LOCUS       sequence-289449-        9197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF2-E801R-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9197)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF2-E801R-siRNAres-V5His6_N
FEATURES             Location/Qualifiers
     source          1..9197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6482..6523
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6533..6550
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6573..6590)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6623..6757
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6647..6666)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6701..6720
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7379..7967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7459..7478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8138..8929,8930..8998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8761..8780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8999..9103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9171..9189
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgctagc caacgattct gcaacgactg acagcgaggt
     2761 gtccacaccg ccgtcgtcga ggaaagattt ggcagatggt gttgccgagg gtgacaacga
     2821 caacgacatc gataccggcg acacggacgc aaatgcggat gaagatgtgg atgcggctgc
     2881 tgccattgcc ctggaggccg aggagcgcga ggagctgcaa cagttcataa gcgagctgcg
     2941 ggaaaaaatt gaaagtaagc gacagctgcg cctccaaaac tccacaatcg agctgcccgg
     3001 tgaggagtac ttcgccaaac tggactccaa tctcaaaaag aacacggcat tcgtcaagaa
     3061 actgaaacta ttcacggcca cacagctgga cggactattg cgtgagatat ccgctctaaa
     3121 cctgagcaaa tacatctcgg aaatatgcgc cgcgctggtc gaggccaaac tgaaaatgac
     3181 tgatgtgccg gcggtggtga cgctggcctc tcggctgcac tgcacatatg ccgacttcga
     3241 tgtccatttt ctggaggcct ggcaaaaggc actgaacatc aaggccaccg agaagattgg
     3301 caatccgagc aaactgcgcg ttgatcttcg cctgtttgcg gaactagtca gctctggtgt
     3361 gatccaaatg aagcccggat tggcccaact gggtgttgtt ctggtgcatt tgattgccct
     3421 ggacaaggat gaccacagca acttctcgat catcctctca ttctgccggc actgcggcga
     3481 ggagtacgcc ggcctagtgc cgcaaaagat gcaacagttg gccaccaaat acggggtgga
     3541 ggtgccgaaa tccgacttcc tgactgccga caagcagctc aatctgcgca ccatgcttaa
     3601 gggttacttc aaggccctgt gcaagcatgt cctggccgag caggccgaac tgatgaacat
     3661 gaccaagaac attcggcgca ccatggagtg caagggtgag atatccacgg agaagcgcga
     3721 gaagtgtgaa ctgatgcagg ccggattcga taagctgctg gcctccgctc aatcgctgtc
     3781 ggaactgctg ggtgaaccac tgcccgagct ggccaaggag tctgagggct gtaatccggg
     3841 cacagtcatc gacaacatgc tggacagtgc ctcctttggt gtgctggatc cgtggggcga
     3901 cgaggagacc cgagccttct acacggattt gcccgatttg agacagtttc tgccaaactt
     3961 ttcggcaccc aaagtcgatt tggaaacgtt agaggagccc agtgaactaa ccgaagaagc
     4021 gatcgatgcc aatctagatg ccgaaatgga tctagatgat ccaccgtcca ccacttcgga
     4081 tacgactccg gagaatccaa tcgaagagca gccaacgacg ccagttgccg ctgcggagga
     4141 tctcaagccg cagaagatgg gtaacgctct gatggaactg ggtcgccagc agcagcagca
     4201 aagtcagctc aaccagaatc cgatccagat ccaaaaccag atgcgtcagc agttcgatgg
     4261 cttcctggtc aacctattca actgtgtgaa caaggagctg atcgattcgg cggccatcga
     4321 gttcctgctt aacttcaata ccaagcatca acgcaagaag ctgacgcgta cgatattctc
     4381 ggtgcagcgt acgcgtctgg atatcctgcc ctatctctcc cgcttcgtgg ccattgtgca
     4441 tatgtgcaat accgatgtgg ccaccgattt ggccgaactg ctgcgcaagg agttcaagtg
     4501 gcacattcac aagaagaacc agctgaatat cgagacgaag cttaagattg tgcgattcat
     4561 tggtgaactg gttaagtttg gtttgtttaa aaagttcgat gccttaggat gtctgaagat
     4621 gctgctaagg gactttcagc atcatcaaat cgagatggcc tgtgcctttg tggaagtcac
     4681 cggcgtctat ctgtacaatt gccgggatgc ccgtctgctg atgaatgtgt tcctggacca
     4741 aatgctgcgc ttgaaaacag ccacggcaat ggactcgcga catgcggcgc agattgagag
     4801 cgtgtactat ttggtaaagc cgccggaatc gtccaagcga gagcccatgg ttcggccggc
     4861 aatgcatgag tacatccgtt acttgatctt cgaggagctt tgcaaacaga acgttgatcg
     4921 ttgtatcaaa atgctgcgcc gcatcgattg gcaggatccg gagacaaatt gttatgccat
     4981 taagtgcctc agcaaggcgt atctgctgcg cttccagctc attcgctgcc tggccgatct
     5041 cgtatccgga ctgagctcct atcagccacg agcggttacc atcgtgatcg ataatgtgtt
