LOCUS       sequence-289448-        9029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF2_57-1241-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9029)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF2_57-1241-siRNAres-V5H...
FEATURES             Location/Qualifiers
     source          1..9029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6314..6355
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6365..6382
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6405..6422)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6455..6589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6479..6498)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6533..6552
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7211..7799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7291..7310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7970..8761,8762..8830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8593..8612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8831..8935)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9003..9021
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctctggaggc cgaagagcgc gaggagctgc aacagttcat
     2761 aagcgagctg cgggaaaaaa ttgaaagtaa gcgacagctg cgcctccaaa actccacaat
     2821 cgagctgccc ggtgaggagt acttcgccaa actggactcc aatctcaaaa agaacacggc
     2881 attcgtcaag aaactgaaac tattcacggc cacacagctg gacggactat tgcgtgagat
     2941 atccgctcta aacctgagca aatacatctc ggaaatatgc gccgcgctgg tcgaggccaa
     3001 actgaaaatg actgatgtgc cggcggtggt gacgctggcc tctcggctgc actgcacata
     3061 tgccgacttc gatgtccatt ttctggaggc ctggcaaaag gcactgaaca tcaaggccac
     3121 cgagaagatt ggcaatccga gcaaactgcg cgttgatctt cgcctgtttg cggaactagt
     3181 cagctctggt gtgatccaaa tgaagcccgg attggcccaa ctgggtgttg ttctggtgca
     3241 tttgattgcc ctggacaagg atgaccacag caacttctcg atcatcctct cattctgccg
     3301 gcactgcggc gaggagtacg ccggcctagt gccgcaaaag atgcaacagt tggccaccaa
     3361 atacggggtg gaggtgccga aatccgactt cctgactgcc gacaagcagc tcaatctgcg
     3421 caccatgctt aagggttact tcaaggccct gtgcaagcat gtcctggccg agcaggccga
     3481 actgatgaac atgaccaaga acattcggcg caccatggag tgcaagggtg agatatccac
     3541 ggagaagcgc gagaagtgtg aactgatgca ggccggattc gataagctgc tggcctccgc
     3601 tcaatcgctg tcggaactgc tgggtgaacc actgcccgag ctggccaagg agtctgaggg
     3661 ctgtaatccg ggcacagtca tcgacaacat gctggacagt gcctcctttg gtgtgctgga
     3721 tccgtggggc gacgaggaga cccgagcctt ctacacggat ttgcccgatt tgagacagtt
     3781 tctgccaaac ttttcggcac ccaaagtcga tttggaaacg ttagaggagc ccagtgaact
     3841 aaccgaagaa gcgatcgatg ccaatctaga tgccgaaatg gatctagatg atccaccgtc
     3901 caccacttcg gatacgactc cggagaatcc aatcgaagag cagccaacga cgccagttgc
     3961 cgctgcggag gatctcaagc cgcagaagat gggtaacgct ctgatggaac tgggtcgcca
     4021 gcagcagcag caaagtcagc tcaaccagaa tccgatccag atccaaaacc agatgcgtca
     4081 gcagttcgat ggcttcctgg tcaacctatt caactgtgtg aacaaggagc tgatcgattc
     4141 ggcggccatc gagttcctgc ttaacttcaa taccaagcat caacgcaaga agctgacgcg
     4201 tacgatattc tcggtgcagc gtacgcgtct ggatatcctg ccctatctct cccgcttcgt
     4261 ggccattgtg catatgtgca ataccgatgt ggccaccgat ttggccgaac tgctgcgcaa
     4321 ggagttcaag tggcacattc acaagaagaa ccagctgaat atcgagacga agcttaagat
     4381 tgtgcgattc attggtgaac tggttaagtt tggtttgttt aaaaagttcg atgccttagg
     4441 atgtctgaag atgctgctaa gggactttca gcatcatcaa atcgagatgg cctgtgcctt
     4501 tgtggaagtc accggcgtct atctgtacaa ttgccgggat gcccgtctgc tgatgaatgt
     4561 gttcctggac caaatgctgc gcttgaaaac agccacggca atggactcgc gacatgcggc
     4621 gcagattgag agcgtgtact atttggtaaa gccgccggaa tcgtccaagc gagagcccat
     4681 ggttcggccg gcaatgcatg agtacatccg ttacttgatc ttcgaggagc tttgcaaaca
     4741 gaacgttgat cgttgtatca aaatgctgcg ccgcatcgat tggcaggatc cggagacaaa
     4801 ttgttatgcc attaagtgcc tcagcaaggc gtatctgctg cgcttccagc tcattcgctg
     4861 cctggccgat ctcgtatccg gactgagctc ctatcagcca cgagcggtta ccatcgtgat
     4921 cgataatgtg tttgaggata tacgcgctgg cctcgagatt cattcgccac gcatggcaca
     4981 gcggcgcatc gcgatggcca agtatctggg cgagatgtac aactacaagc tggtggagtc
     5041 cacgaacatt cttaatacgc tgtactccat catctcgctg ggcgtatcga atgaccaaaa
     5101 tgtcgtctcg ccactggatc cgcccgatag cctgttccga ctgaagcttg cctgtatgct
