagctga agatttacaa gagtctagag caggccaagg cggcgattga
     5401 tcatctgcag gagaagctct atccgcagct aaagggcggc cgctcgagtc tagagggccc
     5461 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     5521 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa
     5581 ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga
     5641 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     5701 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     5761 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     5821 cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca
     5881 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     5941 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     6001 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     6061 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     6121 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     6181 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     6241 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     6301 gatcgctcgc ggctcttacc agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6541 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     6601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6781 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     6841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7141 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7201 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7261 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7321 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7381 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7441 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7501 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7561 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7621 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7681 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7741 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7801 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7861 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7921 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7981 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8041 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8101 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8161 ataggcgtat cacgaggccc tttcgt
//