     5101 tgaggatata cgcgctggcc tccggattca ttcgccacgc atggcacagc ggcgcatcgc
     5161 gatggccaag tatctgggcg agatgtacaa ctacaagctg gtggagtcca cgaacattct
     5221 taatacgctg tactccatca tctcgctggg cgtatcgaat gaccaaaatg tcgtctcgcc
     5281 actggatccg cccgatagcc tgttccgact gaagcttgcc tgtatgctac tggacacgtg
     5341 tgcaccctac ttcaccagcc aggccacgcg aaaaaaactg gactactttc tggtcttctt
     5401 ccaacactac tattggttca agaaatccca tcccgtgttc agcaaaacgg agaacacctc
     5461 cgatttgttc cccatcctaa tggatcacac ctatcgcgac tgtctactca atgtgcggcc
     5521 caagctgaag atttacaaga gtctagagca ggccaaggcg gcgattgatc atctgcagga
     5581 gaagctctat ccgcagctaa agactaccaa taatgctcag gatccgtcgc tgggcacaat
     5641 cagtgaaata agcgagatag acgagggcgg cactgatgag gattctgggt catcgaatga
     5701 tcagcgagag cgccaagtgt cgggccaaga gcaggaccag agcaacgact ggacagagaa
     5761 cgaggcggag ccaccgctgc cgccgccccc gccgccagaa aagtccaagg aggacctgga
     5821 gttcgagcaa ttgtacgaaa aaatgaccac agactcgtac caagaacggc ttaaggagcc
     5881 gataaaggct acggccaagg atatacctgt gccgatgatg gcgcgcctgc aaaaaaaatc
     5941 gtacgatcag ataacaggtg ctcagaccat cggccctggg atcaacaata tgtcacagat
     6001 ctcaaagtcg cttcccaatc aggacggaac gcctggctct ccaggtgcag attcaccaga
     6061 tggcaacggc aaatccggcg gcggtgtagg tggtagcgat gttggtgctg ctggtgctgc
     6121 tggtgcttct ggtgctacat ctgtaccctt tgtgctaatg gtgcgcggcc acaagggcgg
     6181 aaagcagcaa tttaagtcat ttgtggcacc atccgactcg cacttggcca ccaacctaaa
     6241 gcggcaggag cagatgatac gcgaggagaa ggagaaggtc aagcgcctta cgcttaacat
     6301 aactgagcgc attgaggagg aggactacca ggagtcgttg atgccgccgc agcatcgaag
     6361 cttcacccag agctactacc agaagcccaa taagcacaag ttcaagcacc aaaagggtgc
     6421 gcccgatgcg gacctcatat ttcacggcgg ccgctcgagt ctagagggcc cgcggttcga
     6481 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
     6541 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc
     6601 tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca
     6661 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6721 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6781 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6841 atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca
     6901 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     6961 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     7021 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     7081 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     7141 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     7201 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     7261 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     7321 cggctcttac cagccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7381 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7561 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7801 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7861 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7921 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7981 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8041 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8101 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8161 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8221 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8281 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8341 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8401 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     8461 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8521 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8581 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8641 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8701 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     8761 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8821 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8881 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8941 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9001 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9061 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9121 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     9181 tcacgaggcc ctttcgt
//