     5161 actggacacg tgtgcaccct acttcaccag ccaggccacg cgaaaaaaac tggactactt
     5221 tctggtcttc ttccaacact actattggtt caagaaatcc catcccgtgt tcagcaaaac
     5281 ggagaacacc tccgatttgt tccccatcct aatggatcac acctatcgcg actgtctact
     5341 caatgtgcgg cccaagctga agatttacaa gagtctagag caggccaagg cggcgattga
     5401 tcatctgcag gagaagctct atccgcagct aaagactacc aataatgctc aggatccgtc
     5461 gctgggcaca atcagtgaaa taagcgagat agacgagggc ggcactgatg aggattctgg
     5521 gtcatcgaat gatcagcgag agcgccaagt gtcgggccaa gagcaggacc agagcaacga
     5581 ctggacagag aacgaggcgg agccaccgct gccgccgccc ccgccgccag aaaagtccaa
     5641 ggaggacctg gagttcgagc aattgtacga aaaaatgacc acagactcgt accaagaacg
     5701 gcttaaggag ccgataaagg ctacggccaa ggatatacct gtgccgatga tggcgcgcct
     5761 gcaaaaaaaa tcgtacgatc agataacagg tgctcagacc atcggccctg ggatcaacaa
     5821 tatgtcacag atctcaaagt cgcttcccaa tcaggacgga acgcctggct ctccaggtgc
     5881 agattcacca gatggcaacg gcaaatccgg cggcggtgta ggtggtagcg atgttggtgc
     5941 tgctggtgct gctggtgctt ctggtgctac atctgtaccc tttgtgctaa tggtgcgcgg
     6001 ccacaagggc ggaaagcagc aatttaagtc atttgtggca ccatccgact cgcacttggc
     6061 caccaaccta aagcggcagg agcagatgat acgcgaggag aaggagaagg tcaagcgcct
     6121 tacgcttaac ataactgagc gcattgagga ggaggactac caggagtcgt tgatgccgcc
     6181 gcagcatcga agcttcaccc agagctacta ccagaagccc aataagcaca agttcaagca
     6241 ccaaaagggt gcgcccgatg cggacctcat atttcacggc ggccgctcga gtctagaggg
     6301 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     6361 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     6421 taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa gatacattga
     6481 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     6541 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     6601 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     6661 aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc ttgagagcct
     6721 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     6781 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     6841 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     6901 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     6961 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     7021 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     7081 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     7141 aaggatcgct cgcggctctt accagccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7201 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7261 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7321 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7381 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     7441 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7501 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7561 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7621 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7681 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7741 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7801 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7861 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7921 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7981 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8041 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8101 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8161 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8221 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8281 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8341 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8401 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8461 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8521 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8581 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8641 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8701 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8761 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8821 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8881 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8941 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     9001 aaaataggcg tatcacgagg ccctttcgt
//
